Tecnai f20

Manufactured by Thermo Fisher Scientific

Sourced in United States, Netherlands, Japan, Germany

The Tecnai F20 is a high-performance transmission electron microscope (TEM) designed for advanced materials research and nanoscale imaging. It features a field emission electron source, providing high brightness and coherence for enhanced resolution and contrast. The Tecnai F20 enables detailed examination of a wide range of samples at the atomic scale.

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Tecnai G2 F20 S-TWIN (198 citations) Tecnai F20 microscope (89 citations) Tecnai F20 S/TEM (12 citations) Tecnai G2 F20 S-Twin microscope (10 citations) Tecnai™ G2 F20 TWIN Cryo-TEM (6 citations) Tecnai G2 F20 system (4 citations) Tecnai G2 F20 ST (3 citations) Tecnai G2 F20 X-TWIN Transmission Electron Microscope (TEM)) (2 citations) FEI Tecnai G2 F20 microscope (2 citations) Tecnai G2 F20 S-TWIN field emission TEM (2 citations)

449 protocols using tecnai f20

Characterization of Nanostructured Materials

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TEM images were taken on a conventional TEM (JEOL1400-Plus). High-resolution imaging was conducted on a HRTEM (FEI Tecnai F20) operated at 200 kV. The objective lens astigmatism of the HRTEM (FEI Tecnai F20) was corrected by using a standard carbon grating replica with Au/Pd particles. The live FFT pattern obtained from the amorphous carbon film was seen by tuning the ring. A nearly perfect round ring was obtained, indicating the reduction of astigmatism. We then saved the objective lens parameters (deflection currents). Subsequently, the SIO TEM samples were loaded and characterized. The element mapping of the SIO was performed on this HRTEM in scanning TEM (STEM) mode. During the optimization of reaction conditions, Z-contrast images and EDX spectra were taken on a STEM (Hitachi HD-2000) equipped with EDS detector (Bruker Quantax) at an accelerating voltage of 200 kV.
DLS measurements were obtained using a Malvern ZS 90 (Malvern Instruments). The absorbance was measured over time on a UV–vis spectrometer (SpectraMax from Molecular Devices) for colloidal stability evaluation. The nitrogen adsorption–desorption analysis was done at 77 K on a Micromimetics ASAP 2020 system. Magnetic hysteresis loops were measured by sweeping the field from −7 to 7 T at 300 K on a Quantum Design MPMS3 superconducting quantum interface device (SQUID) magnetometer.

Chen F., Zhao E.R., Hableel G., Hu T., Kim T., Li J., Gonzalez-Pech N.I., Cheng D.J., Lemaster J.E., Xie Y., Grassian V.H., Sen G.L, & Jokerst J.V. (2019). Increasing the Efficacy of Stem Cell Therapy via Triple-Function Inorganic Nanoparticles. ACS nano, 13(6), 6605-6617.

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Characterization of s-ONWST Electrical and Morphological Properties

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The electrical characteristics of s-ONWST were measured using a semiconductor parameter analyzer (Keithley 4200 and Keysight B1500) under N₂ in a glove box, and the response for the International Morse code was measured under ambient conditions. The morphology of ONW was measured using an optical microscope (Leica DM4000M), a scanning electron microscope (FEI XL30 Sirion), and a transmission electron microscope (FEI Tecnai F20 at 200 kV). The chemical composition of ONW was determined using an energy-dispersive x-ray spectroscope (FEI Tecnai F20 at 200 kV).

Lee Y., Oh J.Y., Xu W., Kim O., Kim T.R., Kang J., Kim Y., Son D., Tok J.B., Park M.J., Bao Z, & Lee T.W. (2018). Stretchable organic optoelectronic sensorimotor synapse. Science Advances, 4(11), eaat7387.

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Cryo-EM Imaging of Lipoprotein Interactions

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LUVs (100 μL) were incubated
with the respective lipoprotein solutions (5 μL) for 2 min at
room temperature. Immediately after incubation, samples were stored
on ice and applied to the cryo-grids (2 nm precoated Quantifoil R3/3
holey carbon-supported grids) at a concentration of 10 μM and
vitrified using a Vitrobot Mark IV (FEI). Data were collected on a
TEM microscope FEI Tecnai F20 equipped with a 4K CCD camera and two
side-entry cryo-holders. The data set was collected using a Tecnai
F20 instrument (FEI, Eindhoven, The Netherlands) operated at 200 kV
and equipped with a 4K charge-coupled device detector FEI Eagle. Micrographs
were collected with a pixel size of 1.79 Å and a total dose of
20 e^–/Å². Frames were aligned using
MotionCor2,^{23 (link)} and CTF parameters were estimated
using Gctf.^{24 (link)}

Plochberger B., Sych T., Weber F., Novacek J., Axmann M., Stangl H, & Sezgin E. (2020). Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors. Biochemistry, 59(45), 4421-4428.

