Chokeberry fruits were bought at the local market. Alginic acid sodium salt (very low viscosity) was obtained from Alfa Aesar (Kandel, Germany), while low-methoxyl pectin (Genu pectin type LM-5-CS) was from CP Kelco (Lille Skensved, Denmark). Calcium chloride, ethanol, sodium acetate, potassium chloride and ammonium acetate were from Gram-mol (Zagreb, Croatia). Sodium carbonate was purchased from T.T.T. (Sveta Nedelja, Croatia). Potassium persulfate and Folin-Ciocalteu reagent were procured from Kemika (Zagreb, Croatia). Cupric chloride, neocuproine and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were bought from Acros Organic (Geel, Belgium). methanol (HPLC grade) was from J.T. Baker (Deventer, The Netherlands) and orthophosphoric acid (HPLC grade, >85%) was from Fisher Scientific (Loughborough, UK). Hydrochloric acid (37%) and methanol were bought from Carlo Erba Reagents (Sabadell, Spain) and acetic acid (>99.5%) was from Alkaloid (Skopje, North Macedonia). Trolox, 2,2-diphenyl-1-picrylhydrazil, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 4-dimethyl-amino-cinnamaldehyd and most of the standards used for HPLC analysis (chlorogenic acid, quercetin and rutin) were from Sigma-Aldrich (St. Louis, MO, USA), while the standards of cyanidin-3-galactoside, neochlorogenic acid and hyperoside were products of Extrasynthese (Genay, France).
Folin ciocalteu reagent
Manufactured by Kemika
Sourced in Croatia
The Folin–Ciocalteu reagent is a colorimetric assay used for the quantitative determination of phenolic and certain other easily oxidized compounds. It is a mixture of phosphomolybdic and phosphotungstic acid complexes.
Automatically generated - may contain errors
Lab products found in correlation
Sodium carbonate, by Kemika (12 mentions)
Trolox, by Merck Group (10 mentions)
Gallic acid, by Merck Group (9 mentions)
Hydrochloric acid (hcl), by Kemika (7 mentions)
Chlorogenic acid, by Merck Group (6 mentions)
Methanol, by Kemika (6 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (6 mentions)
Quercetin, by Merck Group (5 mentions)
Caffeic acid, by Merck Group (5 mentions)
Epicatechin, by Merck Group (5 mentions)
Potassium persulfate, by Kemika (4 mentions)
Neocuproine, by Thermo Fisher Scientific (4 mentions)
2 2 diphenyl 1 picrylhydrazil, by Merck Group (4 mentions)
Rutin, by Merck Group (4 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (4 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (4 mentions)
Potassium chloride (kcl), by Kemika (4 mentions)
Cupric chloride, by Thermo Fisher Scientific (3 mentions)
Hydrochloric acid (hcl), by Carlo Erba (3 mentions)
Dichlorodihydrofluorescein diacetate, by Merck Group (3 mentions)
21 protocols using folin ciocalteu reagent
1
Chokeberry Fruit Phytochemical Analysis
2
Development of Stable Sunflower Oil Emulsions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sunflower oil (Solesta, Aldi, Fermoy, Ireland), purchased in a local supermarket, was used as oil phase for preparation of double emulsions (DEs). The lipophilic emulsifier used to stabilize the colloidal emulsions, polyglycerol polyricinoleate (PGPR), was supplied by Danisco (Copenhagen, Denmark), while whey protein isolates (WPI), used as hydrophilic emulsifiers in outer aqueous phase and consisted of 95% β-lactoglobulin, were from Davisco Foods International (Le Sueur, MN, USA). Maltodextrins with average dextrose equivalent values of 10 and 21 (MDE 10 and MDE 21) were obtained from Grain Processing Corporation (Muscatine, IA, USA). Deionised water was used for preparation of solutions. Folin-Ciocalteu reagent, sodium carbonate and formic acid were supplied by Kemika (Zagreb, Croatia), while ethanol was from Carlo-Erba (Peypin, France); all chemicals were of analytical grade. Gallic acid and HPLC standards, ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt], DPPH (2,2-diphenyl-1-picrylhydrazyl), Trolox (6-hydroxy--2,5,7,8-tetramethylchromane-2-carboxylic acid), and Nile blue were obtained from Sigma-Aldrich (Steinheim, Germany). Acetonitrile and methanol (HPLC grade) were purchased from J.T.Baker (Deventer, the Netherlands).
