UV-vis spectra were recorded at room temperature on an Agilent Technologies 8453 UV-Vis spectrophotometer using quartz cuvettes with a path length of 1 cm and Jasco v770 spectrophotometer equipped with an integrating sphere for reflectance measurements.
V 770 spectrophotometer
Manufactured by Jasco
Sourced in Japan, United States, Germany
The V-770 spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light in a sample across a range of wavelengths. It is capable of performing precise measurements in the ultraviolet, visible, and near-infrared regions of the electromagnetic spectrum.
Automatically generated - may contain errors
Lab products found in correlation
Ht7700, by Hitachi (2 mentions)
Zetasizer nano z, by Malvern Panalytical (2 mentions)
Zetasizer nano series, by Malvern Panalytical (2 mentions)
Nicolet is10 ft ir spectrometer, by Thermo Fisher Scientific (2 mentions)
Fp 8500 spectrofluorometer, by Jasco (2 mentions)
Calf thymus dna ct dna, by Merck Group (1 mentions)
Xe 100, by Park Systems (1 mentions)
D8 endeavor, by Bruker (1 mentions)
Xplora, by Horiba (1 mentions)
Trisodium citrate dehydrate, by Kanto Chemical (1 mentions)
Haucl4, by Merck Group (1 mentions)
3 aminopropyltriethoxysilane aptes, by Merck Group (1 mentions)
S 4300, by Hitachi (1 mentions)
Methyl alcohol, by Samchun (1 mentions)
D8 advance diffractometer, by Hitachi (1 mentions)
Jsm 7610f fe sem, by JEOL (1 mentions)
Ft ir 6600, by Jasco (1 mentions)
Nova nanosem 450, by Thermo Fisher Scientific (1 mentions)
Quantax 200, by Bruker (1 mentions)
D2 phaser, by Bruker (1 mentions)
51 protocols using v 770 spectrophotometer
1
UV-Vis Spectroscopy Protocol
2
DNA Interaction Study of Compounds
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All chemicals were reagent grade, purchased from commercial vendors. They were used as purchased. Calf thymus DNA (ct-DNA) was obtained from Sigma Aldrich, UV–Vis JASCO V-770 spectrophotometer was employed to check DNA purity (A260: A280 > 1.80) and concentration (€ = 6600 M−1 cm−1 at 260 nm) [16 ]. Interactions of the compounds with ct-DNA were studied using solutions of the compound in DMSO and ct-DNA in Tris–HCl buffer (pH 7.2) containing 5 mM Tris–HCl. The buffer solution was prepared with double-distilled water.
3
Tannin Effects on Erythrocyte Hemolysis
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The effect of S. aureus NCTC 5655 and 8325–4 on the integrity of sheep erythrocytes was detected basing on the Olchowik-Grabarek et al. method with some modifications22 (link). Shortly, sheep blood was centrifuged (2,655 × g, 15 min, 4 °C) and plasma and buffy coat were removed by aspiration. Erythrocytes were washed twice with 0.9% NaCl, and then 1% suspension was prepared in 0.9% NaCl. 2 ml of 1% suspension of erythrocytes was incubated 30 min at 37 °C in the presence or absence of tannins. Then 100 µl of bacteria in MH broth (A620 = 2.4) was added to each sample. After incubation for 60 min at 37 °C, 0.5 ml of suspension was taken from every sample and mixed with 1 ml of 0.9% NaCl. For obtaining of 100% hemolysis 1 ml of water was added to the control sample. All the samples were centrifuged, and absorbance of supernatants was measured using Jasco V-770 spectrophotometer (Japan) at 540 nm.
In the study of the αHL—induced hemolysis, 2 ml of 1% suspension of sheep erythrocytes in 0.9% NaCl was incubated with and without tannins for 30 min. Then the suspension was incubated for 45 min with αHL (50 nM final concentration). All the samples were centrifuged, and absorbance of supernatants was measured at 540 nm. Obtained results are presented as percent of hemolysis depending on the concentration of tannins.
In the study of the αHL—induced hemolysis, 2 ml of 1% suspension of sheep erythrocytes in 0.9% NaCl was incubated with and without tannins for 30 min. Then the suspension was incubated for 45 min with αHL (50 nM final concentration). All the samples were centrifuged, and absorbance of supernatants was measured at 540 nm. Obtained results are presented as percent of hemolysis depending on the concentration of tannins.
4
Organic Field-Effect Transistor Characterization
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Ultraviolet–visible (UV-vis) absorption spectra of both solutions and thin films were acquired by using a V-770 spectrophotometer (Jasco, Inc., Easton, MD, USA). The thickness and surface morphologies of the thin films were investigated using an atomic force microscope (AFM; XE-100, Park Systems, Suwon, Korea). The OFETs and sensor devices were placed in an environment-controlled air-tight chamber and characterized by using Keithley 2634B (Tektronix, Beaverton, OR, USA) and HP4156A (Keysight Technologies, Santa Rosa, CA, USA) semiconductor parameter analyzers. The field-effect mobility (μ) and threshold voltage (VT) of the devices were calculated by using the curves of saturation regime (Vd = −100 V) and equation: Id = (WCi/2L)µ(Vg − VT)2, where Id is the drain current, Ci is the capacitance per unit area of dielectric (17.3 nF/cm2), and Vg is the gate voltage. As an ammonia source, a 1-N ammonia solution was vaporized to obtain the target concentration (ppm) in the chamber. The carrier gas was nitrogen. The chemical response characteristics were quantified based on the current changes upon analyte exposure.
