Tnfrii

A complete anthropometrical examination and a biochemical analysis were carried out on each patient. Height and weight were measured with the patient standing in light clothes and shoeless. BMI was calculated as body weight divided by height squared (kg/m²). Laboratory studies included glucose, insulin, glycated haemoglobin, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, and transaminases, all of which were analysed using a conventional automated analyser. Insulin resistance (IR) was estimated using homeostasis model assessment of IR (HOMA2-IR) [23 (link)]. Serum levels of adiponectin (Linco Research, Inc., St. Charles, USA), resistin (Biovendor, Modrice, Czech Republic), interleukin 6 (IL6) (Quantikine, R&D Systems, Minneapolis, USA), tumour necrosis factor receptor 2 (TNFRII) (AssayPro, St. Charles, USA), and C-reactive protein (CRP) (Dade Behring, Marburg, Germany) were measured in duplicate using ELISA, following the manufacturer's instructions.

Circulating levels of IL-17A (Enzo Life Sciences, Farmingdale, NY, USA) and several adipo/cytokines—IL-6 (Quantikine, R&D Systems, Minneapolis, MN, USA), adiponectin (EMD Millipore, St. Charles, MI, USA), resistin (BioVendor, Brno, Czech Republic), lipocalin-2 (R&D Systems, Minneapolis, MN, USA) and TNFRII (AssayPro, St. Charles, IL, USA)—were measured in duplicate using enzyme-linked immunosorbent assays (ELISA) following the manufacturer’s instructions. The IL-17A assay sensitivity was 0.201 pg/mL, and intra-assay and inter-assay coefficients of variation (CV) were 5.4% and 9.4%, respectively. IL-6 assay sensitivity was 0.039 pg/mL, and intra-assay and inter-assay CV were 7.4% and 7.8%, respectively. adiponectin assay sensitivity was 0.2 ng/mL, and intra-assay and inter-assay CV were 3.4% and 5.7%, respectively. resistin assay sensitivity was 0.012 ng/mL, and intra-assay and inter-assay CV were 5.9% and 7.6%, respectively. lipocalin-2 assay sensitivity was 0.012 ng/mL, and intra-assay and inter-assay CV were 3.7% and 6.5%, respectively. Finally, the TNFRII assay sensitivity was 0.1 ng/mL and the inter-assay and intra-assay coefficients of variation were less than 3.2% and 3.3%, respectively.