Nod cg prkdcscid il2rgtm1wjl szj female mice

KE-39 and CAT12 cells were prepared in a 1:1 ratio of Matrigel to medium and 3 × 10⁶ CAT12 and 5 × 10⁶ KE-39 cells were prepared in a 1:1 ratio of Matrigel to medium (Matrigel; Corning) and injected subcutaneously into the flanks of NOD.Cg-Prkdc^scid IL2rg^tm1Wjl/SzJ female mice (6–8 weeks old) purchased from Jackson Laboratory. Treatment was initiated when tumors reached 100–150 mm³. Electronic calipers were used for tumor measurements twice a week and tumor volumes were calculated using the following formula: volume = length × width² × 0.5. Mice were sacrificed when tumors reached the endpoint. Dissected tumors were snap-frozen and stored at –80°C or were fixed in 10% buffered formalin for histopathologic process.