EGCg, ECg, GCg, Cg, EGC, EC, and GC were purchased from Kurita Water Industries Ltd. (Tokyo, Japan). (+)-Catechin, Na2HPO4·12H2O NaH2PO4·2H2O, NaCl, and ibuprofen were purchased from Wako Pure Chemicals Co. (Tokyo, Japan). Ethyl gallate (EtGa) was purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). (−)-Epigallocatechin-3′-O-methylether gallate (EGCg-3′-O-Me), (−)-epigallocatechin-4′-O-methylether gallate (EGCg-4′-O-Me), (−)-epigallocatechin-3′′-O-methylether gallate (EGCg-3′′-O-Me), and (−)-epigallocatechin-4′′-O-methylether gallate (EGCg-4′′-O-Me) were purchased from Nagara Science Co. Ltd. (Gifu, Japan). Warfarin was purchased from Nacalai Tesque (Kyoto, Japan). HSA was purchased from Sigma-Aldrich Japan (Tokyo, Japan). We used a non-degreased HSA. The HSA was dissolved in a phosphate buffer and the HSA stock solution was prepared by extensive overnight dialysis at 4°C. The purity of HSA was checked by using SDS-PAGE.
Ibuprofen
Manufactured by Fujifilm
Sourced in Japan
Ibuprofen is a pharmaceutical product used for the analysis and quantification of ibuprofen, a common non-steroidal anti-inflammatory drug (NSAID). It is a laboratory equipment designed to accurately measure the concentration of ibuprofen in various samples, such as pharmaceutical formulations, biological fluids, or environmental samples. The core function of this product is to provide researchers and analysts with a reliable and precise tool for the detection and quantification of ibuprofen, which is essential for quality control, pharmacokinetic studies, and environmental monitoring.
Automatically generated - may contain errors
Lab products found in correlation
Human serum albumin (hsa), by Merck Group (2 mentions)
Allopurinol, by Fujifilm (2 mentions)
Azathioprine, by Fujifilm (2 mentions)
Isoniazid, by Fujifilm (2 mentions)
Metronidazole, by Fujifilm (2 mentions)
Phenytoin, by Fujifilm (2 mentions)
Valproic acid, by Fujifilm (2 mentions)
Ketoprofen, by Fujifilm (2 mentions)
Carbamazepine, by Fujifilm (2 mentions)
Chlorpromazine, by Fujifilm (2 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Catechin, by Fujifilm (1 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
Ethyl gallate, by Tokyo Chemical Industry (1 mentions)
Dicloxacillin, by Merck Group (1 mentions)
Diclofenac, by Fujifilm (1 mentions)
Diphenhydramine, by Fujifilm (1 mentions)
Flutamide, by Fujifilm (1 mentions)
Imipramine, by Fujifilm (1 mentions)
Indomethacin, by Fujifilm (1 mentions)
10 protocols using ibuprofen
1
Interaction of Catechin Derivatives with Serum Albumin
2
Comprehensive Pharmacological Compound Database
3
Probing PEPT1-Mediated Glycylsarcosine Uptake
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The culture medium was removed and the cells were preincubated with the transport buffer (Hanks’ balanced salt solution containing 10 mM 2-(N-morpholino)ethanesulfonic acid (MES), pH 6.0) at 37 °C for 15 min. The uptake assays were initiated by replacing the medium with transport buffer containing 135 nM [3H]glycylsarcosine at 37 °C in the presence or absence of 3 mM ibuprofen (Wako Pure Chemical Industries) (PEPT1 inhibitor) or at 4 °C. Uptake was stopped by the addition of ice-cold transport buffer; the cells were rinsed using the same buffer. The cells were lysed with 0.2 M NaOH solution (0.5 mL/well) containing 0.5% sodium dodecyl sulfate (SDS). Radioactivity was measured through liquid scintillation counting, using 3 mL Clear-sol I (Nacalai Tesque, Inc., Kyoto, Japan) as the scintillation fluid. The total protein in differentiated cells was measured using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific).
4
Purification and Analysis of ALDH2
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Tunicamycin, 4-phenylbutylate, cycloheximide, ibuprofen, and lysozyme were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The ALDH2 protein (18–517 aa) was obtained from ATGen (Seoul, Korea). (±)-Flurbiprofen were from Sigma (St Louis, MO, USA) or Cayman Chemical (Ann Arbor, MI, USA). α-Lactalbumin, aspirin, and meloxicam were from Sigma. Human recombinant leptin for use in vitro was from Sigma and mouse recombinant leptin for use in vivo was from R'D Systems (Minneapolis, MN, USA). Ferriteglycidyl methacrylate (FG) beads (epoxy beads: TAS8848N1110) were from Tamagawa Seiki (Tokyo, Japan). 4′-hydroxy flurbiprofen was obtained from Toronto Research Chemicals (Toronto, ON, Canada).
5
Adsorption and Heavy Metal Removal Using Biopolymers
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DNA sodium salt (>5 kbp, ca. 90% purity), CS (50 kDa–190 kDa, 75–85% deacetylation), and D-(+)-glucono-δ-lactone (GDL) were purchased from Sigma-Aldrich (St. Louis, MI, USA). Sodium hydroxide (NaOH), 6 M solution of hydrochloric acid, and sodium chloride (NaCl) were used as received. Congo Red from Kishida Chemical Co., Ltd. (Osaka, Japan) and 1% Methylene Blue solution (MB) form Kanto Chemical Co., Inc. (Tokyo, Japan) were used for dye adsorption experiments. Carbamazepine, ibuprofen, and ketoprofen were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan); clofibric acid, paracetamol, thymol, and 3,3’-Dihydroxydiphenylamine were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan); and phenazone was purchased from Sigma-Aldrich (Japan). Standard solutions of 100 ppm (JCSS) of Cd(NO3)2, Cu(NO3)2, HgCl2, and Pb(NO3)2 in 0.1 M HNO3 were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Milli-Q water was purified by Purelab Chorus 1 Life Science apparatus and used in all experiments.
