Huangqi (Astragalus membranaceus) and Chenpi (Citri Reticulatae Blanco Pericarpium) were purchased from the First Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangzhou, China). Gegen (Pueraria lobata) was purchased from Beijing Tongrentang Anhui (Bozhou) Pieces (Beijing, China). Silymarin was purchased from Guangzhou Ruishu Biological Technology (Guangzhou, China). CCl4 was purchased from Guangzhou Chunchang Biotechnology Development (Guangzhou, China). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) assay kits were purchased from Beijing Jiuqiang Biotechnology (Beijing, China). Superoxide dismutase (SOD), malondialdehyde (MDA), reduced glutathione (GSH), and catalase (CAT) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China). Nrf2, Keap1, cytochrome P450 (CYP) 2E1, and HO-1 antibodies were purchased from Proteintech (Rosemont, IL, USA). Brusatol was purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA).
Catalase cat assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Catalase (CAT) assay kit is a laboratory instrument designed to measure the activity of the enzyme catalase. Catalase is an important antioxidant enzyme that helps protect cells from oxidative damage by breaking down hydrogen peroxide into water and oxygen. The assay kit provides a simple and reliable method for quantifying catalase levels in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Malondialdehyde mda assay kit, by Nanjing Jiancheng (11 mentions)
Peroxidase assay kit, by Nanjing Jiancheng (8 mentions)
Glutathione peroxidase gsh px assay kit, by Nanjing Jiancheng (8 mentions)
Superoxide dismutase sod assay kit, by Nanjing Jiancheng (7 mentions)
T sod assay kit, by Nanjing Jiancheng (5 mentions)
Total superoxide dismutase t sod assay kit, by Nanjing Jiancheng (3 mentions)
Hydrogen peroxide assay kit, by Nanjing Jiancheng (2 mentions)
Ascorbate peroxidase apx test kit, by Nanjing Jiancheng (2 mentions)
Mda assay kit, by Nanjing Jiancheng (2 mentions)
Proline assay kit, by Nanjing Jiancheng (2 mentions)
Reactive oxygen species assay kit, by Nanjing Jiancheng (2 mentions)
Myeloperoxidase assay kit, by Nanjing Jiancheng (2 mentions)
High speed refrigerated centrifuge, by Eppendorf (2 mentions)
Total superoxide dismutase sod assay kit, by Nanjing Jiancheng (2 mentions)
Keap1, by Proteintech (1 mentions)
Brusatol, by Merck Group (1 mentions)
Deae 52 cellulose, by Cytiva (1 mentions)
Losartan, by Merck Group (1 mentions)
Apocynin, by Merck Group (1 mentions)
Anti at1r, by Proteintech (1 mentions)
37 protocols using catalase cat assay kit
1
Astragalus and Citrus Peel Hepatoprotective Effects
2
Purification and Characterization of Dandelion Root Polysaccharides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dandelion root was purchased from a local market in Jilin, China. DEAE-52 cellulose was obtained from Whatman (Balston, UK). SephacrylTM S-200 was purchased from GE Healthcare Bio-Sciences AB (Uppsala, Sweden). 1-Phenyl-3-methyl-5-pyrazolone (PMP) was from Xiya Chemical Industry Co., Ltd (Shandong, China). L-Rhamnose, D-glucose and D-galactose were from Beijing Zhongke Biochemical Technology Co., Ltd. (Beijing, China). D-Mannose, L-arabinose and D-glucuronic acid were from the National Institutes for Food and Drug Control (Beijing, China). Dextran standards (4320, 12,600, 60,600, 110,000 and 289,000 Da) were purchased from Shanghai Macklin Biochemical Technology Co., Ltd (Shanghai, China). APAP was obtained from Sigma Chemical Co. (St. Louis, MO, USA). Aspartate aminotransferases (AST), malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), total superoxide dismutase (T-SOD) and catalase (CAT) assay kits were all obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Reactive oxygen species (ROS), Nrf2, Keap1, NQO1, HO-1 ELISA assay Kits were purchased from Nanjing Jin Yibai Biological Science and Technology Co., Ltd. (Nanjing, China).
