ATG16L1 mouse mAb (MBL, M150‐3, 1:1,000), ATG5 rabbit pAb (CST, 2630, 1:1,000), ATP6V0A2 rabbit pAb (Abcam, ab96803), GABARAP (E1J4E) rabbit mAb (CST, 13733, 1:1,000), γ‐Tubulin (clone GTU‐88) mouse mAb (Sigma‐Aldrich, T6557, 1:10,000), GFP mouse mix of two monoclonal Ab (Roche, 11‐814‐460‐001), LC3B (D11) XP rabbit mAb (CST, 3868, 1:1,000), mCherry goat pAb (Origene AB0040‐200, 1:1,000), nsmase2 mouse pAb (Abcam, ab68735, 1:1,000), PI4K2A mouse mAb (Santa Cruz, sc‐390026), TECPR1 (D6C10) rabbit mAb (CST, 8097, 1:1,000), Vinculin (clone hVIN‐1) mouse mAb (Sigma‐Aldrich, V9131, 1:3,000), HRP goat anti‐mouse (Jackson ImmunoResearch, 115‐035‐003, 1:5,000), HRP donkey anti‐goat (Jacskon Immunoresearch, 115‐035‐004, 1:5,000) and HRP goat anti‐rabbit (Jackson ImmunoResearch, 111‐035‐144, 1:5,000).
Goat anti mouse hrp
Manufactured by Jackson ImmunoResearch
Sourced in United States, United Kingdom, Panama
Goat anti-mouse HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and visualize primary mouse antibodies in various immunoassays and immunochemical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit hrp, by Jackson ImmunoResearch (55 mentions)
Goat anti rat hrp, by Jackson ImmunoResearch (6 mentions)
Donkey anti rabbit hrp, by Jackson ImmunoResearch (5 mentions)
Anti β actin, by Merck Group (4 mentions)
Alexa fluor 680 goat anti mouse, by Thermo Fisher Scientific (4 mentions)
Goat anti rabbit hrp, by Thermo Fisher Scientific (4 mentions)
Chemidoc, by Bio-Rad (4 mentions)
Nitrocellulose membrane, by Bio-Rad (4 mentions)
Bca protein assay, by Thermo Fisher Scientific (4 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (4 mentions)
Imagequant las 4000, by GE Healthcare (3 mentions)
Pefabloc, by Roche (3 mentions)
Anti actin hrp, by Merck Group (3 mentions)
Mouse anti rabbit hrp, by Santa Cruz Biotechnology (3 mentions)
Mouse anti β catenin, by BD (3 mentions)
Ripk3, by Cell Signaling Technology (3 mentions)
Actin aan01 a, by Cytoskeleton (3 mentions)
Image lab 6, by Bio-Rad (3 mentions)
Protease inhibitor cocktail, by Merck Group (3 mentions)
Mini protean tgx gel, by Bio-Rad (3 mentions)
99 protocols using goat anti mouse hrp
1
Autophagy Protein Marker Detection
2
Western Blot Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors. Protein samples were separated by 8% SDS–PAGE, transferred to nitrocellulose membranes, blocked with 5% BSA, and incubated sequentially with primary and horseradish peroxidase (HRP)‐conjugated secondary antibodies, with washes. The primary antibodies used were anti‐MT1‐MMP (LEM‐2/15) (Galvez et al, 2001 ), anti‐eNOS (BD Bioscience, 610297), anti‐GFP (Abcam, ab13970), anti‐GAPDH (Sigma‐Aldrich, G9545), and anti‐β‐actin (Sigma‐Aldrich, A5441). All primary antibodies were used at a dilution of 1:1,000. The secondary antibodies used were HRP goat anti‐mouse and HRP goat anti‐rabbit (Jackson), and bound secondary antibodies were visualized with Luminata Classico Western HRP Substrate (Millipore, WBLUC0500) in ImageQuant LAS 4000 (GE Healthcare Life Sciences, Massachusetts, USA). Western blots were quantified using ImageJ software (https://imagej.nih.gov/ij/ ).
3
Antibody detection protocol for neuroreceptors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following primary antibodies were used: rabbit anti-CB1R, mouse anti-β-actin, goat anti-β-Arrestin2 (β-A2), mouse anti-GluN2B and mouse anti-GLUA2 (Santa Cruz Biotechnology, Dallas, TX, USA); rabbit anti-GluN2A, rabbit anti-GluN1and rabbit anti-GluA1 (Alomone Labs, Jerusalem, Israel); rabbit anti-CB1R phospho S316 (pCB1R) (Abcam, Cambridge, UK), rabbit anti-ERK 1/2 phospho Thr202/Tyr204 (pERK 1/2) (Cell Signaling Technology, Danvers, MA, USA) and mouse anti-VGlut2 (Merck-Millipore, Burlington, MA, USA). Secondary Abs for immunoblotting include HRP-donkey anti-rabbit and HRP-goat anti-mouse (Jackson ImmunoResearch, West Grove, PA, USA). For immunofluorescence, the secondary Abs used were donkey anti-goat Alexa Fluor 488, donkey anti-mouse Alexa Fluor 555, and donkey anti-rabbit Alexa Fluor 647, all from Invitrogen (Thermo Fisher Scientific, San Diego, CA, USA).
