Advia 2400 chemistry system analyzer

Manufactured by Siemens

Sourced in United States

The ADVIA 2400 Chemistry System is an automated clinical chemistry analyzer that performs various laboratory tests, including routine chemistry, immunoassay, and specialty testing. It is designed to handle a high volume of samples with speed and precision. The ADVIA 2400 is capable of processing a wide range of clinical chemistry tests to support diagnostic and monitoring needs in healthcare settings.

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Lab products found in correlation

Hydragel 7 protein kit, by Sebia (1 mentions) Pageruler prestained protein ladder, by Thermo Fisher Scientific (1 mentions) Coomassie plus protein assay kit, by Thermo Fisher Scientific (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Glucometer, by LifeScan (1 mentions) A110 1 1, by Nanjing Jiancheng (1 mentions) Perfusion medium, by Thermo Fisher Scientific (1 mentions) Oleic acid, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Liver digest medium, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

ADVIA 2400 (272 citations) ADVIA 2400 Chemistry System (109 citations) ADVIA 2400 analyzer (15 citations) ADVIA Chemistry (15 citations) ADVIA Chemistry System (13 citations) Advia 2400 Chemistry Analyzer (4 citations) Advia 2400 system (3 citations) Advia 2400 chemistry (2 citations)

16 protocols using advia 2400 chemistry system analyzer

Liver Function Evaluation in Animals

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Liver function was evaluated in the animals by determining serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations using an ADVIA 2400 Chemistry System analyzer (Siemens, Tarrytown, NY) according to the manufacturer’s instructions.

Huang Z., Wu L., Zhang J., Sabri A., Wang S., Qin G., Guo C., Wen H., Du B., Zhang D., Kong L., Tian X., Yao R., Li Y., Liang C., Li P., Wang Z., Guo J., Li L., Dong J, & Zhang Y. (2019). Dual Specificity Phosphatase 12 Regulates Hepatic Lipid Metabolism Through Inhibition of the Lipogenesis and Apoptosis Signal–Regulating Kinase 1 Pathways. Hepatology (Baltimore, Md.), 70(4), 1099-1118.

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Evaluating Liver Function Markers

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Liver tissue TG, total cholesterol (TC), and nonesterified fatty acid (NEFA) levels were measured using commercial kits (Wako, Osaka, Japan), while liver functions were evaluated in the animals by determining the serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase using an ADVIA 2400 Chemistry System analyzer (Siemens, Tarrytown, NY), according to the manufacturer’s instructions.

Ye P., Liu J., Xu W., Liu D., Ding X., Le S., Zhang H., Chen S., Chen M, & Xia J. (2019). Dual‐Specificity Phosphatase 26 Protects Against Nonalcoholic Fatty Liver Disease in Mice Through Transforming Growth Factor Beta–Activated Kinase 1 Suppression. Hepatology (Baltimore, Md.), 69(5), 1946-1964.

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Comprehensive Protein Analysis Protocol

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The total protein concentration was determined by the Bradford method (Coomassie Plus Protein Assay Kit, Thermo, Waltham, MA, USA), and IgG, IgA, and IgM concentrations by sandwich enzyme-linked immunosorbent assay (ELISA) kits (Human IgG, IgA, and IgM ELISA Quantitation Set, Bethyl Laboratories, Montgomery, TX, USA) [20 (link)]. Complements C3 and C4 were measured with a polyethylene glycol (PEG)-enhanced immunoturbidimetric method using an ADVIA 2400 Chemistry System analyzer (Siemens Healthcare Diagnostics, city?, NY, USA). Albumin determination used a bromocresol green solution (BCG) as a binding dye, and the albumin-BCG complex was measured as an endpoint reaction at 596/694 nm. The purity of the gamma protein was assessed by zone electrophoresis using a Hydragel 7 protein kit (Sebia, Evry, France) [22 (link)]. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing or reducing conditions was run using a NuPAGE 4%~12% Bis-Tris Gel (Invitrogen, Carlsbad, CA, USA) and a PageRuler prestained protein ladder (Thermo). Separated proteins were stained with Protein Gel Fast Stain (Strong Biotech, Taipei, Taiwan) [22 (link)].

Weng Y.J., Husebekk A., Skogen B., Kjaer M., Lin L.T, & Burnouf T. (2016). Anti-Human Platelet Antigen-1a Immunoglobulin G Preparation Intended to Prevent Fetal and Neonatal Alloimmune Thrombocytopenia. PLoS ONE, 11(9), e0162973.

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Liver Function Assessment Protocol

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Liver function was evaluated by determining the serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) using an ADVIA 2400 Chemistry System analyzer (Siemens, Tarrytown, NY), according to the manufacturer’s instructions.

Ye P., Xiang M., Liao H., Liu J., Luo H., Wang Y., Huang L., Chen M, & Xia J. (2018). Dual‐Specificity Phosphatase 9 Protects Against Nonalcoholic Fatty Liver Disease in Mice Through ASK1 Suppression. Hepatology (Baltimore, Md.), 69(1), 76-93.