6
Procurement of Diverse Chemical Compounds
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Abietic acid, ADD, DHEA, methyltestosterone,
phenanthrene, and progesterone were purchased from Tokyo Chemical
Industry (Tokyo, Japan). DehydroAbietic acid, dibenzothiophene, 7-ethoxycoumarin,
ibuprofen, and testosterone were purchased from Fujifilm Wako Pure
Chemical (Osaka, Japan). Pyrene was purchased from Sigma-Aldrich Japan
(Tokyo, Japan). Diclofenac was purchased from Combi-Blocks USA (San
Diego, CA, USA). Ferruginol was kindly provided by Dr. H. Suhara (Miyazaki
Prefectural Wood Utilization Research Center, Japan). Yeast nitrogen
base without amino acids was purchased from Formedium (Hunstanton,
UK). Dropout supplements (DOS) were purchased from TaKaRa Bio USA
(Mountain View, CA, USA). Custom-synthesized oligonucleotide primers
were obtained from Sigma-Aldrich Japan. All other chemicals were of
reagent grade. Deionized water was obtained using a Barnstead Smart2Pure
System (Thermo Fisher Scientific, Waltham, MA, USA).
phenanthrene, and progesterone were purchased from Tokyo Chemical
Industry (Tokyo, Japan). DehydroAbietic acid, dibenzothiophene, 7-ethoxycoumarin,
ibuprofen, and testosterone were purchased from Fujifilm Wako Pure
Chemical (Osaka, Japan). Pyrene was purchased from Sigma-Aldrich Japan
(Tokyo, Japan). Diclofenac was purchased from Combi-Blocks USA (San
Diego, CA, USA). Ferruginol was kindly provided by Dr. H. Suhara (Miyazaki
Prefectural Wood Utilization Research Center, Japan). Yeast nitrogen
base without amino acids was purchased from Formedium (Hunstanton,
UK). Dropout supplements (DOS) were purchased from TaKaRa Bio USA
(Mountain View, CA, USA). Custom-synthesized oligonucleotide primers
were obtained from Sigma-Aldrich Japan. All other chemicals were of
reagent grade. Deionized water was obtained using a Barnstead Smart2Pure
System (Thermo Fisher Scientific, Waltham, MA, USA).
7
Mucoadhesive Drug Delivery Formulations
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Methanol, polyvinylpyrrolidone (K30; PVP), ketoprofen (KTP), flurbiprofen (FBP), ibuprofen (IBP), loxoprofen (LXP), mucin (from porcine stomach), phosphate-buffered saline (PBS), and trifluoroacetic acid were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Alginic acid sodium salt (Alg, from brown algae with low viscosity) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydroxypropyl methyl cellulose HPMC (TC-5® M) was purchased from Shin-Etsu Chemical Co. Ltd. (Tokyo, Japan). All other chemicals used were of reagent grade.
8
Toxicity Assessment of Commonly Used Drugs
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The following drugs were used for toxicity assessment in this study: amiodarone (TCI, Tokyo, Japan, A2530-1G), atorvastatin (TCI, A2476-1G), clotrimazole (Wako, Tokyo, Japan, 035-16021), aspirin (Wako, 015-10262), cyclosporin A (Wako, 031-24931), chlorpheniramine (Wako, 030-13271), chlorpromazine (Wako, 033-10581), and ibuprofen (Wako, 098-02641). All drugs were diluted with DMSO to the desired concentrations.
9
Protein Binding Affinity Assay
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Materials Silibinin, HSA (fraction V, fatty acid free), α 1 -acid glycoprotein (AAG), γ-globulins, warfarin, iodipamide, dansyl-L-asparagine (DNSA) and 2-hydroxy-5-nitrobenzyl bromide (HNB) were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). Sodium chloride (NaCl) and polyoxyethylene lauryl ether (PLE, hydroxyl number; 40-60) were obtained from Nacalai Tesque, Inc. (Kyoto, Japan) and Cosmo Bio Co., Ltd. (Tokyo, Japan), respectively. Ibuprofen was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Human plasma was obtained from Interstate Blood Bank, Inc. (Memphis, TN, U.S.A.). All other chemicals from commercial sources were of the highest grade. Sixty-seven micromole sodium phosphate buffer (pH 7.4) was used in the protein binding experiments.
10
Pharmacological Compound Acquisition for Research
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Materials The following 47 compounds were purchased from Wako Pure Chemical Industries, Ltd., Osaka, Japan for use in this study: acetylsalicylic acid, acyclovir, allopurinol, amitriptyline, amoxicillin, azathioprine, azithromycin, budesonide, caffeine, carbamazepine, chloramphenicol, chlorpheniramine maleate salt, chlorpromazine, diethylcarbamazine, digoxin, diphenhydramine hydrochloride, doxycycline, erythromycin, ethosuximide, fluconazole, folic acid, furosemide, haloperidol, hydrochlorothiazide, hydrocortisone, ibuprofen, isoniazid, loratadine, metformin, metoclopramide, metronidazole, nitrofurantoin, ondansetron, oseltamivir, paracetamol, phenytoin, potassium iodide, prednisolone, primaquine, propranolol, propylthiouracil, quinine hydrochloride, salbutamol, spironolactone, sulfamethoxazole, valproic acid and vancomycin.
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