Flaming brown microelectrode puller

Manufactured by Sutter Instruments

Sourced in United States

The Flaming/Brown microelectrode puller is a laboratory instrument designed to create fine-tipped glass micropipettes or microelectrodes. It utilizes a controlled heating and pulling process to produce these specialized tools for various scientific applications.

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Lab products found in correlation

Grass s88 stimulator, by Natus (1 mentions) Matlab, by MathWorks (1 mentions) Axoclamp 2b, by Molecular Devices (1 mentions) Leica vt1000, by Leica Microsystems (1 mentions)

3 protocols using flaming brown microelectrode puller

Electrophysiological Recording of Larval Drosophila Neuromuscular Junction

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Wandering, third-instar larvae were collected from the sides of their culture vials and then placed immediately onto a dissecting dish containing a modified hemolymph-like (HL3.1) Drosophila saline (pH 7.15)[29 (link)]. Intracellular recordings were obtained with sharp microelectrodes, pulled from thin-wall monofilament glass (WPI) using a Flaming-Brown microelectrode puller (P-97, Sutter Instrument), and filled with 3M KCl. Recordings of compound EJPs were made from longitudinal body wall muscles 6/7 within abdominal segments 3, 4, and 5. Potentials were recorded with an AxoClamp 2B (Molecular Devices) using custom DAQ routines composed in Matlab (Mathworks). Synaptic potentials were elicited by stimulating segmental motor neurons via a glass suction electrode, a Grass S88 stimulator, and a stimulus isolation unit (Grass Technologies, West Warwick, RI). Single impulses were generated at 0.2 Hz, 330 μs pulse duration, and ~120% of the voltage needed to attain maximal compound EJP amplitude.

Scarpelli E.M., Trinh V.Y., Tashnim Z., Krans J.L., Keller L.C, & Colodner K.J. (2019). Developmental expression of human tau in Drosophila melanogaster glial cells induces motor deficits and disrupts maintenance of PNS axonal integrity, without affecting synapse formation. PLoS ONE, 14(12), e0226380.

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Whole-cell Voltage Clamp of Amygdalar Neurons

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Whole-cell voltage clamp and field potential electrophysiological experiments were carried out in 4–5 week old male ICR mice as described previously (Patel et al., 2009 (link); Sumislawski et al., 2011 (link)). Briefly, Mice were anesthetized with isoflurane and transcardially perfused with ice-cold high sucrose, low Na⁺-containing ACSF. Following decapitation, the brain was removed and a 3mm coronal block of the amygdala was cut using an ice-chilled, coronal brain matrix. Thereafter, coronal slices (200–300μm) were made using a Leica VT1000S vibratome (Leica Microsystems, Bannockburn, IL) in a 1–4°C oxygenated (95% v/v O₂, 5% v/v CO₂) ACSF comprised of (in mM): 208 sucrose, 2.5 KCl, 1.6 NaH₂PO₄, 1 CaCl₂·2H₂O, 4 MgCl₂·6H₂O, 4 MgSO₄·7H₂O, 26 NaHCO₃, 1 ascorbate, 3 Na-pyruvate, and 20 glucose. Whole-cell voltage-clamp recordings were performed on CeAL neurons easily identified visually by their medium-sized, spherical somata. Patch electrodes were pulled on a Flaming/Brown microelectrode puller (Sutter Instruments) and filled with solution containing (in mM): 120 K⁺-gluconate, 4 NaCl, 10 HEPES, 20 KCl, 4 Mg-ATP, 0.3 Na-GTP, and 10 Na-phosphocreatine (pH 7.25–7.35, adjusted with KOH). eEPSCs were recorded from CeAL neurons via local microstimulation, ~100μm from the cell soma. Experimental details are described in the supplemental materials.

Ramikie T.S., Nyilas R., Bluett R.J., Gamble-George J.C., Hartley N.D., Mackie K., Watanabe M., Katona I, & Patel S. (2014). Multiple Mechanistically Distinct Modes of Endocannabinoid Mobilization at Central Amygdala Glutamatergic Synapses. Neuron, 81(5), 1111-1125.

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Electrophysiological Characterization of Engineered Pacemaker Cells

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The electrophysiological properties of genetically programmed pacemaker cells were measured via single-cell patch clamping and funny channel density two weeks after the initiation of transfection. Whole cell voltage-clamp experiments were carried out using the standard patch-clamp method [58 (link)]. Thin-walled borosilicate glass (1.5-mm, No. 7052, Garner Glass, Claremont, CA, USA) was used for pulling recording electrodes, using a Flaming/Brown microelectrode puller (P-97, Sutter Instruments, Novato, CA, USA), which were heat polished before use. The pipettes filled with internal solution had a tip resistance of 2–5 MΩ. Recordings were performed with an Axoclamp 2B patch-clamp amplifier (Axon Instruments, Foster City, CA, USA). The data filtered at 2 kHz were processed with Clampex 8 software (Axon Instruments). For current-clamp recordings, the intercellular solution contained 10 mM NaCl, 130 mM potassium aspartate, 0.04 mM CaCl₂, 3 mM Mg-ATP, and 10 mM HEPES. The pH was adjusted to 7.2 with KOH. The extracellular bath solution contained 140 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl₂, 1 mM MgCl₂, 10 mM glucose, and 5 mM HEPES at pH 7.4.

Karimi T., Pan Z., Potaman V.N, & Alt E.U. (2023). Conversion of Unmodified Stem Cells to Pacemaker Cells by Overexpression of Key Developmental Genes. Cells, 12(10), 1381.

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