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Rabbit anti foxa2

Manufactured by Cell Signaling Technology
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Rabbit anti-FOXA2 is a primary antibody that recognizes the FOXA2 (forkhead box protein A2) transcription factor. FOXA2 is a key regulator of embryonic development and plays a role in the differentiation of various cell types, including hepatocytes, pancreatic beta cells, and neurons. This antibody can be used in applications such as Western blotting, immunohistochemistry, and immunocytochemistry to detect and study the expression of FOXA2 in biological samples.

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Lab products found in correlation

Goat anti sox2, by Santa Cruz Biotechnology (2 mentions) Goat anti oct3 4, by Santa Cruz Biotechnology (2 mentions) Mouse anti tra 1 81, by Merck Group (2 mentions) Mouse anti ssea4, by Cell Signaling Technology (2 mentions) Tcs sp5 laser scanning microscope, by Leica (2 mentions) Ecl western blotting detection reagent, by Cytiva (2 mentions) Goat anti foxa2, by R&D Systems (2 mentions) Rabbit anti foxa1, by Cell Signaling Technology (2 mentions) Mouse anti actb, by Cell Signaling Technology (2 mentions) Nitrocellulose pre cut blotting membranes, by Thermo Fisher Scientific (2 mentions) Goat anti pdx1, by R&D Systems (1 mentions) Mouse anti glucagon, by Merck Group (1 mentions) Mouse anti tra 1 60, by Cell Signaling Technology (1 mentions) Goat anti pdx1 antibody, by R&D Systems (1 mentions) Mouse anti ssea4, by Abcam (1 mentions) Goat anti sox17, by R&D Systems (1 mentions) Mouse anti nestin, by R&D Systems (1 mentions) Rabbit anti oct4, by Santa Cruz Biotechnology (1 mentions) Dylight secondary antibody, by Jackson ImmunoResearch (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

Close spelling variants of this product

Rabbit anti-FoxA2 antibody Anti-FOXA2 FOXA2

6 protocols using rabbit anti foxa2

1

Immunofluorescence Analysis of Stem Cell Markers

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Cells were fixed with 4% paraformaldehyde for 30 min and then permeabilized in PBS containing 0.2% Triton X-100. Cells were blocked with PBS containing 3% BSA, and incubated with primary antibodies overnight at 4 °C. Then the cells were incubated with the secondary antibodies for 1 h at room temperature after washing with PBS. Images were acquired on a TCS SP5 laser-scanning microscope (Leica). The following antibodies and dilutions were used: goat anti-OCT-3/4 (1:500, Cat #sc-8628, Santa Cruz), goat anti-SOX2 (1:500, Cat #sc-17320, Santa Cruz), mouse anti-TRA-1-81 (1:50, Cat #MAB4381, Millipore), mouse anti-SSEA4 (1:500, Cat #4755, Cell Signaling), rabbit anti-FOXA2 (1:250, Cat #8186, Cell Signaling), goat anti-SOX17 (1:500, Cat #GT15094, Acris/Novus), goat anti-PDX1 (1:500, Cat #AF2419, R&D Systems), rabbit anti-NKX6.1 (1:300, Cat #NBP1-82553, Acris/Novus), guinea pig anti-C-Peptide (1:100, Cat #ab30477, Abcam), mouse anti-Glucagon (1:500, Cat #G2654, Sigma), rat anti-Somatostatin (1:300, Cat #MA5-16987, Invitrogen).
Wang X., Sterr M., A.n.s.a.r.u.l.l.a.h., Burtscher I., Böttcher A., Beckenbauer J., Siehler J., Meitinger T., Häring H.U., Staiger H., Cernilogar F.M., Schotta G., Irmler M., Beckers J., Wright C.V., Bakhti M, & Lickert H. (2019). Point mutations in the PDX1 transactivation domain impair human β-cell development and function. Molecular Metabolism, 24, 80-97.
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2

