Recombinant human mbl

Manufactured by Bio-Techne

Sourced in France

Recombinant human MBL is a protein produced using recombinant DNA technology. It is a member of the collectin family and functions as a pattern recognition receptor involved in the innate immune response.

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Lab products found in correlation

Human serum albumin (hsa), by Merck Group (1 mentions) C reactive protein (crp), by Merck Group (1 mentions) Purified human c1q, by Complement Technology (1 mentions)

2 protocols using recombinant human mbl

Purification and Labeling of Immune Proteins

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Human Clu was purified from plasma, as previously described.^{7 (link)} Recombinant human MBL and human and murine Clu were from Biotechne (Lille, France). HSA, C1q, and FITC-labeled WGA were from Sigma-Aldrich (St. Louis, MO, USA). SAP (Calbiochem, Darmstadt, Germany), CRP (Millipore, Billerica, MA, USA), and H1, H2A, H2B, H3, and H4 histone subunits (New England Biolabs, Ipswich, MA, USA) were from the indicated providers. Proteins were labeled with Oregon green 488 dye (FluoReporter Oregon Green 488 Protein Labeling Kit; Invitrogen Molecular Probes, Carlsbad, CA, USA) or biotinylated (EZ-Link Sulfo-NHS-LC-Biotin Kit; Pierce, Rockford, IL, USA) using the commercial kits. The origins and clone numbers of the mAbs used in this study are listed in Table 1.

Cunin P., Beauvillain C., Miot C., Augusto J.F., Preisser L., Blanchard S., Pignon P., Scotet M., Garo E., Fremaux I., Chevailler A., Subra J.F., Blanco P., Wilson M.R., Jeannin P, & Delneste Y. (2016). Clusterin facilitates apoptotic cell clearance and prevents apoptotic cell-induced autoimmune responses. Cell Death & Disease, 7(5), e2215-.

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Characterizing SARS-CoV-2 Immune Modulators

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Commercial and non-commercial recombinant SARS-CoV-2 proteins used in this study are listed in Supplementary Table 1. Recombinant human PTX3 and its domains from CHO cells were produced in house, as previously described (Bottazzi et al., 1997) . Recombinant human Surfactant Protein-A (SP-A) was from Origene. Recombinant human MBL, Collectin-12 (CLP-1), Ficolin-1, Ficolin-2, Ficolin-3 and Surfactant Protein-D (SP-D) were from Biotechne. Purified human C1q was from Complement Technology, purified CRP was from Millipore and purified SAP was purchased from Abcam. Rabbit anti-PTX3 antibody was produced in house (Bottazzi et al., 1997) , rabbit anti-MBL Ab was purchased from Abcam.
Anti-C1q polyclonal antibody was purchased from Dako. Anti-CRP and anti-SAP antibodies were from Merck.

Stravalaci M., Pagani I., Paraboschi E.M., Pedotti M., Doni A., Scavello F., Mapelli S.N., Sironi M., Varani L., Matkovic M., Cavalli A., Cesana D., Gallina P., Pedemonte N., Capurro V., Clementi N., Mancini N., Invernizzi P., Rappuoli R., Duga S., Bottazzi B., Uguccioni M., Asselta R., Vicenzi E., Mantovani A., & Garlanda C. (2021). Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules.

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