Inveon micropet ct scanner

CT images were taken from rats 1 month after implantation of the closed-loop optogenetic control (CLOC) system with a Siemens Inveon Micro PET/CT Scanner.

All animal experiments were performed in compliance with the Guidelines for the Care and Use of Research Animals as established by the Stanford University Animal Studies Committee. Small animal PET/CT scans were performed on A431 tumor-bearing mice (n = 4) using a Siemens Inveon microPET/CT scanner. Each nude mouse was injected with 11.1 MBq (300 μCi) of ⁶⁴Cu-PEG-MNPs via tail vein. Inhaled anesthesia was performed on each nude mouse with 2% isoflurane–oxygen gas mixture. Thirty minute static scans were performed at 2, 4, 8 and 24 h after the injection. All images were reconstructed by a two-dimensional ordered subsets expectation maximum (OSEM) algorithm with attenuation and scatter correction.
For biodistribution study, A431 tumor-bearing mice were divided into 4 groups and each mouse was injected with 11.1 MBq (300 μCi) of ⁶⁴Cu-PEG-MNPs. After 2, 4, 8 and 24 h of injection, the nude mice were sacrificed and dissected. Blood, tumor and other major organs (heart, lung, spleen, liver, stomach, kidney, intestine, bone and muscle tissue) were collected and weighed; the radioactivity was measured by the γ counter. The percentage of the injected radioactive dose per gram of tissue (% ID/g) value was calculated and expressed as mean ± standard deviation.

All experiments were performed in accordance with the Stanford University Animal Care and Use Committee Guidelines and approved APLAC protocols. For evaluation of in vivo osteogenesis, nonhealing, critical-size (4 mm) calvarial defects were created in the right parietal bone of adult (60-day-old) female CD-1 nude mice, as previously described.^{21 (link)} Implants, as described above, containing MC encoding for BMP-2, luciferase, or GFP were placed into the defect space (n=3 per group). For micro-CT scans, the mice were anesthetized with 2–3% inhaled isoflurane. Imaging was performed using a Siemens Inveon MicroPET/CT scanner (Siemens Medical Solutions Inc., Malvern, PA). Using our scan protocol parameters, each high resolution 100 μm image was acquired in a total scan time of 10 min. Mice were immediately scanned postoperatively and then at 2-, 4-, 6-, 8-, and 12-weeks following surgery. Data were reconstructed into 3D surfaces using Inveon Research Workplace 4.0 (Siemens Medical Solutions Inc.). The 3D reconstructed images were then analyzed using ImageJ by quantifying pixels in the defect. Percentage healing was determined by dividing the area of regenerated bone by the baseline defect size.