Ethylamine

Manufactured by Merck Group

Sourced in United States, Germany

Ethylamine is a chemical compound with the formula CH3CH2NH2. It is a colorless, flammable gas with a strong ammonia-like odor. Ethylamine is used as a chemical building block in the synthesis of various pharmaceutical and industrial products.

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Lab products found in correlation

Close spelling variants of this product

2,2′-(ethylenedioxy)bis(ethylamine) (8 citations) Ethylamine hydrochloride (6 citations) 2-(3,4-dihydroxyphenyl) ethylamine (dopamine) hydrochloride (3 citations) 2-(3,4-Dihydroxyphenyl)ethylamine hydrochloride (dopamine hydrochloride), (2 citations) N-ethylamine (2 citations) 2-(4-chlorophenyl)ethylamine (2 citations) Ethylamine solution (2 citations)

20 protocols using ethylamine

HPLC Enantioseparation of Carvedilol

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Carvedilol, HPLC-grade solvents (n-hexane, 2-propanol (IPA), ethanol, ethyl acetate (EA), dichloromethane (DCM), acetonitrile (ACN) and amines (di ethylamine (DEA), tri ethylamine, buthylamine, exylamine, ethanolamine, ethylenediamine and ethylamine) were purchased from Sigma-Aldrich (Milan, Italy). HPLC enantioseparations were performed by using stainless-steel Chiralpak^® IG-3 (250 mm × 4.6 mm, 3 μm), Chiralpak^® IG (250 mm × 10 mm, 5 μm), Chiralpak^® IA-3 (250 mm × 4.6 mm, 3 μm) and Chiralpak^® IA (250 mm × 10 mm, 5 μm) columns (Chiral Technologies Europe, Illkirch, France).

Panella C., Ferretti R., Casulli A, & Cirilli R. (2019). Temperature and eluent composition effects on enantiomer separation of carvedilol by high-performance liquid chromatography on immobilized amylose-based chiral stationary phases. Journal of Pharmaceutical Analysis, 9(5), 324-331.

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Synthesis of Compounds 8 and 10

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3,3’-Dichloropivalic acid, triphenylphosphine, ethylamine, 4 M HCl/dioxane and dithiothreitol were purchased from Sigma-Aldrich, Europe (Taufkirchen, Germany). Cystamine dihydrochloride was purchased from Alfa-Aesar (Karlsruhe, Germany), β-Alanine from Fluka (Taufkirchen, Germany) and amoxicillin sodium salt from GlaxoSmithKline (Madrid, Spain). Solvents were obtained from Sigma-Aldrich and Merck (Madrid, Spain), and they were used as received without further purification unless stated. Compounds 8 and 10 were prepared according to a previously published synthetic procedure [20 (link),21 (link)].

Mesa-Antunez P., Collado D., Vida Y., Najera F., Fernandez T., Torres M.J, & Perez-Inestrosa E. (2016). Fluorescent BAPAD Dendrimeric Antigens Are Efficiently Internalized by Human Dendritic Cells. Polymers, 8(4), 111.

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Odorant Preparation and Concentrations

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Odorants were ammonium hydroxide (Fisher or VWR International), trim ethylamine (Sigma), dim ethylamine (Sigma), ethylamine (Sigma), butylamine (Sigma), cadaverine (Sigma), spermidine (ACROS), isoamylamine (ACROS), and benzaldehyde (Sigma). All odorants were diluted to generate 10% stocks, and serial dilutions were used to generate lower concentrations. All odorants were diluted in water, except benzaldehyde, which was diluted in paraffin oil (ACROS). Ammonia was used at concentrations from 0.0001% to 1% for electrophysiology and transcuticular imaging. Other odorants used for electrophysiological experiments were at 1% concentration. All odorants were used at a 0.01% concentration for antennal lobe GCaMP imaging. For behavioral assays in Figure 3L, ammonia was used at 0.3% and benzaldehyde at 1%. For behavioral assays in Figures S2G and S2H, ammonia was used from 0.001% to 1% concentrations.

Vulpe A., Kim H.S., Ballou S., Wu S.T., Grabe V., Gonzales C.N., Liang T., Sachse S., Jeanne J.M., Su C.Y, & Menuz K. (2021). An ammonium transporter is a non-canonical olfactory receptor for ammonia. Current biology : CB, 31(15), 3382-3390.e7.