3
Calcium Oxalate Crystals Induce Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Calcium oxalate monohydrate (COM) crystals were purchased from Macklin (Shanghai, China). COM crystals were weighed and suspended in sterile phosphate buffer solution at 1 mM and then were diluted into different concentrations. Ang II was purchased from Sangon Biotech (Shanghai, China). Anti-AT1R, anti-NADPH oxidase 2 (Nox2), anti-p50, and anti-p65 antibodies were purchased from Proteintech (Wuhan, China). Losartan and apocynin were purchased from Sigma Aldrich (St. Louis, MO, USA). 2′,7′-Dichlorofluorescein diacetate (DCF-DA) and Fluo-3AM were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Anti-NADPH oxidase 4 (Nox4), anti-OPN, and anti-MCP-1 antibodies were purchased from Abcam (Cambridge, MA, USA). An anti-CD44 antibody was purchased from Cell Signaling Technology (Boston, MA, USA). Malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China). An 8-OHdG assay kit was purchased from Elabscience (Wuhan, China). The transfection reagent Lipofectamine™ 2000 was purchased from Invitrogen (Carlsbad, CA, USA).
4
Rabbit Mitochondrial Dysfunction Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult New Zealand white rabbits (2.5–3.0 kg) were obtained from the Laboratory Animal Center of Anhui Medical University. All rabbits were kept in standard laboratory conditions with free access to rabbit chow and tap water. All experimental procedures were performed in compliance with the NIH guidelines for the use of experimental animals and approved by the Ethics Review Committee of Anhui Medical University. The mitochondrial isolation kit, malondialdehyde (MDA) assay kit, 8-iso-prostaglandin F2α (8-iso-PGF2α) assay kit, superoxide dismutase (SOD) assay kit and catalase (CAT) assay kit was purchased from Jiancheng Biotech Company (Nanjing, Jiangsu, China). The anti-SK/KCa1 (ab66624), anti-COX IV (ab66739), and anti-Tubulin (ab56676) antibodies were obtained from abcam (CA, United States). The anti-Opa-1 (sc-30573), anti-Mfn-1 (sc-166644), anti-Drp-1 (sc-101270), anti-Fis-1 (sc-376447), and anti-β-actin (sc-47778) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States).
5
Enzymatic Activity Profiling in Aquatic Organisms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, the enzymatic activities of pepsin, lipase, and α-amylase in the intestine and stomach were detected using a Pepsin Assay Kit, a Lipase Assay Kit, and an α-Amylase Assay Kit (Nanjing Jiancheng, Bioengineering Institute, China). The enzymatic activities of ACP, AKP, LDH, SOD, CAT, GPT, and GOT in the gill, brain, intestine, stomach, kidney, liver, and plasma were detected using an Acid Phosphatase Assay Kit, an Alkaline Phosphatase Assay Kit, a Lactate Dehydrogenase Assay Kit, a Superoxide Dismutase Assay Kit, a Catalase (CAT) Assay Kit, an Alanine Aminotransferase Assay Kit, and an Aspartate Aminotransferase Assay Kit (Nanjing Jiancheng Bioengineering Institute, China). Total protein was determined with a Total Protein Assay Kit (Nanjing Jiancheng Bioengineering Institute, China). The operation steps were carried out according to the operation guide of the reagent kit, and the operation guide of the corresponding reagent kit can be searched for on the website (http://www.njjcbio.com/ , accessed on 8 June 2023). In addition, the calculation formulas for these enzyme activities are detailed in the Supplemental Material.
6
Membrane Permeability Assessment via LDH
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The lactate dehydrogenase (LDH) content can be used to determine the permeability of cell membranes. As the method used to measure the zeta potential, the cell pellets were exposed to aqueous ozone and were subsequently disrupted ultrasonically. The LDH content was then measured using an LDH activity assay kit (Nanjing Jiancheng Bioengineering Institute, China). Cells that were neither treated with aqueous ozone nor ultrasonically processed were used as the spontaneous group. Cells that were lysed directly by ultrasound were set as the maximum LDH released group. The damage ratio of the cell membrane was expressed by the following equation: A total superoxide dismutase (T-SOD) assay kit (Nanjing Jiancheng Bioengineering Institute, China) and a catalase (CAT) assay kit (Nanjing Jiancheng Bioengineering Institute, China) were used to measure the intracellular SOD and CAT activities, respectively. The measuring methods and equations were the same as those used for LDH in accordance with the manual.
