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2 protocols using hesperetin

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Phytochemical standards purification and quantification

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Acetonitrile, methanol, ethanol, silymarin, glucose, and fructose were purchased from Sigma Aldrich (St. Louis, MO, USA), and 4–hydroxyquinoline, salicylic acid, vanillic acid, rutin, trans–4–hydroxycinnamic acid, ellagic acid, sinapinic acid, luteolin, trans–ferulic acid, hesperidin, naringin, 4–hydroxybenzoic acid, and 3,4–dimethoxycinnamic acid (purity > 98%) were purchased from Aladdin (Shanghai, China). Dihydroxybenzoic acid, methyl syringate, fisetin, abscisic acid, quercetin, trans–cinnamic acid, naringenin, apigenin, kaempferol, hesperetin, kaempferide, daidzein, and chrysin (purity > 98%) were obtained from J&K Scientific (Beijing, China). Formic acid was purchased from Merck (Darmstadt, Germany).
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Polyphenol Standards Characterization and Analysis

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Polyphenol standards contain quercetin, naringenin, naringin, hesperidin, hesperetin, phloridzin, proanthocyanidin, gallic acid, luteolin, epigallocatechin-3-gallate (EGCG), quinic acid, ferulic acid, epigallocatechin (EGC), epicatechin (EC), chlorogenic acid, and caffeic acid, HPLC grade acetonitrile and 2,2-azinobis(3-ethylbenzthiazoline-6-sulphonate) (ABTS) were purchased from J&K Scientific Co. Ltd. (Beijing, China). Folin-Ciocalteu's phenol reagent, pepsin (porcine gastric mucosa, ≥400 units per mg protein), pancreatin (porcine pancreas, 4 USP specifications), lipase and bile salt and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carbonsaure (Trolox) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). All other chemicals were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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