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Comprehensive Characterization of Nanomaterials

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The surface morphology was observed by Zeiss EVO MA10 scanning electron microscope (SEM) and FEI Tecnai F20 field-emission transmission electron microscope (TEM). The elemental mapping was collected by the FEI Tecnai F20 equipped with an energydispersive X-ray spectroscope (EDS) under STEM mode. X-ray diffraction (XRD) patterns were measured with PANalytical X'Pert3 X-ray diffractometer. X-ray photoelectron spectroscopy (XPS) was performed by the Thermo Scientific K-Alpha + XPS spectrometer. All binding energies were calibrated with respect to C 1 s peak at 284.8 eV. Electron paramagnetic resonance (EPR) spectra were measured with a Bruker A300 spectrometer. Raman spectra were collected by a HORIBA iHR320 Raman microscope with a 514-nm laser. UV-visible absorption spectra were measured using Shimadzu UV-3600 spectrophotometer with an integrated sphere. Photoluminescence (PL) spectra were analyzed using Shimadzu RF-5301PC spectrometer. Time-resolved photoluminescence (TRPL) spectra were measured by the PicoQuant Fluo Time 200 spectrometer coupled with a TimeHarp 260 time-correlated single-photon counting (TCSPC) system.

Peng Y., Du M., Zou X., Jia G., Permatasari Santoso S., Peng X., Niu W., Yuan M, & Hsu H.Y. (2022). Suppressing photoinduced charge recombination at the BiVO₄||NiOOH junction by sandwiching an oxygen vacancy layer for efficient photoelectrochemical water oxidation. Journal of colloid and interface science, 608(Pt 2).

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Quantifying Myelin Integrity in Corpus Callosum

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Mice were perfused with 3% paraformaldehyde and 1% glutaraldehyde for resin embedding. A block of approximately 1x1x2mm³ was removed from the body of the corpus callosum at the level of the anterior-dorsal hippocampus. Semi thin sections were stained with 1% toluidine blue (Sigma-Aldrich), and examined by light microscopy. Detailed evaluation of myelin integrity in the corpus callosum was performed on uranyl acetate and lead citrate stained thin sections using a FEI Tecnai^TM F20 transmission electron microscope. Extent of myelination was quantitatively compared by determining g-ratios, which were calculated by dividing the diameter of the axon by the diameter of the entire myelinated fiber as previously described (Cheli et al., 2016 (link)). Furthermore, the percentage of myelinated axons from the total number of axons was calculated. At least five animals per experimental group and 300 fibers per animal were analyzed using the MetaMorph software (Molecular Devices).

Cheli V.T., Santiago González D.A., Zamora N.N., Lama T.N., Spreuer V., Rasmusson R.L., Bett G.C., Panagiotakos G, & Paez P.M. (2018). Enhanced oligodendrocyte maturation and myelination in a mouse model of Timothy syndrome. Glia, 66(11), 2324-2339.

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Mitochondrial Purity Analysis by TEM

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To determine the purity of the mitochondria isolated using the Percoll gradient method, the isolated mitochondrial pellets were washed twice by centrifugation with an isolation buffer at 6,900 × g for 10 min at 4°C. After the supernatant was discarded, the intact mitochondrial pellets were fixed with 2.5% glutaraldehyde in PBS and incubated overnight at 4°C. The mitochondrial specimen was dehydrated in a graded ethanol series of 60%, 70%, 80%, 90%, and 100% for 30 min each. The dehydrated specimen was substituted with propylene oxide and infiltrated with an Epon mixture. Ultrathin sections (60 nm) were prepared using an ultramicrotome (Leica, Germany) and placed on a copper grid for electrostaining. The prepared specimens were viewed and interpreted using the Tecnai^TM F20 (FEI, USA).

Choi J.M., Piao Y., Ahn K.H., Kim S.K., Won J.H., Lee J.H., Jang J.M., Shin I.C., Fu Z., Jung S.Y., Jeong E.M, & Kim D.K. (2023). Purification and Characterization of Mitochondrial Mg2+-Independent Sphingomyelinase from Rat Brain. Molecules and Cells, 46(9), 545-557.