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Serum Cytokine and Metabolic Profiling

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Cytokine concentrations in mouse serum were examined via ELISA (#SEA133Mu for TNF and #SEA087Mu for MCP1, Cloud-Clone Corp., Wuhan, China). Serum TG, TC, ALT, AST, ALP, LDL-c, HDL-c, LDH, CK, CKMB, UREA and CRE levels were measured by using an ADVIA 2400 Chemistry System analyzer (Siemens, Tarrytown, NY, USA) according to the manufacturer’s instructions.

Jian C., Fu J., Cheng X., Shen L.J., Ji Y.X., Wang X., Pan S., Tian H., Tian S., Liao R., Song K., Wang H.P., Zhang X., Wang Y., Huang Z., She Z.G., Zhang X.J., Zhu L, & Li H. (2020). Low-Dose Sorafenib Acts as a Mitochondrial Uncoupler and Ameliorates Nonalcoholic Steatohepatitis. Cell metabolism, 31(5), 892-908.e11.

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Metabolic Markers in Mice Study

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The fasting blood glucose levels and body weight levels of the mice were assessed every 4 weeks, and the fasting blood glucose levels were measured using a glucometer (Life Scan, Milpitas, CA, USA). Serum TC, ALT, and AST concentrations were measured using an ADVIA 2400 Chemistry System Analyzer (Siemens, Tarrytown, NY, USA) according to the manufacturer’s instructions.

Jiang L., Zhao J., Yang Q., Li M., Liu H., Xiao X., Tian S., Hu S., Liu Z., Yang P., Chen M., Ye P, & Xia J. (2023). Lysosomal-associated protein transmembrane 5 ameliorates non-alcoholic steatohepatitis by promoting the degradation of CDC42 in mice. Nature Communications, 14, 2654.

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Measuring Hepatic and Serum Lipid Profiles

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Commercial kits were used to measure the contents of triglyceride (TG) in primary hepatocytes (290-63701; Wako; Tokyo, Japan), according to the manufacturer’s instructions. The serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), TG, TC, and LDL-C were measured by an ADVIA 2400 Chemistry System Analyzer (Siemens, Tarrytown, NY, USA), according to the manufacturer’s instructions.

Lin Z., Yang P., Hu Y., Xu H., Duan J., He F., Dou K, & Wang L. (2023). RING finger protein 13 protects against nonalcoholic steatohepatitis by targeting STING-relayed signaling pathways. Nature Communications, 14, 6635.

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Metabolic Characterization of Mice

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The weight and blood glucose levels of the mice were measured after fasting for 6 h every 4 weeks. Blood insulin levels were estimated using an enzyme-linked immunosorbent assay kit (Cloud-Corp, Wuhan, China). Homeostatic model assessment of insulin resistance was evaluated using the following formula: (fasting blood glucose [mmol/l] × fasting serum insulin [mIU/l])/22.5. Glucose tolerance test assays were performed at the 23rd week as described previously: animals were injected (ip) at a dose of 1 g/kg after 6 h of fasting. Blood glucose levels were measured at baseline and at 15, 30, 60, and 120 min after glucose injection, and the area under the curve was calculated using the conventional trapezoid rule.
Levels of serum lipid triglyceride, total cholesterol, alanine aminotransferase, and aspartate aminotransferase were measured using an ADVIA 2400 Chemistry System Analyzer (Siemens, Tarrytown, NY).

Ding T., Chen S., Xiao W., Liu Z., Tu J., Yu Y., Dong B., Chen W, & Zeng Y. (2022). Six-Transmembrane Epithelial Antigen of Prostate 3 Promotes Hepatic Insulin Resistance and Steatosis. Journal of Lipid Research, 64(1), 100318.

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Hepatic Lipid Triglyceride Quantification

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The hepatic lipid triglyceride (TG) contents were quantified by using commercial TG kits (A110-1-1, Nanjing Jiancheng Bioengineering Institute) according to the manufacturer’s directions. To assess the lipid content in the plasma and evaluate liver function, the serum levels of TGs, total cholesterol (TC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were evaluated in the animals. For the analysis, an ADVIA 2400 Chemistry System analyzer (Siemens, Tarrytown, NY) was employed, following the guidelines provided by the manufacturer.

Yang Z., Zhang S., Liu X., Shu R., Shi W., Qu W., Liu D., Cai Z., Wang Y., Cheng X., Liu Y., Zhang X.J., Bai L., Li H, & She Z.G. (2024). Histone demethylase KDM1A promotes hepatic steatosis and inflammation by increasing chromatin accessibility in NAFLD. Journal of Lipid Research, 65(3), 100513.

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Measuring Liver Metabolites and Function

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The TG, TC, and NEFA contents in the livers of mice were detected using commercial kits as described above. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the serum were examined using an ADVIA 2400 Chemistry System analyzer (Siemens, Tarrytown, NY) to evaluate liver function.

Lu Y., Jiang Z., Dai H., Miao R., Shu J., Gu H., Liu X., Huang Z., Yang G., Chen A.F., Yuan H., Li Y, & Cai J. (2018). Hepatic leukocyte immunoglobulin‐like receptor B4 (LILRB4) attenuates nonalcoholic fatty liver disease via SHP1‐TRAF6 pathway. Hepatology (Baltimore, Md.), 67(4), 1303-1319.

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