3
Antioxidant Assays and Zebrafish Analyses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fatty acids methyl esters (FAMEs) were purchased from Supelco Co. (Bellefonte, PA, USA). The standards of L-ascorbic acid (≥99%), gallic acid (>97.5%), DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tripyridyl-S-triazine, ≥98%), ABTS (diammonium salt of 2,2′-azino-bis(3-ethylbenzthiazolin-6-yl)sulfonic acid, >99.0%), and dichloro-dihydro-fluorescein diacetate (≥97%, DCF-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Organic solvents (dimethyl sulfoxide (DMSO, p.a.), ethanol (p.a.), methanol (p.a.)), hydrochloric acid (HCl, p.a.), iron (III) chloride (FeCl3, p.a.), Folin–Ciocalteu reagent and NaHCO3 (p.a.) were obtained from Kemika (Zagreb, Croatia), while potassium persulfate (>98%) was purchased from Scharlab (Barcelona, Spain). Hydrogen peroxide (H2O2, 30%) was obtained from Alkaloid Skopje (Skopje, North Macedonia).
Acetonitrile with 0.1% (v/v) formic acid and water with 0.1% (v/v) formic acid, both hypergrade for HPLC-MS LiChrosolv®, were purchased from Supelco Co. (Bellefonte, PA, USA).
Zebrafish D. rerio adults of wild-type WIK strain were obtained from the European Zebrafish Resource Center of the Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Used solvents were of HPLC grade and were obtained from J.T. Baker (Bridgewater, NJ, USA).
Organic solvents (dimethyl sulfoxide (DMSO, p.a.), ethanol (p.a.), methanol (p.a.)), hydrochloric acid (HCl, p.a.), iron (III) chloride (FeCl3, p.a.), Folin–Ciocalteu reagent and NaHCO3 (p.a.) were obtained from Kemika (Zagreb, Croatia), while potassium persulfate (>98%) was purchased from Scharlab (Barcelona, Spain). Hydrogen peroxide (H2O2, 30%) was obtained from Alkaloid Skopje (Skopje, North Macedonia).
Acetonitrile with 0.1% (v/v) formic acid and water with 0.1% (v/v) formic acid, both hypergrade for HPLC-MS LiChrosolv®, were purchased from Supelco Co. (Bellefonte, PA, USA).
Zebrafish D. rerio adults of wild-type WIK strain were obtained from the European Zebrafish Resource Center of the Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Used solvents were of HPLC grade and were obtained from J.T. Baker (Bridgewater, NJ, USA).
4
Comprehensive Analytical Standards for Lipid and Phytochemical Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All standards of fatty acid methyl esters were purchased from Sigma (St. Louis, MO, USA). Methanol, n-hexane, petroleum ether, chloroform, potassium iodide, Folin–Ciocalteu reagent, sodium carbonate, starch, concentrated sulphuric, nitric and hydrochloric acid (37%) were purchased from Kemika (Zagreb, Croatia). Anhydrous sodium sulphate and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) were purchased from Sigma (St. Louis, MO, USA).
Sodium and potassium hydroxides in the form of pellets, chloroform and analytical standards (dihydrocholesterol, campesterol, stigmasterol, β-sitosterol, eicosanol, docosanol, tricosanol, tetracosanol, pentacosanol, hexacosanol, heptacosanol, octacosanol, squalene and (±)-α-tocopherol) were purchased from Sigma (St. Louis, MO, USA). Ethanol and a silylating mixture according to Sweeley were purchased from Fluka (Buchs, Switzerland).
