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2 protocols using fecl2 4h2o

1

Synthesis and Characterization of Inorganic Nanoparticles

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Inositol hexakisphosphate aqueous solution (IP6, 90%) was purchased from Sigma-Aldrich. FeCl3·6H2O (99%), FeCl2·4H2O (99%), NaOH (≥96.0%), sodium citrate (Na3C6H5O7·2H2O, 99.8%), and rhodamine 6G (R6G) were from Adamas Reagent. Cyclohexane (CYH, 99.5%) and absolute alcohol (CH3CH2OH) were obtained from Macklin Reagent (Shanghai, China). Chloroauric acid (HAuCl4·4H2O, 99.9%) was bought from Sinopharm Chemical Reagent (Shanghai, China). MDA was provided by Zhejiang Police College, Hangzhou. All reagents were used without further purification. All solutions were made with ultrapure water (18.25 MΩ cm), which was produced by using a Millipore water purification system.
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2

Fabrication and Characterization of Heavy Metal Nanoparticles

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Chicken eggs were bought from the local market. K2SO4, AgNO3, SnCl2·4H2O, BaCl2, Pb(NO3)2, CuSO4·5H2O, MgSO4·7H2O, MnSO4·H2O, FeCl2·4H2O, FeCl3, ZnCl2, NaCl,CaCl2, L-alanine, L-lysine, L-phenylalanine, L-cysteine, L-arginine, L-glycine, L-aspartic acid, L-glutamine, glucose, fructose, urea and glutathione (GSH) were purchased from Adamas-Beta Co., Ltd. (Shanghai, China). Dopamine hydrochloride and tris(hydroxymethyl)aminomethane (Tris) were obtained from 9 Ding Chemistry Co., Ltd. (Shanghai, China). 96-well plates (In vitro scientific) were obtained from Xinyou Biotechnology Co., Ltd. (Hangzhou, China). 6-well plates were obtained from Nest Biotechnology Co., Ltd. (Wuxi, China). Black opaque 96-well plates were supported by Jet Bio-Filtration Co., Ltd. (Guangzhou, China). Dulbecco’s modified Eagle’s medium (DMEM), trypsin, penicillin, streptomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Sijiqing Biotechnology Co., Ltd., (Hangzhou, China). All of the chemicals were analytical grade and used without further purification.
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