Western Blot Analysis of FOXA Transcription Factors

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Cells were harvested and lysed with cell lysis buffer (Cell Signaling Technology, 9803) containing protease inhibitor (Roche, 05892791001) and 1 mM PMSF (MP Biomedicals, ICN19538105). Sample preparation follows NuPAGE Novex protocol. 10% Bis-Tris Gel (Novex, NP0301BOX) was used and transferred to Nitrocellulose Pre-Cut Blotting Membranes (Novex, LC2001). Blocking was performed with 5% milk in Tris-based saline with 0.1% Tween 20 (TBST) for 30 min at RT. After blocking, primary antibodies were diluted in blocking solution and incubated overnight at 4°C. The following antibodies were used with noted dilution ratios: goat anti-FOXA2 (R&D, AF2400, 1:1000), rabbit anti-FOXA2 (Cell Signaling Technology, 3143S, 1:1000), rabbit anti-FOXA1 (Cell Signaling Technology, 58613S, 1:1000), mouse anti-ACTB (Cell Signaling Technology, 3700S, 1:10,000). After washing with TBST, HRP conjugated secondary antibodies in blocking solution were incubated at RT for 1 hour and the gel was visualized using ECL western blotting detection reagent (Amersham, RPN2236). Antibodies for this study are summarized in Table S8.
Lee K., Cho H., Rickert R.W., Li Q.V., Pulecio J., Leslie C.S, & Huangfu D. (2019). FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation. Cell reports, 28(2), 382-393.e7.
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3

Immunofluorescence Characterization of Stem Cells

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Cells were fixed with 4% paraformaldehyde for 30 min and then permeabilized in PBS containing 0.2% Triton X-100. Cells were blocked with PBS containing 3% BSA, and incubated with primary antibodies overnight at 4 °C. Then secondary antibodies were incubated for 1 h at room temperature after washing with PBS. Images were acquired on a TCS SP5 laser-scanning microscope (Leica). The following antibodies and dilutions were used: goat anti-OCT-3/4 (1:500, #sc-8628, Santa Cruz), goat anti-SOX2 (1:500, #sc-17320, Santa Cruz), mouse anti-TRA-1-60 (1:1000, #4746, Cell Signaling), mouse anti-TRA-1-81 (1:50, MAB4381, Millipore), mouse anti-SSEA4 (1:500, #4755, Cell Signaling), rabbit anti-FOXA2 (1:250, #8186, Cell Signaling), goat anti-SOX17 antibody (1:500, #GT15094, Acris/Novus), goat anti-PDX1 antibody (1:500, #AF2419, R&D Systems).
Wang X., Sterr M., Burtscher I., Chen S., Hieronimus A., Machicao F., Staiger H., Häring H.U., Lederer G., Meitinger T., Cernilogar F.M., Schotta G., Irmler M., Beckers J., Hrabě de Angelis M., Ray M., Wright C.V., Bakhti M, & Lickert H. (2018). Genome-wide analysis of PDX1 target genes in human pancreatic progenitors. Molecular Metabolism, 9, 57-68.
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4