Hydroxytyrosol, tyrosol, oleuropein, lutein and apigenin were purchased from Extrasynthese (Genay, France). Caffeic acid and gallic acid were purchased from Sigma (St. Louis, MO, USA). Standard stock solutions of each compound were 1 mg/mL in Methanol.
All mineral standards were purchased from Merck (Darmstadt, Germany) and diluted to the appropriate obtained concentration.
All standards stored at +4 °C in amber vials and in a dark place until analysis.
Sodium and potassium hydroxides in the form of pellets, chloroform and analytical standards (dihydrocholesterol, campesterol, stigmasterol, β-sitosterol, eicosanol, docosanol, tricosanol, tetracosanol, pentacosanol, hexacosanol, heptacosanol, octacosanol, squalene and (±)-α-tocopherol) were purchased from Sigma (St. Louis, MO, USA). Ethanol and a silylating mixture according to Sweeley were purchased from Fluka (Buchs, Switzerland).
Hydroxytyrosol, tyrosol, oleuropein, lutein and apigenin were purchased from Extrasynthese (Genay, France). Caffeic acid and gallic acid were purchased from Sigma (St. Louis, MO, USA). Standard stock solutions of each compound were 1 mg/mL in Methanol.
All mineral standards were purchased from Merck (Darmstadt, Germany) and diluted to the appropriate obtained concentration.
All standards stored at +4 °C in amber vials and in a dark place until analysis.
5
Quantification of Total Phenols in Oils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total phenols were extracted from oil diluted with Hexane by liquid-liquid extraction with methanol/water (60:40, V/V) according to the method of Gutfinger (21 (link)). The content of total phenols was estimated based on the phenol reaction with the Folin-Ciocalteu reagent and sodium carbonate, and measurement of absorbance at 725 nm with spectrophotometer (Carry UV/Vis 50; Varian Inc., Harbor City, CA, USA). caffeic acid was used as reference for the calibration curve and expression of results (mg caffeic acid per kg of oil). Hexane of analytical quality, methanol of spectrophotometric grade and caffeic acid (≥98%) were obtained from Sigma-Aldrich, Merck (Steinheim, Germany), and Folin–Ciocalteu reagent and sodium carbonate of analytical quality were obtained from Kemika (Zagreb, Croatia). Grade 2 water was obtained from Elix 3 system (Millipore, Bedford, MA, USA).
6
Analysis of Dairy Protein Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dairy proteins were obtained from Ingredia Functional (Arras, France). Methanol used for analysis was HPLC grade and it was obtained from J.T. Baker (Deventer, The Netherlands). Orthophosphoric acid was also HPLC grade (>85%) and it was purchased from Fisher Scientific (Loughborough, UK). 4-dimethyl-amino-cinnamaldehyd, trolox, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazil and standards for HPLC analysis (rutin, quercetin, chlorogenic acid, p-coumaric acid, (−)-epicatechin) were products of Sigma-Aldrich (St. Louis, MO, USA). The standard of cyanidin-3-rutinoside was from Phytolab GmbH (Vestenbergsgreuth, Germany) and the standard of neochlorogenic acid was the product of Extrasynthese (Genay, France). Folin-Ciocalteu reagent and potassium persulfate were products of Kemika (Zagreb, Croatia). Neocuproine, cupric chloride and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were products of Acros Organic (Geel, Belgium). Sodium carbonate was bought from T.T.T. (Sveta Nedelja, Croatia) and hydrochloric acid (37%) from Carlo Erba Reagents (Sabadell, Spain).
