Atomic force microscopy (Dimension Icon & Fast-Scan Bio AFM from Bruker, Germany) was used for observing and measuring the PNR samples. Commercial AFM tips (Olympus Micro Cantilevers Company, Model: OMCL-AC240TS-R3) were used. The AFM tip has a tip radius of 7 nm, a spring constant of 1.7 N m−1, and a resonant frequency of 70 kHz. To obtain a more accurate width, the same AFM tips were used to measure the apparent widths and heights of the CVD-grown multiwalled carbon nanotubes. These have similar heights as the measured PNRs to deduce the effect of the AFM tip size on the PNR width. According to our measurement, the actual width of the PNR was about 14 nm less than the measured apparent width when the effect of the AFM tip size was corrected.
Fast scan bio afm
Manufactured by Bruker
Sourced in United States, Germany
The Fast-Scan Bio AFM is a high-speed atomic force microscope designed for biological applications. It enables the capture of dynamic processes at the nanoscale in real-time.
Automatically generated - may contain errors
Lab products found in correlation
Dimension icon, by Bruker (2 mentions)
H 7500 transmission electron microscope, by Bruker (2 mentions)
Silicon afm tips, by Bruker (2 mentions)
Zetasizer nano zs90, by Malvern Panalytical (2 mentions)
Nanoscope iiia controller, by Digital Instruments (2 mentions)
Multimode afm, by Digital Instruments (2 mentions)
Tr400psa silicon nitride probes, by Olympus (2 mentions)
Fastscan d probes, by Bruker (2 mentions)
Scanasyst fluid probe, by Bruker (1 mentions)
Poly l lysine (pll), by Solarbio (1 mentions)
0.22 m filter, by Merck Group (1 mentions)
Dimension fastscan afm, by Bruker (1 mentions)
Nanoscope analysis 1, by Bruker (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Nch vs1 w, by NanoWorld (1 mentions)
Multimode quadrax mm scanning probe microscope, by Bruker (1 mentions)
Fastscan a cantilever, by Bruker (1 mentions)
Nanoscope software, by Veeco (1 mentions)
11 protocols using fast scan bio afm
1
Atomic Force Microscopy of PNRs
2
Atomic Force Microscopy Reveals Rifabutin's Impact on Mycobacterial Membranes
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Atomic force microscopy was
used to examine the influence of Rifabutin on the topography of mycobacterial
membranes.58 (link) The liposomes were prepared
as in the SPR experiment and diluted to 0.5 mg/mL with the addition
of 4 mM CaCl2. To freshly peeled mica, 200 μL of
0.5 mg/mL liposomal solution was added and kept at RT overnight in
a clean humid environment to form SLBs. The unbound lipids were washed
out, and fresh HBS buffer was added. The topography of the SLBs was
characterized using PeakForce Mapping in Fluid in Nanomechanical Mapping
mode on a FastScan Bio AFM (Bruker AXS, CA) controlled with Nanoscope
9.7 software. A triangular ScanAsyst-Fluid+ probe (Bruker, CA) with
a nominal tip of 2 nm and a nominal spring constant of 0.7 N/m was
used, and imaging was performed in the fluid condition. The SLBs formed
on the mica were scanned with a droplet method, wherein the probe
loaded onto the scanner was prewet with 30 μL of HBS followed
by engaging the sample. The force setpoint was manually maintained
in between 600 and 900 pN with the feedback gain automatically adjusted
by software. The amplitude and frequency of peak force were set at
70–80 nm and 2 kHz, respectively. The topographic images were
analyzed with NanoScope Analysis software and processed using Gwyddion
2.56 software.
used to examine the influence of Rifabutin on the topography of mycobacterial
membranes.58 (link) The liposomes were prepared
as in the SPR experiment and diluted to 0.5 mg/mL with the addition
of 4 mM CaCl2. To freshly peeled mica, 200 μL of
0.5 mg/mL liposomal solution was added and kept at RT overnight in
a clean humid environment to form SLBs. The unbound lipids were washed
out, and fresh HBS buffer was added. The topography of the SLBs was
characterized using PeakForce Mapping in Fluid in Nanomechanical Mapping
mode on a FastScan Bio AFM (Bruker AXS, CA) controlled with Nanoscope
9.7 software. A triangular ScanAsyst-Fluid+ probe (Bruker, CA) with
a nominal tip of 2 nm and a nominal spring constant of 0.7 N/m was
used, and imaging was performed in the fluid condition. The SLBs formed
on the mica were scanned with a droplet method, wherein the probe
loaded onto the scanner was prewet with 30 μL of HBS followed
by engaging the sample. The force setpoint was manually maintained
in between 600 and 900 pN with the feedback gain automatically adjusted
by software. The amplitude and frequency of peak force were set at
70–80 nm and 2 kHz, respectively. The topographic images were
analyzed with NanoScope Analysis software and processed using Gwyddion
2.56 software.
3
Multimodal Nanoparticle Characterization
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Temperature controlled dynamic light scattering (DLS) was performed on a Zetasizer Nano ZS90 (Malvern Instruments, Malvern UK). Negative staining transmission electron microscopy(TEM) was carried out on a Hitachi H-7500 transmission electron microscope at an accelerating voltage of 75 kV. AFM measurements were performed on a “FastScan Bio” AFM (Bruker, USA) in a closed liquid cell with the ability to control the temperature of the solution from room temperature to 55 °C. Silicon AFM tips (Bruker)with a tip radius of curvature = 12 nm were used to image the sample in peak-force tapping mode.
