Deltavision omx blaze microscope

Synaptic arbors of muscle 6/7 in the abdominal segment A2 were used for all analyses. Imaging was performed on a Nikon A1R point scanning confocal and a Nikon Yokogawa spinning disc confocal with a Hamamatsu ORCA‐R2 cooled CCD camera. 3D‐SIM was performed on a DeltaVision OMX Blaze microscope (GE Healthcare Life Sciences, Marlborough, MA, USA) with a PCO sCMOS camera. Lasers were adjusted to prevent oversaturation. Images were processed and analyzed with ImageJ and/or MATLAB. Bouton number and size were counted and traced by hand. An HRP mask was used to restrict analysis to neuronal signal for intensity analysis, and MATLAB scripts were used to quantify dTACC and tubulin signals relative to HRP. Line scans were used to create intensity profiles to distinguish different Futsch structures.

Structured illumination (3D-SIM) was performed using a DeltaVision OMX Blaze microscope (GE Healthcare) coupled to a Photometrics Evolve 512 EMCCD camera (Photometrics) and a PlanAPO 60× oil-immersion objective (NA = 1.4) for an effective pixel size of 80 and 40 nm after reconstruction (Olympus).