7
Phytochemical Analysis of Botanical Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals and reagents used in the experiments were of per analysis and HPLC grade. Folin–Ciocalteu reagent, potassium chloride, sodium hydrogencarbonate, potassium hydrogenphosphate, magnesium chloride hexahydrate, sodium chloride, ammonium carbonate, sodium hydroxide, and petroleter were supplied from Kemika d.d. (Zagreb, Croatia). Anhydrous sodium carbonate and sodium nitrite were acquired from Gram-Mol d.o.o. (Zagreb, Croatia). Sodium molybdate (>98%) was supplied from abcr Gmbh (Karlsruhe, Germany). Methanol, acetonitrile, concentrated hydrochloric acid (37%, v/v), sodium hydroxide, and formic acid were purchased from Carlo Erba Reagents (Val de Reuil, France). Secondary analytical standards, i.e., echinacoside (>98%) and verbascoside (>98%), were obtained from Biosynth s.r.o. (Bratislava, Slovakia), whereas caffeine was supplied from Fluka (Taufkirschen, Germany). Theobromine (min. 99%), human salivary α–amylase XII-A (Enzyme Commission number: 3.2.1.1.; 1149 U mg−1 protein), pepsin from porcine gastric mucosa (474 U mg−1 protein), pancreatin from porcine pancreas (4×USP), and bile bovine salts (unfractionated) were provided from Sigma Aldrich (St. Louis, MO, USA).
8
Tart Cherry Jam Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tart cherries were purchased at a local market, pits were removed, and flesh was used for the preparation of jams. The following chemicals were used: Potassium chloride, sodium acetate, hydrochloric acid, methanol, sodium carbonate, sodium bisulfite, Folin-Ciocalteu reagent were purchased from Kemika (Zagreb, Croatia). Trolox, 2,2’-azinobis (3-ethylbenzothiazoline sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazil (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
9
Antioxidant Evaluation of Citrus Fibers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hydrochloric acid, acetic acid, methanol, sodium carbonate, iron chloride, ammonium acetate and Folin–Ciocalteu reagent were purchased from the manufacturer Kemika (Zagreb, Croatia). Trolox was purchased from Sigma (Darmstadt, Germany). 2,2’-azinobis (3-ethylbenzothiozolinesulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Fluka (Darmstadt, Germany). 2,4,6,-tri (2-pyridyl)-s-triazine (TPTZ), gallic acid, procyanidin B2 and myrtenol were purchased from Sigma, Germany. Neocuproine and copper chloride are products of Gram-mol Zagreb, Croatia). Sucrose was purchased from Gram-mol (Zagreb, Croatia), and maltose and trehalose were obtained from Hayashibara doo (Nagase group, Tokyo, Japan). Citrus fibers were obtained from Biesterfeld AG (Zagreb, Croatia).
10
Analytical Standards for Food Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Folin−Ciocalteu reagent was purchased from Kemika (Zagreb, Croatia); 2,2-diphenyl-1-picrylhydrazyl, catechin (CAS: 154–23–4, ≥99.0%), epicatechin (CAS: 490–46–0; ≥98%), gallic acid (CAS: 149–91–7, ≥97.5), p-cumaric acid (CAS: 501–98–4, ≥98.0%), phloretin (CAS: 60–82–2, ≥99.0%), phloridzin (CAS: 60–81–1, ≥98%), chlorogenic acid (CAS: 327–97–9, ≥95%) and caffeic acid (CAS: 331–39–5, ≥98.0%) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Methanol (HPLC grade) and orthophosphoric acid (85%) were obtained from Panareac (Barcelona, Spain). Certified standard of patulin and MycoSep® 228 AflaPat columns were obtained from Romer Labs Biopure (Romer Labs, Tulln, Austria). Certified pesticide standard solutions were obtained from CPAchem (CPAchem, Stara Zagora, Bulgaria). LC-MS and HPLC grade acetonitrile was purchased from J.T. Baker (J.T. Baker, Deventer, The Netherlands), while LC-MS grade formic acid and LC-MS ammonium formate were produced by Sigma–Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Ammonium hydroxide solution, ≥25% NH3 in H2O, was purchased from Honeywell (Offenbach, Germany). Nylon syringe filters, 0.2 µm pore size and 13 mm diameter, were obtained from Agilent (Santa Clara, CA, USA). Ultrapure water was generated by Niro VV system (Nirosta d.o.o., Osijek, Croatia).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!