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Characterization of MoSe2 Phototransistor

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Optical images of the hexagonal MoSe₂ TFT were taken using an optical microscope (BX51M, Olympus Co., JAPAN) with white light (100 W halogen lamp, U-LH100-3) in bright field imaging mode and a 50× objective lens. The TEM images and diffraction patterns were obtained using a transmission electron microscope (FEI Tecnai^TM F20) operated at an acceleration voltage of 200 kV. For TEM sample preparation, the sample was cut to a 3 mm disk and the backside of the sample was hand-polished and dimpled down to about 5–10 μm at the center of the sample. Then, the sample was ion-milled from the backsides at a 4.5° angle and at 4.5 kV using a Gatan PIPS^TM until the small hole at the center of the sample was made. The topography of the MoSe₂ phototransistor was measured using an AFM (XE7 Atomic Force Microscope, Park Systems, South Korea) under non-contact mode with a 0.2 Hz scan rate. The electrical characteristics of the phototransistor were measured using a parameter analyzer (Keithley 4200 SCS) at room temperature. The photoresponsive properties of the MoSe₂ phtotransistor were evaluated using an illumination system composed of a Nikon Ti-e microscope with an Acton SP2300 spectroscope and a Zolix TLS3900x-500 tunable light source.

Jung C., Kim S.M., Moon H., Han G., Kwon J., Hong Y.K., Omkaram I., Yoon Y., Kim S, & Park J. (2015). Highly Crystalline CVD-grown Multilayer MoSe2 Thin Film Transistor for Fast Photodetector. Scientific Reports, 5, 15313.

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Ultrastructural Analysis of Mouse Brain and Sciatic Nerve

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Mouse brains were perfused transcardially with 3% paraformaldehyde and 1% glutaraldehyde. The body of the corpus callosum at the anterior-dorsal level of the hippocampus was dissected and resin embedded. Sciatic nerves were fixed with 2% glutaraldehyde and then embedded in resin. Thin sections were stained with uranyl acetate and lead citrate and photographed with a FEI Tecnai^TM F20 transmission electron microscope as previously described [26 (link),27 (link)]. The g-ratio and the percentage of myelinated axons were determined semi-automatically and blind to the genotype of the sample in 20 randomly selected fields per sample using MetaMorph software (Molecular Devices). For all experimental conditions, data represent pooled results from at least 4 mice.

Santiago González D.A., Cheli V.T., Rosenblum S.L., Denaroso G, & Paez P.M. (2021). Ceruloplasmin deletion in myelinating glial cells induces myelin disruption and oxidative stress in the central and peripheral nervous systems. Redox Biology, 46, 102118.

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Electron Tomography Imaging Workflow

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Electron tomography (ET) was performed as previously described^{48 (link)} Tomographic fiducial markers (15 nm protein A-coupled gold beads) were adsorbed to both sides of the EM grids. Transmission electron microscopy (TEM) images for correlation, and Scanning transmission electron microscopy (STEM) tilt series were acquired using a Tecnai F20 (Thermo Fischer Scientific) at 200 kV, using SerialEM^{49 (link)}. The TEM images for correlation were acquired at 4.47 nm pixel size using an UltraScan 4000 camera (Gatan). Tilt series were acquired over a 60° to −60° tilt range (1° increment) at 1.67 nm pixel size in nanoprobe mode using the bright-field detector, with a 10-µm C2 aperture and 320 mm camera length. The tomograms were reconstructed using IMOD software package (versions 4.9.4)^{50 (link)}. Correlations of fluorescence and TEM images were performed using the ec-CLEM plugin with the Icy software^{51 (link)}.

Elbaz-Alon Y., Guo Y., Segev N., Harel M., Quinnell D.E., Geiger T., Avinoam O., Li D, & Nunnari J. (2020). PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria. Nature Communications, 11, 3645.

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Characterization of Synthesized Materials

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The crystal structures of all synthesized materials were characterized by X-ray diffraction (XRD) (MiniFlex600; Rigaku, Tokyo, Japan) using a monochromatic Cu-Kα radiation source (λ = 1.541862Å). XRD patterns were recorded from 20 to 80° with a step size of 0.02° at the scan rate of 10 °/min. Transmission electron microscopy (TEM) (JEM-200FX, JEOL, Tokyo, Japan), high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) (Tecnai F20, Thermo Fisher Scientific, Waltham, MA, USA), and field emission scanning electron microscopy (FE-SEM) (JSM 7600 F, JEOL, Tokyo, Japan) were used for morphological analysis of the samples. The loading amounts of Pt on ZnO were determined by X-ray fluorescence (XRF) analysis (Epsilon 1, Malvern Panalytical, Malvern, UK).

Shinkai T., Masumoto K., Iwai M., Inomata Y, & Kida T. (2022). Study on Sensing Mechanism of Volatile Organic Compounds Using Pt-Loaded ZnO Nanocrystals. Sensors (Basel, Switzerland), 22(16), 6277.

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