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Synthesis and Characterization of Functionalized Graphene

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Silver acetate [Ag(OCOCH₃), 99%], ethylamine (C₂H₅NH₂, 2.0
M in methanol), ammonium hydroxide (NH₄OH, 28.0–30.0%
NH₃ basis), formic acid (HCOOH, 98.0%), graphite (Sigma-Aldrich,
Asbury Carbon, 3061), potassium permanganate (KMnO₄, 99.0%),
H₂O₂ (30 wt % in water), potassium phosphate
monobasic (KH₂PO₄, 99.0%), MgCl₂,
KCl, CaCl₂, Na₂SO₄, thiamine pyrophosphate
chloride (TPP), 0.1 M N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES), ethanol, ethanolamine (99.0%),
and hydroxyethyl cellulose (HEC, 2% in water–methanol solution)
were purchased from Sigma-Aldrich. All aqueous solutions were prepared
using Milli-Q water purified deionized water.

Bhat K.S., Nakate U.T., Yoo J.Y., Wang Y., Mahmoudi T, & Hahn Y.B. (2019). Nozzle-Jet-Printed Silver/Graphene Composite-Based Field-Effect Transistor Sensor for Phosphate Ion Detection. ACS Omega, 4(5), 8373-8380.

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Kinetic Analysis of VAP-1 Enzyme Activity

Cited 1 time

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Putative, naturally occurring substrates of human VAP-1 were selected based on inclusion in the human metabolome database V.2.5 (http://www.hmdb.ca), and with reference to Shen et al,20 (link) specifically cysteamine, methylamine, dopamine, ethylamine and phenethylamine (Sigma). A 96-well plate was loaded with 50 ng recombinant VAP-1 (Biolegend) in 100 µL Dulbecco's phosphate-buffered saline with increasing concentrations of test substrate supplemented to test wells. A H₂O₂ standard curve was created, and enzyme activity quantified using the Amplex-UltraRed detection system across increasing concentrations of test substrate for a fixed concentration of VAP-1 under atmospheric conditions. Kinetic parameters were estimated by Michaelis-Menten methodology (see online supplementary figure 1). Benzylamine served as an internal positive control.

Trivedi P.J., Tickle J., Vesterhus M.N., Eddowes P.J., Bruns T., Vainio J., Parker R., Smith D., Liaskou E., Thorbjørnsen L.W., Hirschfield G.M., Auvinen K., Hubscher S.G., Salmi M., Adams D.H, & Weston C.J. (2017). Vascular adhesion protein-1 is elevated in primary sclerosing cholangitis, is predictive of clinical outcome and facilitates recruitment of gut-tropic lymphocytes to liver in a substrate-dependent manner. Gut, 67(6), 1135-1145.

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Modulation of IL-2 Production in Activated T Cells

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In total, 1 × 10⁵ clone 1C116 or 2C21 cells, were cultured with each material (concentration ranges are described below) or vehicle control (dimethyl sulfoxide: DMSO) and 20 ng/mL PMA in 96-well U-bottom tissue culture plates in 200 μL of CCM for 24 h. The materials were 0–100 μg/mL flavonoids (quercetin, isoquercitrin, hyperoside, galangin, kaempferol, morin, myricetin, hesperetin, hesperidin, acacetin, linarin, isorhoifolin, (all purchased from Extrasynthese, Lyon, France)), 0–1000 μM IPP (Sigma-Aldrich), 0–2000 μM risedronate, 0–33.5 μM m ethylamine (Sigma-Aldrich), 0–33.5 μM ethylamine (Sigma-Aldrich) and 1 μg/mL purified anti-human CD3 mAb (OKT3: Thermo Fisher Scientific). After the incubation, the culture supernatant was corrected, and the concentration of IL-2 was analyzed using an ELISA kit (BD Biosciense).

Yonekawa M., Shimizu M., Kaneko A., Matsumura J, & Takahashi H. (2019). Suppression of R5-type of HIV-1 in CD4+ NKT cells by Vδ1+ T cells activated by flavonoid glycosides, hesperidin and linarin. Scientific Reports, 9, 7506.

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Peptide Synthesis and Characterization

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Methanol, ammonium hydroxide and glacial acetic acid were purchased from Mallinckrodt (Phillipsburg, NJ). The peptides ARAAARA, AKAAARA, AKAAAKA, AHARAHARA, ARAMAKA, HGAGGHGAGGHL, AAEAAEAA and AADAADAA were custom synthesized by NeoBioLab (Cambridge, MA). The peptides ARACAKA, ARAWAKA were synthesized by Pepnome Ltd. (Shenzhen, China). The peptides GRGDSPR, LKRApYLG, LKRApYGL-NH₂ were custom synthesized by AnaSpec Inc. (San Jose, CA). The peptide GLSDGEWQQVLNVWGK was purchased from SynPep (Dublin, CA). Woodward’s reagent K, Angiotensin II (sequence: DRVYIHPF), ubiquitin from bovine erythrocytes, α-casein from bovine milk, TPCK-treated trypsin from bovine pancreas, peptide RPKPQQFFG, acetyl chloride and ethylamine were obtained from Sigma-Aldrich (St. Louis, MO). The reagent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) was purchased from Thermo Fisher Scientific (Rockford, IL). All materials were used without further purification. All peptide solutions for positive electrospray were prepared in 49.5/49.5/1 (v/v/v) Methanol/water/acetic acid (~10 μM), and all peptide solutions for negative electrospray were prepared in 49.5/49.5/1 (v/v/v) Methanol/water/ammonium hydroxide (~10 μM). The wrk reagent was dissolved in water (~2 mM), and a new solution was made before each experiment.