4
Protein Quantification and Immunoblotting Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein concentrations were quantitated using the DC Protein Assay (Bio‐Rad, Hercules, CA). Equal concentrations of protein were resolved by SDS‐Page using 4% to 12% NuPAGE Novex 4% to 12% Bis‐Tris Protein Gels and MES SDS Running Buffer (Thermo Fisher). Proteins were either stained with Coomassie blue to visualize total protein or transferred to nitrocellulose membranes (Millipore Corp., Billerica, MA) for immunoblot analysis. For immunoblot analysis, membranes were blocked with 5% blotto for 1 hour at room temperature and incubated with primary antibodies at 4°C, on a shaker, overnight. Primary antibodies included: Cell Signaling31 : (p38: #9212 Pp38: #4511 ERK: 9102 pERK: 9101), Developmental Hybridoma Bank32 : (anti‐myc tag: 9E10, anti‐MyHC: F59), (anti‐α Myosin heavy chain: BA‐G5), (anti‐α‐Sarcomeric Actin: mouse IgM isotype, clone 5C5, Sigma #A2172), (anti‐β Myosin Heavy Chain mouse monoclonal IgG1, Vector Laboratories #VP‐M667). Secondary Antibodies for cell signaling blots: HRP‐goat anti rabbit, Jackson cat# 115‐035‐114 for Developmental hybridoma bank blots: HRP‐goat anti mouse, Jackson cat# 115‐035‐003, #115‐035‐020 for sarcomeric actin. Blots were developed using Western Lightning Plus Chemiluminescence Substrate (Perkin Elmer) and an ImageQuant LAS 4000 (GE).
5
Histone Purification and Antibody Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein was prepared from frozen thymi using T-PER (Thermo Scientific) with protease inhibitors (Roche). Histones were purified from whole cell lysates using the Histone Purification Mini Kit (Active Motif). Antibodies were used at manufacturer-recommended concentrations from Active Motif: H3K4me1 (39297), H3K4me3 (39915), H3K9me2 (39753), H3K27me3 (39155), H3 (61277), Cell Signaling Technology: IKAROS (5443), Cleaved NOTCH1 (Val1744) (4147), Sigma-Aldrich: FLAG M2 (F1804), Bio-Rad: HRP-goat-anti-mouse, and Jackson Immunoresearch: HRP-goat-anti-rabbit.
6
Optimized Antibody Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HRP donkey anti‐rabbit (1:5,000) and HRP goat anti‐mouse (1:5,000) were purchased from Jackson ImmunoResearch (West Grove, PA).
7
Secondary Antibody Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
HRP donkey anti-rabbit (1:5000) and HRP goat anti-mouse (1:3000) from Jackson ImmunoResearch (West Grove, PA, USA) were used as secondary antibodies.
+ Expand
HRP donkey anti-rabbit (1:5000) and HRP goat anti-mouse (1:3000) from Jackson ImmunoResearch (West Grove, PA, USA) were used as secondary antibodies.
8
Western Blot Analysis of E. coli RNA σ54
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Overnight bacteria cultures were treated with Cell Lytic B Lysis Reagent (Sigma) to generate crude cell lysates. The soluble protein fraction was separated on 10% Mini-PROTEAN TGX Stain-Free protein gels (BioRad), activated for 5 minutes with UV light, imaged and transferred via semi-dry apparatus to a PVDF membrane. Membranes were incubated with primary antibody specific for E. coli RNA σ54 (1:500, BioLegend) overnight, then with secondary antibody HRP goat anti-mouse (1:10,000, Jackson ImmunoResearch). The chemiluminescent signal was generated with the Pierce SuperSignal West Fempto substrate kit (Thermo Scientific) and detected with ChemiDoc MP Imaging System (Bio-Rad Laboratories). Protein bands and total protein per lane were measured with Image Lab (Version 5.2.1; Bio-Rad Laboratories). RpoN bands were compared to corresponding total detected protein in each lane and the background value in ∆rpoN was subtracted from all samples.
9
Immunofluorescence and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glyoxal solution (Cat# 50649) was from Sigma-Aldrich. Rabbit anti-VE-Cadherin (#2158) was from Cell Signaling and was diluted 1:1000 for Western blot analyses. Anti-VE-Cadherin antibody was diluted 1:100 for immunofluorescence assay. Horseradish peroxidase (HRP) conjugated goat antirabbit (sc-2054) secondary Ab was from Santa Cruz Biotechnology, while HRP-goat anti-mouse (115-035-003) was from Jackson ImmunoResearch Laboratories. Alexa Fluor 488 donkey anti-rabbit (A21206), Alexa Fluor 555 Phalloidin (A34055) and DAPI (D3571) were from Thermo Fisher Scientific. GAPDH (G-9) monoclonal HRP has been used in western blot 1:5000 Santa Cruz Biotechnology. RAGE Antagonist, FPS-ZM1 -Calbiochem was from Sigma-Aldrich.
10
Quantification of Huntingtin and Synuclein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Quantification of Htt and Synuclein, the latter being the main protein involved in pathology of PD, were determined by western blot analysis on pellets of resting or activated (thrombin or collagen) platelets from HD and from positive control patients with PD, respectively. Fifteen µg of platelet proteins were mixed with 1x DTT and 5x Sample buffer and then water was added to give a final volume of 30µl. The samples migrated for 105 minutes at 100V on a Tris-acetate gel (3-8%) and were transferred to a polyvinylidene difluoride (PVDF) membrane overnight at 20V followed by 20 minutes at 100V. Membranes were incubated with the primary mouse antihuntingtin antibody (1:1000, Milipore: Mab2166) or the mouse anti-Syn (1:16,5, AbCam: Ab75305) followed by an HRP-goat anti-mouse (1:250 000, Jackson ImmunoResearch laboratories: 115.035.166) to detect the protein of interest. Chemiluminescence was quantified using a ThermoScientific MyELC Imager. Immunoblot band intensity was quantified with ImageJ Analysis Software (National Institutes of Health, http://imagej.nih.gov/ij).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!