Polyacrylamide gels were made on (3-Aminopropyl) trimethoxysilane (APTMS) (Alfa Aesar, Haverhill, MA, USA) and glutaraldehyde (Amresco, Cleveland, OH, USA) treated 25 µm × 25 µm glass slides with acrylamide (Bio-Rad, Hercules, CA, USA), bis-acrylamide (Bio-Rad), 8.2 pH HEPES buffer (Amresco), ammonium persulfate (APS) (Amresco), and tetramethylethylenediamine (TEMED) (Amresco) as previously described [45 ]. Treated glass slides were UV-glued into culture dishes prior to gel preparation. Three volumes of acrylamide/bis-acrylamide (5%/0.04%, 5%/0.08%, 8%/0.10%) were used to obtain three surface stiffness conditions of 1, 2, and 7.5 kPa respectively. The gels were coated with 0.1 mg/mL type I rat tail collagen after the gels were UV treated with 1 mg/mL sulfo-SANPAH (ProteoChem, Loves Park, IL, USA). Similarly, glass slides were coated with 0.1 mg/mL type I rat tail collagen after being incubated at room temperature in 0.1 mg/mL Poly-l -Lysine. Cells were seeded onto the surfaces at 10% confluency prior to measurement.
Ammonium persulfate aps
Manufactured by Avantor
Sourced in United States
Ammonium persulfate (APS) is a chemical compound commonly used as an oxidizing agent and initiator in various applications. It is a white crystalline solid that is soluble in water. APS is commonly used in the production of polymers, detergents, and as a component in some analytical and life science applications.
Automatically generated - may contain errors
Lab products found in correlation
Bis acrylamide, by Bio-Rad (1 mentions)
Glutaraldehyde, by Avantor (1 mentions)
Acrylamide, by Bio-Rad (1 mentions)
Sodium hydroxide (naoh), by Avantor (1 mentions)
Methanol, by Avantor (1 mentions)
3 trimethoxysilyl propyl methacrylate, by Merck Group (1 mentions)
Acrylamide, by Avantor (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
Hydrochloric acid (hcl), by Avantor (1 mentions)
Chloroform, by Avantor (1 mentions)
Acetone, by Avantor (1 mentions)
Toluene, by Avantor (1 mentions)
Triethylamine tea, by Avantor (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Polystyrene beads, by Polysciences (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Xylene, by Thermo Fisher Scientific (1 mentions)
Millex lh filter, by Merck Group (1 mentions)
Ethanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
6 protocols using ammonium persulfate aps
1
Polyacrylamide Gels for Cell Culture
2
Capillary Electrophoresis Protein Separation
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Methanol, sodium hydroxide and acrylamide were purchased from VWR (Radnor, PA, USA). Ammonium persulfate (APS) was from AMRESCO (Solon OH, USA) and N,N,N',N'tetramethyl-ethylendiamine (TEMED) was from Alfa Aesar (Karlsruhe, Germany). The 3-(trimethoxysilyl)propyl methacrylate and all other chemicals were purchased from Sigma Aldrich (St. Louis, MO, USA). The Protein Methods Development kit containing the pH 3 Citrate Buffer (50 mM), pH 6 Citrate/MES buffer (50 mM), as well as the Protein Test Mixture of lysozyme, ribonuclease A and cytochrome C was from SCIEX (Brea, CA, USA). The 75 μm ID / 375 μm OD bare fused silica capillary tubing was from Polymicro Technologies (Phoenix, AZ, USA)
3
Synthesis of CNC-Based Polymer Composites
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The CNC suspension used in this paper had a solid content of 10.4% (w/w) and was purchased from the University of Maine (Maine, USA), which was prepared using the sulfuric acid method. Acetone (>99%), chloroform (>99%), toluene (>99%), dimethylsulfoxide (DMSO) (>99.9%), triethylamine (TEA) (>99%), ammonium persulfate (APS) (>98%), and hydrochloric acid (37%) were all purchased from VWR (Darmstadt, Germany) whereas 2,4-toluene diisocyanate (2,4-TDI) (>98%) was purchased from TCI Chemicals (Eschborn, Germany) and was stored in the fridge in sealed bottles. DMSO, TEA, and toluene were stored over an A4 molecular sieve, and Acetone was stored over an A3 molecular sieve. Both molecular sieves were purchased from Carl Roth (Karlsruhe, Germany) and regenerated before use.
4
Fabrication of Ag/AgCl Electrodes and Polystyrene Beads
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The Ag/AgCl electrodes were fabricated by chloriding 0.375 mm Ag wires (Warner Instruments, Hamden, USA) in a 1 M KCl solution. Polystyrene beads were purchased from Polyscience. Poly(ethylene glycol) diacrylate (PEGDA, MW 700 Da) mineral oil was purchased from Sigma-Aldrich. Ammonium persulfate (APS) was purchased from VWR. Phosphate buffered saline (PBS) (1 ×, pH 7.4) with 0.05% Tween-20 was purchased from TEKnova. Triton X-100 was purchased from EMD Millipore.
5
N-Glycan Release and Liquid Chromatography
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For N-glycan release and analysis by liquid chromatography, the following materials and reagents were used. AcroPrep Advance 96-filter plates and 10 kDa MWCO microcentrifuge filtration tubes were acquired from Pall Life sciences (USA). Polypropylene 2 mL deep 96-well blocks were purchased from Fisher Scientific (Ireland) and 0.45 μm Millex-LH filters from Millipore (Ireland). Plate seals were obtained from Cruinn (Ireland) and silicone sealing mats from Phenomenex (Germany). Protogel was purchased from National Diagnostics (UK) and ammonium hydroxide solution was acquired from Fluka (Ireland). Formic acid and ammonium persulfate (APS) were obtained from VWR chemicals (Ireland). Ultrapure water was filtered through an arium® ProUV system (Sartorius, Germany). HPLC grade methanol, ethanol, acetonitrile, xylene and water were acquired from Fisher Scientific (Ireland). PNGase F was purchased from New England Biolabs. All other reagents were purchased from Sigma (including N,N,N′,N′-tetramethylethane-1,2-diamine (TEMED), iodoacetamide (IAA), dithiothreitol (DTT) and 2-aminobenzamide (2-AB)) unless otherwise specified.
6
Graphene Sensor Fabrication Protocol
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The SLG coated with PMMA was purchased from Graphenea Inc. To fabricate the sensor, the PMMA/SLG/Cu samples were cut into 1 cm 1 cm squares and then placed into 200 mL 0.1 M Ammonium persulfate (APS, purchased from VWR) to etch off the copper film. After about 30 min, the PMMA/SLG samples were transferred to 500 mL DI water to rinse off the remaining APS and transferred on a glass slide (VWR). The samples were first heated to 100 °C for 30 min and then annealed for 2 h at 600 °C under N2 using a three-heat zone tube furnace (Lindberg Thermodyne 21100). The samples were then immersed in acetone overnight (16 h) to remove the PMMA. The annealing step is essential to achieve minimum PMMA residue on graphene.
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