Anti f4 80 efluor450

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-F4/80 efluor450 is a flow cytometry reagent used for the detection and analysis of F4/80 positive cells. It is a monoclonal antibody conjugated with the efluor450 fluorescent dye, which allows for the identification and quantification of F4/80 expressing cells in various sample types.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

F4/80 (353 citations) Anti-F4/80 (122 citations) F4/80-eFluor450 (14 citations) EFluor450-conjugated anti-F4/80 (clone: (2 citations) Anti-F4/80 eFluor450 mAb (clone BM8; (2 citations)

6 protocols using anti f4 80 efluor450

Comprehensive Immune Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were incubated on ice for 45 min in Fc block in the presence of relevant primary antibodies. The anti-CD11c FITC, anti-CD11b PE, anti-CD103 PECy5, anti-MHCII APC-efluor780, anti-F4/80 efluor450, anti-CD45 BV510, anti-CX3CR1 PECy7, anti-Sca1 PE, anti-CD16/32 PerCP, anti-CD34 efluor450, anti-B220 FITC, anti-Gr1 FITC, anti-Ter119 FITC, anti-CD3 FITC, and anti-CD127 APC were purchased from eBioscience. The anti-CD11b PE, anti-CD11c APC, anti-Ly6C FITC, anti-CD117 PECy7 and anti-CD43 PerCP were purchased from BD Bioscience. After staining, cells were analyzed by flow cytometry on a FACS Canto. Data were analyzed with the FlowJo software.

Ibáñez L., Pontier-Bres R., Larbret F., Rekima A., Verhasselt V., Blin-Wakkach C, & Czerucka D. (2019). Saccharomyces boulardii Strain CNCM I-745 Modifies the Mononuclear Phagocytes Response in the Small Intestine of Mice Following Salmonella Typhimurium Infection. Frontiers in Immunology, 10, 643.

+ Open protocol

+ Expand

Flow Cytometric Phenotyping of Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monocyte-derived macrophages or BMDMs were harvested and washed in cold PBS. Cells were incubated in Fc-block reagent (BD biosciences) and fixable viability dye eFluor 780 (Invitrogen) for 15 min at 4°C in cold PBS. Monocyte-derived macrophages were washed once and stained with the following antibodies: anti-CD62L PerCp-Cy5.5 (BD biosciences), anti-CCR2 APC (R and D Systems), anti-F4/80 efluor450 (eBioscience), anti-CSF1R BV711 (Biolegend), anti-Ly6G BUV395 (BD biosciences), anti-CD11b BUV737 (BD biosciences), anti-MHCII(I-A/I-E) PE (eBioscience) and anti-Ly6C PE-Cy7 (eBioscience). BMDMs were washed once and stained with the following antibodies: anti-CD45 FITC (eBioscience), anti-F4/80 BV421 (Biolegend), anti-CD11b BUV395 (BD biosciences). Stained cells were analyzed on by flow cytometry using a BD FACS CANTO instrument. Loss of eGFP or CD45 was assessed by gating on live F4/80 + macrophages using FlowJo X.

Lim J., Park H., Heisler J., Maculins T., Roose-Girma M., Xu M., Mckenzie B., van Lookeren Campagne M., Newton K, & Murthy A. (2019). Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. eLife, 8, e44452.

+ Open protocol

+ Expand

Profiling Myeloid Cells in Murine EAE

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood was collected in 2% EDTA tubes from the orbital vein of 16 isoflurane-anesthetized mice at disease onset (clinical score ≥ 0.5) and at the peak of the EAE. After blocking Fc receptors, cells were labeled with the following antibodies: anti-Ly-6C FITC (10 µg/mL, AL-21 clone), anti-Ly-6G PE (4 µg/ml, 1A8 clone), and anti-CD11b PerCP-Cy5.5 (4 µg/ml, M1/70 clone) all from BD Biosciences; anti-MHC-II PE-Cy7 (4 µg/ml, M5/114.15.2 clone), anti-CD11c APC (4 µg/ml, N418 clone), and anti-F4/80 eFluor450 (4 µg/ml, BM8 clone) from eBioscience. Analysis was performed in an FACS Canto II cytometer (BD Biosciences) and data analysis was assessed using FlowJo 10.6.2 software (FlowJo, LLC-BD Biosciences).

Ortega M.C., Lebrón-Galán R., Machín-Díaz I., Naughton M., Pérez-Molina I., García-Arocha J., Garcia-Dominguez J.M., Goicoechea-Briceño H., Vila-del Sol V., Quintanero-Casero V., García-Montero R., Galán V., Calahorra L., Camacho-Toledano C., Martínez-Ginés M.L., Fitzgerald D.C, & Clemente D. (2023). Central and peripheral myeloid-derived suppressor cell-like cells are closely related to the clinical severity of multiple sclerosis. Acta Neuropathologica, 146(2), 263-282.