4
Atomic Force Microscopy of Samples
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The AFM measurements of the samples were conducted using a Dimension Icon and Fast-Scan Bio AFM from Bruker, Germany. The commercial AFM tips (Model: OMCL-AC240TS-R3, Olympus Micro Cantilevers Company, Tokyo, Japan) were utilized in the measurements. These AFM tips have the following specifications: tip radius of 7 nm, spring constant of 1.7 N m−1, and resonant frequency of 70 kHz.
5
Atomic Force Microscopy Imaging of Biomolecules
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1-5 μl of transcription product was mixed with 40 μl AFM dilution buffer (12.5 mM Mg(OAc)2, 40mM KCl, 40mM NaCl, Tris-Borate pH 7.8) directly on the surface of a freshly-cleaved mica puck. Mixing is performed by vigorously pumping a 200 μl pipette tip ten times, before removing and discarding the fluid. The mica was washed with a solution of 60 mM NiCl2. Most AFM images were collected using a Multimode AFM (Digital Instruments) with a Nanoscope IIIA controller and a J-scanner. Olympus TR400PSA silicon nitride probes with a spring constant of ~.08 N/m were used for imaging, with a drive frequency of ~6-9 kHz. AFM in Supplementary Figs. 10 and 11 were collected with a Bruker Fastscan Bio AFM (Bruker) under buffer using FastScan-D probes (Bruker).
6
Multimodal Nanoparticle Characterization
7
AFM Imaging and Characterization of Extracellular Vesicles
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The freshly cleaved mica was cleaned with 3% hydrochloric acid with 96% ethanol for 20 times. Afterward, they were coated for 1 h in a 0.001% poly‐l ‐lysine (Solarbio) solution, rinsed with Milli‐Q water (18.2 MΩ.cm), and dried overnight at room temperature. The poly‐l ‐lysine modified mica was stored at 4 °C for no more than one month before use. The solution of sEVs was diluted ten times with PBS, filtered with a 0.22 µm filter (Millipore, USA), deposited on the poly‐l ‐lysine modified mica for absorbing sEVs and gently rinsed with PBS before imaging. AFM images were acquired in liquid under PeakForce QNM mode on Dimension FastScan AFM (Bruker, Billerica, CA) under the bio model (also called FastScan Bio AFM) using MLCT‐A probes (silicon nitride tip, nominal force constants 0.07 N m−1, nominal radius 20 nm, resonant frequency 15–30 kHz, Bruker, US). The imaging force was preset to be 0.5–3 nN. The force‐indentation curves were obtained at an indentation velocity of 250 nm s−1 until the surface was reached. The force‐indentation curves of the AFM tip approaching the mica surface were checked before and after each imaging of sEVs to make sure no contaminations from the sEVs adhered on the tip.
8
Atomic-Force Microscopy of Distal Femurs
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Eight weeks of posts MF surgery, the mice were euthanized and distal femurs were dissected. Utilizing a microtome blade to bisect the defect region. Half of the tissue was immediately fixed in 4% PFA and then embedded in paraffin. The other half of the fresh tissue was dissected further and cleaned. The tissue was then fixed to a plate utilizing tissue glue, and the plate was filled with protease-free PBS. The samples were imaged on a Bruker FastScan Bio-AFM. We used a pre-calibrated set of Nanotools 0.10 N/m 12 μm MLCT-O10 biosphere spherical probes. All variables were kept constant throughout experimental groups. The NanoScope Analysis 1.8 Software was used for analysis.
9
Bioink Characterization by Multifaceted Analysis
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The prepared bioinks were characterized by zeta potential measurements, atomic force microscopy (AFM) imaging and rheological analysis. The bioinks were diluted for 20‐fold by Milli‐Q water to zeta potential measurements on a Zetasizer Nano‐ZS (Malvern) at 25°C. For AFM imaging, 5 μL of bioink samples were deposited on mica sheets. After air‐dried, samples were observed using FASTSCANBIO AFM (Bruker) in a tapping mode, and the obtained images were processed using the nano‐scope analysis 1.9 software (Bruker). The rheological analysis including amplitude and frequency sweeps was performed on a MCR 302 rheometer (Anton Paar) equipped with a 25 mm diameter parallel plate at a 0.5 mm gap. The test temperature was maintained at 25 ± 3°C. The amplitude sweep was carried out in the strain range of 0.01%–100% with a fixed frequency of 1 Hz. The frequency sweep was performed in the range of 0.1–100 Hz at a constant shear strain of 1%.
10
Surface Morphology of Thin Films
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Surface morphology and roughness of the films on Si, glass, Cu, Ni and Al were determined on a Veeco Multimode Quadrax MM scanning probe microscope (Bruker, Billerica, MA, USA) using Si cantilevers (NCH-VS1-W from NanoWorld AG, Neuchatel, Switzerland) with a resonance frequency of 320 kHz and a force constant of 42 N·m−1. On the polymer substrates, the films were analyzed using a FastScanBio AFM using a FastScan-A cantilever (both from Bruker NANO) with typical resonance frequencies and force constants around 1.4 MHz and 18 N·m−1, respectively. All samples were analyzed in tapping mode in an ambient atmosphere at room temperature at the lowest possible force load and scan rates were adapted to obtain reliable surface data. Root mean square (RMS; Rq) roughness calculation and image processing was performed with the Nanoscope software (V7.30r1sr3, Veeco, Plainview, NY, USA).
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