To identify exosome markers, primary antibodies against CD63 and TSG101 were purchased from Abcam (Cambridge, UK), and primary antibodies against Hsp 70 and Hsp 90 were obtained from Cell Signaling Technology (CST, Beverly, MA, USA). The secondary antibodies were F(ab)2 fragments of donkey anti-mouse immunoglobulin or donkey anti-rabbit immunoglobulin linked to horseradish peroxidase (Jackson ImmunoResearch, Inc., USA). Immunoblotting reagents from an electrochemiluminescence kit were used (Amersham Biosciences, Uppsala, Sweden).
Immunoblotting reagents from an electrochemiluminescence kit
Manufactured by Cytiva
Sourced in Sweden
Immunoblotting reagents from an electrochemiluminescence kit. The core function of these reagents is to enable the detection and analysis of target proteins through electrochemiluminescence-based Western blotting techniques.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase, by Jackson ImmunoResearch (3 mentions)
Nupage lds sample buffer, by Thermo Fisher Scientific (2 mentions)
F ab 2 fragments of donkey anti mouse immunoglobulin, by Jackson ImmunoResearch (2 mentions)
Donkey anti rabbit immunoglobulin, by Jackson ImmunoResearch (2 mentions)
Primary antibodies against hsp70, by Cell Signaling Technology (2 mentions)
Tsg101, by Abcam (2 mentions)
Primary antibodies against cd63 and tsg101, by Abcam (1 mentions)
Primary antibodies against hsp 70 and hsp 90, by Cell Signaling Technology (1 mentions)
F ab 2 fragments of donkey anti mouse immunoglobulin or donkey anti rabbit immunoglobulin, by Jackson ImmunoResearch (1 mentions)
3 protocols using immunoblotting reagents from an electrochemiluminescence kit
1
Exosome Protein Marker Detection
2
Exosome Marker Identification Protocol
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Total protein was extracted with 1× NuPAGE LDS sample buffer (Thermo Fisher Scientific) according to manufacturer’s instructions. To identify exosome markers, primary antibodies against TLR4, CD63, and TSG101 were purchased from Abcam (Cambridge, UK), and primary antibodies against HSP70 were obtained from Cell Signaling Technology (CST; Beverly, MA, USA). The secondary antibodies were F(ab)2 fragments of donkey anti-mouse immunoglobulin or donkey anti-rabbit immunoglobulin linked to horseradish peroxidase (Jackson ImmunoResearch, USA). Immunoblotting reagents from an electrochemiluminescence kit were used (Amersham Biosciences, Uppsala, Sweden).
3
Identifying Exosome Markers via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was extracted with 1 times NuPAGE LDS Sample Buffer (Thermo Fisher Scientific), according to the manufacturer’s instructions. To identify exosome markers, primary antibodies against TLR4, CD63, and TSG101 were purchased from Abcam (Cambridge, UK), and primary antibodies against HSP70 were obtained from Cell Signaling Technology (CST; Beverly, MA, USA). The secondary antibodies were f(ab)2 fragments of donkey anti-mouse immunoglobulin or donkey anti-rabbit immunoglobulin linked to horseradish peroxidase (Jackson ImmunoResearch, USA). Immunoblotting reagents from an electrochemiluminescence kit were used (Amersham Biosciences, Uppsala, Sweden).
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