Cells were stained for PE-conjugated anti-human PD-L2 (Cat#329606, Biolegend) or APC-conjugated anti-human PD-L1 antibody (Cat#329708, Biolegend) at 1:100 dilution on ice for 30 mins and analyzed by BD FACSCalibur. Isotype controls are mouse IgG2a, κ (Cat#400213, Biolegend) and mouse IgG2b, κ (Cat#400322, Biolegend), respectively. GL261 cells overexpressing PD-L1 and/or PD-L2 were stained for PE-conjugated anti-mouse PD-L2 (Cat#107205, Biolegend) and/or APC-conjugated anti-mouse PD-L1 antibody (Cat#124312, Biolegend) at 1:100 dilution on ice for 30 minutes and sorted using a BD FACSAria III cell sorter. Isotype controls were Rat IgG2a, κ (Cat#400508, Biolegend) and Rat IgG2b, κ (Cat#400612, Biolegend), respectively.
Apc conjugated anti human pd l1 antibody
Manufactured by BioLegend
Sourced in United States
The APC-conjugated anti-human PD-L1 antibody is a flow cytometry reagent used for the detection and analysis of PD-L1 expression on human cells. This antibody is conjugated to the fluorescent dye allophycocyanin (APC), which can be excited by a red laser and detected in the APC channel.
Automatically generated - may contain errors
Lab products found in correlation
Cell staining buffer, by BioLegend (2 mentions)
Rat igg2b, by BioLegend (1 mentions)
Mouse igg2a, by BioLegend (1 mentions)
Mouse igg2b, by BioLegend (1 mentions)
Rat igg2a, by BioLegend (1 mentions)
Facscalibur, by BD (1 mentions)
Facsaria 3 cell sorter, by BD (1 mentions)
Fluorescence activated cell sorting (facs), by BD (1 mentions)
Apc mouse igg2b, by BioLegend (1 mentions)
Facscanto 2 cytometer, by BD (1 mentions)
6 protocols using apc conjugated anti human pd l1 antibody
1
PD-L1 and PD-L2 Expression Analysis
2
Cell Surface PD-L1 Detection Protocol
3
PD-L1 Expression via Flow Cytometry
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Flow cytometry assays were performed for cell surface PD-L1 detection. After harvested with trypsin, cells were stained with APC- conjugated anti-human PD-L1 antibody (1:200) (BioLegend) for 30min at 4℃ followed by washing with PBS twice. The staining process was protected from light. And then cells were analyzed with flow cytometry. The above experiments were conducted independently three times.
4
PD-L1 Expression Analysis by Flow Cytometry
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After cells were trypsinized, 1 × 105 cells were re-suspended in 100 mL of staining buffer containing 1μl APC-conjugated anti-human PD-L1 antibody (BioLegend) and incubated at room temperature for 15 min. After washing with PBS for 3 times, cells were analyzed by flow cytometry.
5
Quantifying PD-L1 Expression in Cells
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After transfection with siRNA or induction with IFN-γ for the indicated time, cells were trypsinized and collected into different centrifuge tubes and washed with PBS twice. The cells were then incubated with allophycocyanin (APC)-conjugated anti-human PD-L1 antibody (1:250) (BioLegend, San Diego, CA, USA) for 30 min on ice. After incubation, the stained cells were washed twice with cold PBS and analyzed by FACS. All staining steps were protected from light. The gating strategy used for flow cytometry is shown in Supplementary Fig. 9b .
6
PD-L1 Expression Analysis by Flow Cytometry
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