7
Physiological Stability in Poplar Leaves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Without the influence of external factors, the physiological indexes of the mature leaves (functional leaves) of poplar are relatively stable [71 (link)]. The 5th–7th healthy mature leaves of each plant from the four treatment groups were sampled and quickly placed in the ice box. Then, the physiological and biochemical indexes were measured in the laboratory immediately. A total of 0.2 g of each leaf sample was weighted and ground with 1.5 mL of the phosphate buffer. The homogenized liquid was transferred to a 5 mL tube for the determination of malonaldehyde (MDA), hydrogen peroxide (H2O2), Superoxide Dismutase (SOD), Catalase (CAT), and Ascorbate peroxidase (APX). The content of MDA was determined using the Thiobarbituric acid method, and the absorption values at 600 nm, 532 nm, and 450 nm wavelengths were recorded [72 (link)]. The contents of H2O2, SOD, CAT, and APX were determined with the Hydrogen Peroxide assay kit, Total Superoxide Dismutase (T-SOD) assay kit (Hydroxylamine method), Catalase (CAT) assay kit (Visible light), and Ascorbate peroxidase (APX) test kit (Nanjing Jian cheng Biological Engineering Institute, Nanjing, China), respectively. There were 9 biological replicates and 3 technical replicates for each treatment.
8
Assessing rice leaf responses to C. medinalis infestations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The O2− production rate, content of H2O2, MDA, and activities of SOD, POD, and CAT activities were measured to analyze the biochemical responses of various rice leaves to 4th instar larvae of C. medinalis. The O2− production rate and content of H2O2 were measured using Superoxide Anion Content Assay Kit and Hydrogen Peroxide (H2O2) Content Assay Kit (Beijing Boxbio Science & Technology Co., Ltd., Beijing, China), respectively. The determination of MDA content and activities of SOD, POD, and CAT were carried out using Plant Malondialdehyde (MDA) assay kit (Colorimetric method), Superoxide Dismutase (SOD) assay kit (WST-1 method), Peroxidase assay kit and Catalase (CAT) assay kit (Visible light) (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively. Using the above method, 0.05 g of rice leaves from different infested and uninfested varieties by the 4th instar C. medinalis were collected. Rice leaves, and 200 μL extract were ground in an ice bath as enzyme sources. The homogenate was centrifuged at 3,500 rpm for 10 min at 4 °C to separate the supernatant. Three biological replicates were set for each group. According to the kit instructions, the absorbance was determined using a microplate reader.
9
Antioxidant and Oxidative Stress Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The plant samples were firstly ground into powder in liquid nitrogen and then suspended in ice-cold phosphate buffer (0.1 M, pH = 7). The sample was vortexed at maximum speed for 1 min and centrifuged at 12,000 rpm for 15 min. The supernatants were then used for further determination of the hydrogen peroxide level and antioxidant enzyme activity. The hydrogen peroxide level of different samples was determined using the Hydrogen Peroxide Assay Kit (Jiancheng Bioengineering Institute, Nanjing, China). The absorbance at 405 nm was determined by a Scandrop spectrophotometer (Analytikjena, Germany). The hydrogen peroxide level was calculated based on a previously described formula [56 (link)]. The MDA, proline, and total soluble sugar level were separately determined using the MDA Assay Kit, the Proline Assay Kit, and the Plant Soluble Sugar Content Test Kit (Jiancheng Bioengineering Institute, Nanjing, China), as previously described [24 (link)].
The antioxidant enzyme activities were also determined using the spectrophotometric method. SOD, POD, and CAT activities were separately determined using the Total Superoxide Dismutase (T-SOD) Assay Kit, Peroxidase Assay Kit, and Catalase (CAT) Assay Kit (Jiancheng Bioengineering Institute, Nanjing, China), as previously described [56 (link)].
The antioxidant enzyme activities were also determined using the spectrophotometric method. SOD, POD, and CAT activities were separately determined using the Total Superoxide Dismutase (T-SOD) Assay Kit, Peroxidase Assay Kit, and Catalase (CAT) Assay Kit (Jiancheng Bioengineering Institute, Nanjing, China), as previously described [56 (link)].
10
Antioxidant Enzyme Activity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After spleen removal, 10% spleen tissue homogenates were prepared in an ice-water bath, and centrifuged at 1000 rpm for 10 min at 4°C. Supernatants were then stored at -80°C until enzyme activity analysis. The protein concentration of the supernatants was measured with the Coomassie brilliant blue G250 method. The activities of antioxidant enzyme were evaluated using Na + /K + -Adenosine Triphosphatase (ATPase) assay kit, Total Superoxide Dismutase (T-SOD) assay kit, CATalase (CAT) assay kit and Glutathione Peroxidase (GSH-Px) assay kit (Nanjing Jiancheng Bioengineering Institute, China) according to commercial kit specifications.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!