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Tecnai g2 spirit 120 kv electron

Manufactured by Thermo Fisher Scientific
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The Tecnai G2 Spirit 120 KV electron microscope is a high-performance transmission electron microscope designed for advanced materials characterization. It operates at an accelerating voltage of 120 kilovolts and provides detailed imaging and analytical capabilities for a wide range of specimens.

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Lab products found in correlation

Copper grid, by Ted Pella (3 mentions)

3 protocols using tecnai g2 spirit 120 kv electron

1

Negative Staining of MAVS Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Copper grids (Ted Pella Inc.) coated with a layer of thin carbon film (5-10 nm) were rendered hydrophilic by glow-discharge in air. Seven microliters of purified MAVS samples were loaded onto the grids, incubated for 3 min, and stained with 2% uranyl acetate. After air drying for a few minutes, the grids were examined under a FEI Tecnai G2 Spirit 120 KV electron microscope. Images were taken in low-dose mode (20 electron/A˚2) at 67,000 x with defocus levels varying between 0.5 to 1.2 microns. Regular calibrations of the microscope magnifications found that the nominal 67,000x used here was always accurate within 3.0% error.
Li J., Zhang R., Wang C., Zhu J., Ren M., Jiang Y., Hou X., Du Y., Wu Q., Qi S., Li L., Chen S., Yang H, & Hou F. (2023). WDR77 inhibits prion-like aggregation of MAVS to limit antiviral innate immune response. Nature Communications, 14, 4824.
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2

Negative Staining of MAVS Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Copper grids (Ted Pella Inc.) coated with a layer of thin carbon film (5-10 nm) were rendered hydrophilic by glow-discharge in air. Seven microliters of purified MAVS samples were loaded onto the grids, incubated for 3 min, and stained with 2% uranyl acetate. After air drying for a few minutes, the grids were examined under a FEI Tecnai G2 Spirit 120 KV electron microscope. Images were taken in low-dose mode (20 electron/A˚2) at 67,000 x with defocus levels varying between 0.5 to 1.2 microns. Regular calibrations of the microscope magnifications found that the nominal 67,000x used here was always accurate within 3.0% error.
Li J., Zhang R., Wang C., Zhu J., Ren M., Jiang Y., Hou X., Du Y., Wu Q., Qi S., Li L., Chen S., Yang H, & Hou F. (2023). WDR77 inhibits prion-like aggregation of MAVS to limit antiviral innate immune response. Nature Communications, 14, 4824.
+ Open protocol
+ Expand
3

Negative Staining of MAVS Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Copper grids (Ted Pella Inc.) coated with a layer of thin carbon film (5-10 nm) were rendered hydrophilic by glow-discharge in air. Seven microliters of purified MAVS samples were loaded onto the grids, incubated for 3 min, and stained with 2% uranyl acetate. After air drying for a few minutes, the grids were examined under a FEI Tecnai G2 Spirit 120 KV electron microscope. Images were taken in low-dose mode (20 electron/A˚2) at 67,000 x with defocus levels varying between 0.5 to 1.2 microns. Regular calibrations of the microscope magnifications found that the nominal 67,000x used here was always accurate within 3.0% error.
Li J., Zhang R., Wang C., Zhu J., Ren M., Jiang Y., Hou X., Du Y., Wu Q., Qi S., Li L., Chen S., Yang H, & Hou F. (2023). WDR77 inhibits prion-like aggregation of MAVS to limit antiviral innate immune response. Nature Communications, 14, 4824.
+ Open protocol
+ Expand

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