Cd69 pe antibodies

Manufactured by Thermo Fisher Scientific

The CD69-PE antibody is a laboratory reagent used for the detection and analysis of CD69 expression on cell surfaces. CD69 is an early activation marker expressed on various immune cell types. The CD69-PE antibody is conjugated with the fluorescent dye Phycoerythrin (PE) to enable flow cytometric detection and quantification of CD69-positive cells.

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Lab products found in correlation

Mitosox red, by Thermo Fisher Scientific (2 mentions) Phospho erk, by Cell Signaling Technology (2 mentions) N galactosamine galn, by Merck Group (2 mentions) Nec 1s 7 cl o nec 1, by Abcam (2 mentions) Nfatc2, by Santa Cruz Biotechnology (2 mentions) Ripk1, by Santa Cruz Biotechnology (2 mentions) Anti gapdh antibody mab374, by Merck Group (2 mentions) Phospho p38α, by Merck Group (2 mentions) Cd4 percp cy5, by Thermo Fisher Scientific (2 mentions) Tumor necrosis factor (tnf), by Thermo Fisher Scientific (2 mentions) Hdac1, by Santa Cruz Biotechnology (2 mentions) Anti fas clone jo2, by BD (2 mentions) Cd3 fitc, by BD (2 mentions) Mdivi 1, by Enzo Life Sciences (2 mentions) Phospho drp1 ser 637, by Cell Signaling Technology (1 mentions) Ripk3, by Santa Cruz Biotechnology (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) Phospho drp1, by Cell Signaling Technology (1 mentions) Cd56 apc, by Thermo Fisher Scientific (1 mentions) Cd57 fitc, by Thermo Fisher Scientific (1 mentions)

3 protocols using cd69 pe antibodies

Investigating Cell Death Pathways

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The reagents and sources were as follows: Con A, LPS from Escherichia coli O111:B4, and N-galactosamine (GalN) (Sigma-Aldrich); TNF (Peprotech); α-galactosyl ceramide (α-GalCer, KRN7000) and Mdivi-1 (Enzo Life Sciences); Nec-1s (7-Cl-O-Nec-1, BioVision); FK 506 (LC Labs); cycloheximide (Calbiochem); phospho-ASK1(sc-109911, 1:500 dilution), NFATc2 (sc-7296, 1:1000 dilution), RIPK1 (sc-7881, 1:1000 dilution), RIPK3 (sc-47364, 1:1000 dilution), and HDAC1 (sc-7872, 1:500 dilution) antibodies (Santa Cruz Biotechnology); anti-GAPDH antibody (MAB374, 1:5000 dilution, Chemicon); phospho-p38α (#9211), phospho-JNK (#9251), phospho-ERK (#9101), and phospho-Drp1 (Ser637, #4867) antibodies (1:1000 dilution, Cell Signaling Technology); anti-MLKL antibody (AP14272b, 1:1000 dilution, Abgent); anti-Fas (clone Jo2, #554254, 5 μg per mouse, BD Pharmingen); CD3-FITC (#11-0032, 0.25 μg per 10⁵ cells), CD4-PerCP/Cy5.5 (#35-0042, 0.125 μg per 10⁵ cells), and CD69-PE antibodies (#1-0691, 0.25 μg per 10⁵ cells) (eBioscience); and MitoSOX RED (Molecular Probes). APC-mCD1d/PBS57 ligand tetramers were generously provided by the National Institute of Allergy and Infectious Disease MHC Tetramer Core Facility (Atlanta, GA, USA).

Kang Y.J., Bang B.R., Han K.H., Hong L., Shim E.J., Ma J., Lerner R.A, & Otsuka M. (2015). Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling. Nature Communications, 6, 8371.

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Mechanistic Study of Apoptosis Regulation

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The reagents and sources were as follows: Con A, LPS from Escherichia coli O111:B4, and N-galactosamine (GalN) (Sigma-Aldrich); TNF (Peprotech); α-galactosyl ceramide (α-GalCer, KRN7000) and Mdivi-1 (Enzo Life Sciences); Nec-1s (7-Cl-O-Nec-1, BioVision); FK 506 (LC Labs); cycloheximide (Calbiochem); phospho-ASK1(sc-109911, 1:500 dilution), NFATc2 (sc-7296, 1:1,000 dilution), RIPK1 (sc-7881, 1:1,000 dilution), RIPK3 (sc-47364, 1:1,000 dilution), and HDAC1 (sc-7872, 1:500 dilution) antibodies (Santa Cruz Biotechnology); anti-GAPDH antibody (MAB374, 1:5,000 dilution, Chemicon); phospho-p38α (#9211), phospho-JNK (#9251), phospho-ERK (#9101) and phospho-Drp1 (Ser637, #4867) antibodies (1:1,000 dilution, Cell Signaling Technology); anti-MLKL antibody (AP14272b, 1:1,000 dilution, Abgent); anti-Fas (clone Jo2, #554254, 5 μg per mouse, BD Pharmingen); CD3-FITC (#11–0032, 0.25 μg per 10⁵ cells), CD4-PerCP/Cy5.5 (#35–0042, 0.125 μg per 10⁵ cells), and CD69-PE antibodies (#1–0691, 0.25 μg per 10⁵ cells) (eBioscience); and MitoSOX RED (Molecular Probes). APC-mCD1d/PBS57 ligand tetramers were generously provided by the National Institute of Allergy and Infectious Disease MHC Tetramer Core Facility (Atlanta, GA, USA).

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Multicolor Flow Cytometric Analysis of Immune Cell Activation

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After 18 h incubation with IL-2 alone, IL-2 with LPS, or IL-2 with IL-12 (10 ng/ml; Sigma-Aldrich), immunofluorescent staining was performed using CD56-APC, CD57-FITC, and CD69-PE antibodies (eBioscience) followed by multicolor flow cytometric analysis.

Kanevskiy L.M., Erokhina S.A., Streltsova M.A., Telford W.G., Sapozhnikov A.M, & Kovalenko E.I. (2014). Bacterial Lipopolysaccharide Activates CD57-Negative Human NK Cells. Biochemistry. Biokhimiia, 79(12), 1339-1348.

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