5
Spectroscopic Characterization of PEDOT:PSS/GO-AuNPs Thin Films
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The morphology of the samples was analyzed using SEM (Hitachi S4800) and HRTEM (Jeon-JEM 2100). A JASCO V-770 spectrophotometer was used to measure the absorption spectra. The XRD pattern was measured by using a XRD Bruker D8 Endeavor equipped with CuKα radiation. The Raman spectra of samples were recorded by using XploRA, Horiba Jobin-Yvon spectroscopy. The NPS Sigma-5+ based on 4-point technique were used to measure the sheet resistance of the PEDOT:PSS/GO-AuNPs thin film. The PCE of the hybrid solar cells was measured with a Keithley 2400 source meter under AM 1.5 G with 100 mW cm−2 illumination.
6
Gold Nanoparticle Synthesis and Characterization
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HAuCl4 (≥99.9%) and (3-aminopropyl)-triethoxysilane (APTES; ≥9 8.0%) were purchased from Sigma-Aldrich (USA). Trisodium citrate dehydrate was obtained from Kanto Chemical Co., Inc. (Japan) and methyl alcohol (99.5%) was acquired from Samchun Pure Chemical Co., Ltd. (Korea). Glass substrates, and all other chemicals were of analytical grade and used as received. Phosphate-buffered saline (PBS, pH 7.4) was prepared using 0.01 M Na2HPO4 and 0.01 M NaH2PO4. Ultraviolet/Visual (UV/Vis) spectrophotometry was conducted using a V-770 spectrophotometer (Jasco International Co., Ltd, Japan). Field emission scanning electron microscopy (SEM, S-4300, HITACHI, Japan) images were also taken.
7
Ag/ZnO Nanohybrid Characterization
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The X-ray crystallographic study was conducted with a Bruker D8 Advance diffractometer and the morphological analysis was by a Hitachi S-4800 microscopy operated at 15 kV. A JEOL 1010 at 100 kV was used to obtain the transmission electron micrograms. The elemental mapping of the Ag/ZnO nanohybrid was evaluated by a JEOL JSM-7610F FE-SEM. A Jasco V-770 spectrophotometer was utilized for analyzing the optical properties and the photodegradation study of the as-prepared samples. All the electrochemical studies were performed using Metrohm Autolab. A three-electrode system with Ag/AgCl as a reference electrode, a platinum wire as a counter electrode, and the prepared nanocomposite-modified SPCE as a working electrode was used for the electrochemical experiments.
8
Comprehensive Materials Characterization Protocol
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X-ray diffraction (XRD) was conducted on a D2 PHASER (Bruker, Germany) and patterns were obtained using CuKα radiation. Fourier transform infrared (FTIR) spectroscopy was conducted by using a Jasco FT/IR-6600. The Brunauer-Emmett-Teller (BET) surface area analysis was conducted on a TriStar II (Micromeritics, GA, USA) was obtained from the N2 adsorption/desorption isotherm using a fully automatic physisorption analyzer. Scanning electron microscopy (SEM) was conducted on a Nova NanoSEM 450 (FEI, OR, USA) at an acceleration voltage of 5 kV. SEM-energy dispersive spectroscopy (EDS) mapping was performed using a QuantaX200 (Bruker). X-ray photoelectron spectroscopy (XPS, Thermo Scientific, K-Alpha) was conducted using an Al X-ray source. The UV-vis analysis was performed on a V770 spectrophotometer (JASCO). The pH of the solution was measured by using a JENWAY 3510 pH Meter.
9
Characterization of Hb-AuNCs Nanostructures
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UV–Vis absorption spectrometer (JASCO V-770 Spectrophotometer, Easton, MD, USA) was applied to measure the absorption of Hb-AuNCs. XPS (VG Microtech MT-500, London, United Kingdom) with Al Kα x-ray source was used to analyze the oxidation state and binding properties of Hb-AuNCs. A fluorescence spectrometer (JASCO spectrofluorometer FP-8500, Easton, MD, USA) was utilized to detect the fluorescence of Hb-AuNCs. The Systat PeakFit 4.12 (Systat Software, San Jose, CA, USA) software was employed to simulate fluorescence spectra. TEM (Hitachi HT-7700, Tokyo, Japan) was applied to characterize the morphology of the Hb-AuNCs. FTIR spectroscopy (Thermo Scientific Nicolet iS10, Waltham, MA, USA) was utilized to measure the amide groups of Hb-AuNCs.
10
Spectrophotometric Determination of UO2(LX) Complexes
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Sample solutions of H2LX (X = NH, O, S, 0.1 mM) were prepared by mixing 3-tert-butyl-5-methoxysalicylaldehyde and the corresponding diamines in ethanol. The formation of H2LX (X = NH, O, S) was checked by 1H NMR spectroscopy (see Fig. S1† ). Triethylamine (NEt3, 0.4 mM) was added as a H+ scavenger after the formation of UO2(LX). The total concentration of UO22+ was stepwise increased up to 0.12 mM (0 ≤ [UO22+]/[LX2−] ≤ 1.2) by adding a feed solution of UO2(NO3)3·6H2O (10 mM) in ethanol. The UV-vis absorption spectrum at each increment step was recorded by JASCO V-770 spectrophotometer. During the whole titration experiment, temperature of the sample solution was kept at 293 K in a thermostat cell holder equipped with the spectrophotometer. The obtained titration series of the UV-vis absorption spectra was analyzed by HypSpec (version 1.1.33)30 (link) to determine conditional stability constants of UO2(LX) (X = NH, O, S) under the presence of 0.4 mM NEt3 in ethanol.
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