Plates were coated overnight at + 4 °C with 5 μg/ml sheep anti-mouse IgA (Sigma-Aldrich) or goat anti-mouse IgG (Southern Biotech, France) in PBS. Plates were blocked with PBS-5% fetal calf serum (FCS) (Invitrogen) before incubation with diluted samples or purified IgA or IgG (Southern Biotech). Horseradish-peroxidase (HRP)-conjugated goat anti-mouse IgA (Sigma-Aldrich) or goat anti-mouse IgG (Southern Biotech) were added, HRP was revealed using TMB (Becton Dickinson, France). Reaction was stopped adding H2SO4 2 N and plates were analysed using automatic Infinite M200 microplate reader. Measurements were performed in duplicate and detection threshold correspond to at least three blank values.
Goat anti mouse igg
Goat anti-mouse IgG is a purified polyclonal antibody produced in goats and specifically binds to mouse immunoglobulin G (IgG). It is commonly used as a secondary antibody in immunoassays and other applications that require the detection of mouse primary antibodies.
Lab products found in correlation
71 protocols using goat anti mouse igg
Quantifying Immunoglobulins in Plasma and Feces
Plates were coated overnight at + 4 °C with 5 μg/ml sheep anti-mouse IgA (Sigma-Aldrich) or goat anti-mouse IgG (Southern Biotech, France) in PBS. Plates were blocked with PBS-5% fetal calf serum (FCS) (Invitrogen) before incubation with diluted samples or purified IgA or IgG (Southern Biotech). Horseradish-peroxidase (HRP)-conjugated goat anti-mouse IgA (Sigma-Aldrich) or goat anti-mouse IgG (Southern Biotech) were added, HRP was revealed using TMB (Becton Dickinson, France). Reaction was stopped adding H2SO4 2 N and plates were analysed using automatic Infinite M200 microplate reader. Measurements were performed in duplicate and detection threshold correspond to at least three blank values.
Mouse Immunoglobulin ELISA Protocol
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Comprehensive Immunological Assay Protocol
Autoantibody Profiling in Mouse Models
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Measuring Intestinal sIgA Levels by ELISA
Quantification of Mouse Ig Isotypes
SARS-CoV-2 S Protein ELISA for Antibodies
Western Blot Analysis of AKT1
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