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Transwell cell culture 8 μm inserts

Manufactured by Merck Group
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Sourced in United States

The Transwell cell culture 8 μm inserts are a laboratory equipment product designed for cell culture applications. The inserts feature a semi-permeable membrane with 8 micron pores, allowing for the study of cell migration, invasion, and other cell-based assays.

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Lab products found in correlation

Tritc dextran, by Merck Group (2 mentions) Tritc dextran, by Agilent Technologies (2 mentions) Synergy 2 multi detection microplate reader, by Agilent Technologies (2 mentions) Axio observer z1 microscope, by Zeiss (1 mentions)

Close spelling variants of this product

Transwell cell culture inserts Cell culture inserts Transwell inserts Culture insert

3 protocols using transwell cell culture 8 μm inserts

1

Transwell Assay for Cell Migration

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Transwell cell culture 8 μm inserts (Millipore, USA) were coated with 0.1% gelatin. HUVECs transiently transfected with LOX-PP and EV for 48 h were seeded with 25,000 cells/well in the upper chamber of the transwell culture insert with 1% FBS containing EBM-2. In the lower chamber, 10 ng/mL of VEGF 165 A in 1% FBS containing EBM-2 was added separately in each well. The cells were incubated for 8 h. After incubation, the transwell culture inserts were washed and the cells were fixed in 4% paraformaldehyde and stained with Wright Giemsa, following staining the cells present in the upper side of the chamber was wiped with cotton swabs and cells attached to the lower chamber were imaged using Axio Observer Z.1 microscope (Carl Zeiss, Germany). The cells were counted in the five fields manually using NIH ImageJ analysis software39 (link).
Nareshkumar R.N., Sulochana K.N, & Coral K. (2018). Inhibition of angiogenesis in endothelial cells by Human Lysyl oxidase propeptide. Scientific Reports, 8, 10426.
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2

In Vitro BBB Permeability Assay

Cited 1 time
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An in vitro BBB model was established according to our recent report.41 (link) Briefly, Transwell cell culture 8 μm inserts (Millipore) were coated with 5 μg/cm2 fibronectin in 150 μL PBS (1.68 μL/mL stock solution in 150 μL PBS per insert). HBMECs (33,000 cells/well) were seeded with 1% fetal bovine serum (FBS) containing EBM-2 in the upper chamber of the Transwell culture insert. In the lower chamber, NHA cells were seeded with 45,000 cells/well. After TEER values reached ~100 Ω (usually 2–3 days after adding HBMECs), NPs were added into the top chamber. TRITC-dextran (10 μL, 1 mg/mL, 4.4 kDa; Sigma-Aldrich) was added to the top chamber after 1 h. As various time points, including 1, 2, 3, 4, 6, and 24 h after treatment, medium in the bottom chamber was sampled and subjected to measurement for the amount of TRITC-dextran using a BioTek Synergy 2 Multi-Detection Microplate Reader with an excitation/emission wavelength of 550/575 nm.
Liu F., Peng B., Li M., Ma J., Deng G., Zhang S., Sheu W.C., Zou P., Wu H., Liu J., Chen A.T., Mohammed F.S, & Zhou J. (2021). Targeted disruption of tumor vasculature via polyphenol nanoparticles to improve brain cancer treatment. Cell reports. Physical science, 3(1), 100691.
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3

In Vitro BBB Permeability Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
An in vitro BBB model was established according to our recent report.41 (link) Briefly, Transwell cell culture 8 μm inserts (Millipore) were coated with 5 μg/cm2 fibronectin in 150 μL PBS (1.68 μL/mL stock solution in 150 μL PBS per insert). HBMECs (33,000 cells/well) were seeded with 1% fetal bovine serum (FBS) containing EBM-2 in the upper chamber of the Transwell culture insert. In the lower chamber, NHA cells were seeded with 45,000 cells/well. After TEER values reached ~100 Ω (usually 2–3 days after adding HBMECs), NPs were added into the top chamber. TRITC-dextran (10 μL, 1 mg/mL, 4.4 kDa; Sigma-Aldrich) was added to the top chamber after 1 h. As various time points, including 1, 2, 3, 4, 6, and 24 h after treatment, medium in the bottom chamber was sampled and subjected to measurement for the amount of TRITC-dextran using a BioTek Synergy 2 Multi-Detection Microplate Reader with an excitation/emission wavelength of 550/575 nm.
Liu F., Peng B., Li M., Ma J., Deng G., Zhang S., Sheu W.C., Zou P., Wu H., Liu J., Chen A.T., Mohammed F.S, & Zhou J. (2021). Targeted disruption of tumor vasculature via polyphenol nanoparticles to improve brain cancer treatment. Cell reports. Physical science, 3(1), 100691.
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