Clone mrq 22

Manufactured by Cell Marque

Sourced in United States

Clone MRQ-22 is a laboratory-grade monoclonal antibody produced by Cell Marque. Its core function is to serve as a reagent for immunohistochemical staining procedures.

Automatically generated - may contain errors

Lab products found in correlation

Novared substrate kit, by Vector Laboratories (1 mentions) Bond max system, by Leica (1 mentions) Clone d1l2g, by Cell Signaling Technology (1 mentions) Immpress hrp universal antibody polymer, by Vector Laboratories (1 mentions) Ultra 5 block, by Thermo Fisher Scientific (1 mentions) Bond polymer refine detection kit, by Leica (1 mentions) E1l3n, by Cell Signaling Technology (1 mentions) Clone c8 144b, by Agilent Technologies (1 mentions) Clone e1l3n, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

MRQ-22 (2 citations) PD-1 (clone MRQ-22, (2 citations)

2 protocols using clone mrq 22

Immunohistochemical Analysis of PD-L1, PD-1, and VISTA

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Immunohistochemistry was carried out with antibodies directed against PD-L1 [dilution 1:100, E1L3N, Cell Signaling, Danvers, USA (catalog #13684)], PD-1 [dilution 1:100; clone MRQ-22, Cell Marque, Rocklin, USA (#315M-96)], and VISTA [1:500; clone D1L2G, Cell Signaling (#64953)]. Immunostaining of PD-L1 and PD-1 was performed with the autostainer Bond™ Max System (Leica Microsystems GmbH, Wetzlar, Germany). The immunoreaction was visualized with the Bond™ Polymer Refine Detection Kit [brown labeling; Novocastra; Leica Microsystems GmbH, Wetzlar, Germany (#DS9800)]. Immunostaining of VISTA was performed manually: Following antigen retrieval in citrate buffer (pH6), specimens were incubated with hydrogen peroxide block and Ultra V Block [both Thermo Scientific, Braunschweig, Germany (TA-125-HP and TP-125-HL)] to avoid unspecific reactions. The immunoreaction was visualized with the ImmPRESS-HRP-Universal–Antibody Polymer and the NovaRED substrate kit [both VectorLabs, Peterborough, United Kingdom (#SK-4800)]. Counterstaining was carried out with hematoxylin [Dr. K. Hollborn & Söhne GmbH & Co KG; Leipzig, Germany (#88663)].
Germinal centers of lymph follicles served as internal positive control for PD-L1 and PD-1.

Schoop H., Bregenzer A., Halske C., Behrens H.M., Krüger S., Egberts J.H, & Röcken C. (2019). Therapy Resistance in Neoadjuvantly Treated Gastric Cancer and Cancer of the Gastroesophageal Junction is Associated with an Increased Expression of Immune Checkpoint Inhibitors—Comparison Against a Therapy Naïve Cohort. Translational Oncology, 13(2), 165-176.

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Immunohistochemical Analysis of PD-1, PD-L1, and CD8+ Cells

Cited 1 time

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Immunohistochemical analysis was performed to investigate PD-1 and PD-L1 expression and the presence of CD8 + lymphocytes in the tumor. Tonsil (PD-L1 + , PD-1 + , and CD8 +) and appendix (CD8 + and PD1 +) served as positive controls. Immunohistochemistry was performed on 4-µm-thick FFPE sections of AS TMAs with one or two cores per sample from representative tumor areas (core size 2 mm) to allow simultaneous examination of patient specimens under identical conditions. Staining was performed in the Lab Vision Autostainer 360 (Thermo Fisher Scientific) by using the EnVision FLEX, pH high Link kit (Dako), and monoclonal rabbit anti-PD-L1 (1:800, clone E1L3N, Cell Signaling Technology), monoclonal mouse anti-PD-1 (1:20, clone MRQ-22, Cell Marque) or monoclonal mouse anti-CD8 (1:80, clone C8/144B, Dako).
PD-L1 expression on the tumor cells was scored as 0% ( −), 1–10% (+ / −), 10–50% ( +) or ≥ 50% positive tumor cells (+ +). All CD8 and PD-1 positive T cells were counted and subdivided in three categories: < 10 ( −), 10–50 ( +), or ≥ 50 positive cells (+ +) per tumor core [36 (link)].
Digital images were generated with VisionTekTM (Sakura, version 2.6) and analyzed at × 20 magnification.

Tomassen T., Weidema M.E., Hillebrandt-Roeffen M.H., van der Horst C., Desar I.M., Flucke U.E, & Versleijen-Jonkers Y.M. (2022). Analysis of PD-1, PD-L1, and T-cell infiltration in angiosarcoma pathogenetic subgroups. Immunologic Research, 70(2), 256-268.

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