Criterion tank blotter

Manufactured by Bio-Rad

The Criterion Tank Blotter is a lab equipment used for protein transfer and blotting. It is designed to facilitate the transfer of proteins from polyacrylamide gels to membranes for further analysis and detection.

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Lab products found in correlation

Tris tricine precast gel, by Bio-Rad (2 mentions) 494 procise protein sequencer 140c analyzer, by Thermo Fisher Scientific (2 mentions) Precision plus protein prestained standards, by Bio-Rad (1 mentions)

Spelling variants of this product

Criterion Blotter (69 citations) Tank blotter (7 citations) Criterion blotter transfer tank (2 citations)

2 protocols using criterion tank blotter

Protein Sequencing Protocol

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Ni-NTA purified L27 Δ 2–9 His6 protein samples were loaded onto a 16.5% Tris-tricine precast gel (Biorad). The protein was electroblotted to a 0.45 μm PVDF membrane for 4 hours at 300 mA in a Criterion Tank Blotter (Biorad). The membrane was stained with Coomassie and the appropriate band was marked, coded and sent to the Iowa State University Protein Facility. There the protein samples on the membrane were washed six times with deionized water and loaded onto a 494 Procise Protein Sequencer/140C Analyzer (Applied Biosystems, Inc).

Wall E.A., Johnson A.L., Peterson D.L, & Christie G.E. (2017). Structural Modeling and Functional Analysis of the Essential Ribosomal Processing Protease Prp from Staphylococcus aureus. Molecular microbiology, 104(3), 520-532.

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Ni-NTA Purified Protein Sequencing

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Ni-NTA purified L27 Myc-His₆ protein samples were loaded onto a 16.5% Tris-tricine precast gel (Biorad) and run until the 10kDa band of the Precision Plus Protein Prestained Standards (Biorad) approached the bottom of the gel. These proteins were electroblotted to a 0.45 μm PVDF membrane for 4 hours at 300mA in a Criterion Tank Blotter (Biorad). The membrane was then stained with Coomassie and the appropriate bands were marked and coded and sent to the Iowa State University Protein Facility for Edman degradation. There the protein samples on the membrane were washed six times with deionized water and loaded onto a 494 Procise Protein Sequencer/140C Analyzer (Applied Biosystems, Inc).

Wall E.A., Caufield J.H., Lyons C.E., Manning K.A., Dokland T, & Christie G.E. (2014). Specific N-terminal cleavage of ribosomal protein L27 in Staphylococcus aureus and related bacteria. Molecular microbiology, 95(2), 258-269.

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