Immunocytochemistry for Pluripotency and Lineage Markers

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Cells were fixed with 4% paraformaldehyde (Sigma) in PBS, permeabilized/blocked in PBS with 0.1% Triton X-100 (Mallinckrodt Baker, Phillipsburg, NJ) and 1% bovine serum albumin (BSA; Sigma) for 30 min and incubated overnight at 4 °C with primary antibodies: Mouse anti-SSEA4 (AbCam, Cambridge, MA) and rabbit anti-OCT4 (SantaCruz Biotechnology Inc., Santa Cruz, CA), goat anti-SOX17 (R&D Systems, Minneapolis, MN), rabbit anti-FOXA2 (Cell Signaling Technology, Danvers, MA), rabbit anti-MOX1 (Novus Biologicals, Littleton, CO), mouse anti-KDR (PE-conjugated, R&D Systems), rabbit anti-β-Tubulin III (TUJ1, Sigma, St. Louis, MO), mouse anti-Nestin (R&D Systems). After three washes with PBS, cells were incubated with corresponding DyLight secondary antibodies (Jackson Immunoresearch Laboratories) for 1 h at room temperature. Nuclear DNA was stained with DAPI (Vectashield, Vector Laboratories, Burlingame, CA).
Fan Y., Zhang F, & Tzanakakis E.S. (2016). Engineering Xeno-Free Microcarriers with Recombinant Vitronectin, Albumin and UV Irradiation for Human Pluripotent Stem Cell Bioprocessing. ACS biomaterials science & engineering, 3(8), 1510-1518.
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5

Immunoblotting Analysis of Organoid Protein Expression

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Organoids were processed for immunoblotting as previously described [76 (link)]. Briefly, monolayers were incubated in lysis buffer (20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 10% glycerol, and 2x Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific)) on ice for 5 minutes and centrifuged at 14,000 rpm for 20 minutes. Samples were resolved on Bolt 4–12% Bis-Tris Plus Gels (Invitrogen), transferred onto polyvinylidene difluoride membranes, and blocked using 5% skim milk. The following antibodies were used for immunoblotting studies: mouse anti-β-actin (1:5,000, Sigma-Aldrich, AC-15), rabbit anti-ACE2 (1:1,000, Abcam, ab15348), mouse anti-TMPRSS2 (1:1,000, Santa Cruz Biotechnology, Dallas, TX, USA, sc-515727), rabbit anti-BRG1 (1:250, R&D Systems, MAB5738), mouse anti-FOXM1 (1:100, Santa Cruz Biotechnology, sc-271746), and rabbit anti-FOXA2 (1:200, Cell Signaling Technology, Danvers, MA, USA, 8186). Secondary antibodies (mouse anti-rabbit and goat-anti mouse, 211-032-171 and 115-035-174, respectively) were purchased from Jackson ImmunoResearch (West Grove, PA, USA).
Jang K.K., Kaczmarek M.E., Dallari S., Chen Y.H., Tada T., Axelrad J., Landau N.R., Stapleford K.A, & Cadwell K. (2022). Variable susceptibility of intestinal organoid–derived monolayers to SARS-CoV-2 infection. PLoS Biology, 20(3), e3001592.
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6

Western Blot Analysis of FOXA Transcription Factors

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Cells were harvested and lysed with cell lysis buffer (Cell Signaling Technology, 9803) containing protease inhibitor (Roche, 05892791001) and 1 mM PMSF (MP Biomedicals, ICN19538105). Sample preparation follows NuPAGE Novex protocol. 10% Bis-Tris Gel (Novex, NP0301BOX) was used and transferred to Nitrocellulose Pre-Cut Blotting Membranes (Novex, LC2001). Blocking was performed with 5% milk in Tris-based saline with 0.1% Tween 20 (TBST) for 30 min at RT. After blocking, primary antibodies were diluted in blocking solution and incubated overnight at 4°C. The following antibodies were used with noted dilution ratios: goat anti-FOXA2 (R&D, AF2400, 1:1000), rabbit anti-FOXA2 (Cell Signaling Technology, 3143S, 1:1000), rabbit anti-FOXA1 (Cell Signaling Technology, 58613S, 1:1000), mouse anti-ACTB (Cell Signaling Technology, 3700S, 1:10,000). After washing with TBST, HRP conjugated secondary antibodies in blocking solution were incubated at RT for 1 hour and the gel was visualized using ECL western blotting detection reagent (Amersham, RPN2236). Antibodies for this study are summarized in Table S8.
Lee K., Cho H., Rickert R.W., Li Q.V., Pulecio J., Leslie C.S, & Huangfu D. (2019). FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation. Cell reports, 28(2), 382-393.e7.
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