+ Open protocol

+ Expand

Myeloid cell recruitment by cancer cell supernatants

Check if the same lab product or an alternative is used in the 5 most similar protocols

In vivo recruitment of myeloid cells by culture supernatants of treated HCT116 cells was determined in peritonitis assays as described previously with minor variations (47 (link)–49 (link)). In brief, conditioned supernatants were injected intraperitoneally (i.p.) into C57BL/6 mice, and peritoneal lavages were collected from sacrificed mice after 6 h (n = 4 per group) or after 6, 12, and 24 h (n = 2 per group) for time course analyses, respectively. Total numbers of leukocytes were determined by using a Coulter A C T counter (Beckman Coulter, Krefeld, Germany). Cells were stained on ice for 30 min with the following monoclonal antibodies: anti-CD45-APC-Cy7 (from BD Biosciences), anti-CD11b-FITC, anti-Gr-1-PE, anti-F4/80-eFluor450 (from eBiosciences, San Diego, CA, United States). FACS analyses were performed upon lysis of erythrocytes (Gallios, Beckman Coulter). Monocytic cells and granulocytes were distinguished and quantified within the CD45⁺/CD11b⁺ myeloid cell population by cell surface expression levels of Gr-1 and F4/80.

Ernst A., Hennel R., Krombach J., Kapfhammer H., Brix N., Zuchtriegel G., Uhl B., Reichel C.A., Frey B., Gaipl U.S., Winssinger N., Shirasawa S., Sasazuki T., Sperandio M., Belka C, & Lauber K. (2020). Priming of Anti-tumor Immune Mechanisms by Radiotherapy Is Augmented by Inhibition of Heat Shock Protein 90. Frontiers in Oncology, 10, 1668.

+ Open protocol

+ Expand

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Collagenase Type II digested tissues were passed through a 40μm cell strainer. Peripheral blood was prepared by lysing one drop of tail blood for twenty minutes with RBC lysis buffer. Single-cell suspensions were stained with eFluor780- anti-CD45 (eBioscience), PE-anti-Ly6g (BD), APC-anti-Cd11b (eBioscience), FITC-anti-B220 (eBioscience) and eFluor450 anti-F4/80 (eBioscience). Flow cytometry was performed using an LSRFortessa (BD). Flow cytometry data was analyzed using FlowJo software (BD Biosciences).

Triner D., Devenport S.N., Ramakrishnan S.K., Ma X., Frieler R.A., Greenson J.K., Inohara N., Nunez G., Colacino J.A., Mortensen R.M, & Shah Y.M. (2018). Neutrophils Restrict Tumor-Associated Microbiota to Reduce Growth and Invasion of Colon Tumors in Mice. Gastroenterology, 156(5), 1467-1482.

+ Open protocol

+ Expand

Analysis of Cytokine Secretion and Intracellular Expression in Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For cytokine secretion, 5×10⁴ cells were plated per well of 96-well plates in 200 μl complete media and allowed to adhere 3 hours - overnight. TLR stimuli were added to the wells and after 16 hours the levels of TNF, IL6,IL12 p40 and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA Ready-SET-Go eBioscience). For intracellular cytokine staining, 1×10⁵ cells were plated per well of 48-well non-TC treated plates and stimulated in the presence of the protein transport inhibitor BD GolgiStop™ (BD Biosciences) for 6 h. For IL-10 neutralization experiments, cells were pre-treated with indicated dilutions anti-IL-10 (clone JES5-2A5, eBioscience) or 1000 ng/ml rat IgG2b isotype control (eBioscience) for 30 minutes prior to addition of stimuli. After stimulation, cells were lifted using enzyme-free Hank's cell dissociation buffer (Life Technologies), blocked with anti-CD16/CD32 (BD Biosciences), fixed, permeabilized and stained with eFluor450 anti-F4/80 (eBioscience), FITC anti-TNF alpha (eBioscience) and PE anti-IL6 antibodies (eBioscience). Apoptotic cells were identified by staining with annexin V and 7-amino-actinomycin D (BD Biosciences). For each experiment, cells were analyzed by flow cytometry using a BD LSR II running FACSDiva software (BD) and with FlowJo (TreeStar).

Tampella G., Kerns H.M., Niu D., Singh S., Khim S., Bosch K.A., Garrett M.E., Moguche A., Evans E., Browning B., Jahan T.A., Nacht M., Wolf-Yadlin A., Plebani A., Hamerman J.A., Rawlings D.J, & James R.G. (2015). The Tec kinase-regulated phosphoproteome reveals a mechanism for the regulation of inhibitory signals in murine macrophages. Journal of immunology (Baltimore, Md. : 1950), 195(1), 246-256.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!