Peng Z., Pilo A.L., Luongo C.A, & McLuckey S.A. (2015). Gas-Phase Amidation of Carboxylic Acids with Woodward’s Reagent K Ions. Journal of the American Society for Mass Spectrometry, 26(10), 1686-1694.

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Ammonium Sulfate Protein Precipitation

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Ammonium sulfate (AS, ≥99.0%), glycine (Gly, ≥99%), ethylamine (EA, 66.0–72.0 wt% solution), propylamine (PA, ≥99%), hydroxyacetone (HA, 90%), glyoxal (GX, 40 wt% solution), methylglyoxal (MG, 40 wt% solution), and glycolaldehyde (GAld) were purchased from Sigma-Aldrich (USA). Acrolein (AC) was purchased from Aladdin (China). All chemicals were used as received without further purification. Ultrapure water (18.2 MΩ cm) was supplied by a Milli-Q water purification system.

Tang S., Li F., Lv J., Liu L., Wu G., Wang Y., Yu W., Wang Y, & Jiang G. (2022). Unexpected molecular diversity of brown carbon formed by Maillard-like reactions in aqueous aerosols. Chemical Science, 13(28), 8401-8411.

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Quantitative Amino Acid Analysis

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AccQ-Tag Ultra derivatization kit, a mixture of 17 proteinogenic amino acids (histidine, serine, arginine, glycine, aspartate, glutamate, alanine, threonine, proline, cysteine, lysine, tyrosine, methionine, valine, isoleucine, leucine, and phenylalanine) and AccQ-Tag Ultra eluent A and B were purchased from Waters (Milford, MA, USA). LC–MS grade water, 3kDa Nanosep columns and asparagine, glutamine, taurine, histamine, ethanolamine, m ethylamine, citrulline, ethylamine, ornithine, putrescine, serotonin, cadaverine, tyramine, tryptamine and 2-phenethyl-amine standards were obtained from Sigma-Aldrich (St. Louis, MI, USA). Stable isotope-labelled (SIL) tryptophan was purchased from Cambridge Isotope Laboratories (Tewksbury, MA, USA).

Guba A., Bába O., Tőzsér J., Csősz É, & Kalló G. (2022). Fast and Sensitive Quantification of AccQ-Tag Derivatized Amino Acids and Biogenic Amines by UHPLC-UV Analysis from Complex Biological Samples. Metabolites, 12(3), 272.

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Synthesis of Functionalized Chitosan Nanoparticles

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Chitosan (Ch) (degree of deacetylation: 75–85 %, Mwt: 190,000–310,000), hexylamine (HA), purity 99%, ethylamine (EA), 66.0–72.0 % in water, 1,2-diaminopropane (DAP), purity 99% N,N-dimethyldodecylamine (DDA), purity 97%, potassium carbonate, K₂CO₃, purity over 99%, sodium carbonate, Na₂CO₃, purity over 99.5%, potassium hydroxide, KOH, purity > 85%, urea (U), purity over 99.5%, aniline (A), purity over 99.5%, chromium(VI) oxide, CrO₃, purity 99.9%, and lead(II) nitrate, Pb(NO₃)₂, purity more than 99.0% were all purchased from Sigma-Aldrich (Schnelldorf, Germany). Dimethyldidodicylammonium bromide, DDAB, was prepared in our laboratory as described [42 (link)]. CaCO₃ nanoparticle (NPs) was purchased from Sky spring nanomaterials, (Houston, TX, USA) purity 99% and HCl (36.5–36 vol %) was purchased from J.T.Baker. All reagents and solvents were of analytical grade and were used without further purification. The N₂ gas used was 99.999% pure (KOAC, Kuwait). All solutions were prepared with deionized water of a resistivity of 18.2 MΩ.cm.

Emamy F.H., Bumajdad A, & Lukaszewicz J.P. (2021). Adsorption of Hexavalent Chromium and Divalent Lead Ions on the Nitrogen-Enriched Chitosan-Based Activated Carbon. Nanomaterials, 11(8), 1907.

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