Au400e

Urine samples were shipped on dry ice to the NIEHS biorepository, where they were stored at −80 °C. We shipped frozen urine samples to the CDC in three batches. For women in the case-cohort study who did not develop UL during follow-up, we measured urinary concentrations in samples collected at baseline, 20 months, and 40 months. For incident UL cases detected during follow-up, we measured urinary concentrations in samples collected from baseline until the time point before UL detection. Urinary concentrations of BPA, BPF, and BPS; 2,4- and 2,5-dichlorophenol; benzophenone-3; triclocarban; triclosan; and methylparaben, ethylparaben, propylparaben, and butylparaben were quantified at the CDC using methods based on online solid-phase extraction coupled to high performance liquid chromatography-isotope dilution mass spectrometry (28 , 29 ). We followed strict Clinical Laboratory Improvement Amendments guidelines, including quality control materials and reagent blanks in each analytic run. Inter-batch precision varied by analyte and concentration, but was generally <7% (30 ). We measured urinary creatinine concentrations at NIEHS using a Beckman Coulter clinical analyzer AU400e.

Blood serum samples (approximately 50ul) from the female CD-1 offspring at PND 91 necropsies were collected for evaluation. The serum chemistry analyses (alkaline phosphatase, ALP; alanine aminotransferase, ALT; aspartate aminotransferase, AST; glucose, triglyceride, low-density lipoproteins, LDL; high-density lipoproteins, HDL, total bile acid, TBA; sorbitol dehydrogenase, SDH; and cholesterol) were determined using an Olympus AU400e (Beckman Coulter, Inc., Irving, TX) and reagents obtained from the instrument manufacturer (except LDL, HDL, and TBA – reagents obtained from Sekisui Diagnostics, LLC., Framingham, MA). Leptin and insulin immunoassays were performed in duplicate using a Sector Imager 2400 (Meso Scale Discovery, Gaithersburg, MD) and multiplexed reagents obtained from the instrument manufacturer. Assays were carried out as directed by the manufacturer.