MA104 cells (ATCC) were cultured in M199 media (Invitrogen) supplemented with 25 mM HEPES and 10% HI-FBS (Invitrogen). BSC-1 cells (ATCC) were cultured in DMEM (Invitrogen) supplemented with 10% HI-FBS (Invitrogen). For VP4 and VP7 expression, full-length genomic sequences from rhesus rotavirus (G3 serotype, NCBI:txid444185) were amplified by PCR and cloned into pFastbac (Thermo Fisher Scientific) expression vector. Mutations were introduced by quick-change mutagenesis in DH10α cells (Thermo Fisher Scientific). Purified plasmid constructs were transfected into DH10-Bac cells (Thermo Fisher Scientific). Purified bacmids were transfected into SF9 cells (ATCC) grown in SF900 II SFM media supplemented with 1% pen-strep.
Dh10α cells
Manufactured by Thermo Fisher Scientific
DH10α cells are competent Escherichia coli (E. coli) cells commonly used in molecular biology applications. They are designed for efficient transformation and propagation of plasmid DNA. DH10α cells exhibit high transformation efficiency and are suitable for a variety of cloning and amplification procedures.
Automatically generated - may contain errors
Lab products found in correlation
Hi fbs, by Thermo Fisher Scientific (2 mentions)
Dh10bac cells, by Thermo Fisher Scientific (2 mentions)
Sf9 cells, by American Type Culture Collection (2 mentions)
M199 media, by Thermo Fisher Scientific (2 mentions)
Pfastbac, by Thermo Fisher Scientific (2 mentions)
Bsc 1 cells, by American Type Culture Collection (2 mentions)
Ma104 cells, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
2 protocols using dh10α cells
1
Recombinant Rotavirus Protein Expression
2
Recombinant Rotavirus Protein Expression
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MA104 cells (ATCC) were cultured in M199 media (Invitrogen) supplemented with 25 mM HEPES and 10% HI-FBS (Invitrogen). BSC-1 cells (ATCC) were cultured in DMEM (Invitrogen) supplemented with 10% HI-FBS (Invitrogen). For VP4 and VP7 expression, full-length genomic sequences from rhesus rotavirus (G3 serotype, NCBI:txid444185) were amplified by PCR and cloned into pFastbac (Thermo Fisher Scientific) expression vector. Mutations were introduced by quick-change mutagenesis in DH10α cells (Thermo Fisher Scientific). Purified plasmid constructs were transfected into DH10-Bac cells (Thermo Fisher Scientific). Purified bacmids were transfected into SF9 cells (ATCC) grown in SF900 II SFM media supplemented with 1% pen-strep.
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