25 μL of isooctane suspended total milk lipid was taken to dryness, were resuspended in 200 μL dichloromethane containing 15 μL of a 20% nonaethylene glycol monododecyl ether (Sigma Aldrich, St. Louis, MO) dissolved in dichloromethane (wt/vol), and samples were incubated for 5 minutes at 25°C. Samples were taken to dryness at 40°C for 25 minutes ensuring solvent was completely evaporated. Pellets containing triglyceride/nonionic surfactant complexes were reconstituted in 200 μL of ultrapure water without mixing and incubated at 40°C for 10 minutes, followed by a gentle vortex. A regression curve was prepared from 80 nmol of tripalmitin (Sigma Aldrich, St. Louis MO) combined with 25 μL of 20% nonaethylene glycol monododecyl ether in dichloromethane (wt/vol), processed as above, resuspended in 100 μL of ultrapure water, and a standard curve of 20, 10, 5, 2.5, 1.25, 0.625, and 0.3125 nmol tripalmitin was made. Triglyceride from the organic fraction was quantified relative to tripalmitin using a modified colorimetric assay [13 (link)]. Triglyceride Reagent and Free Glycerol Reagent were purchased from Sigma Aldrich (St. Louis, MO) and prepared according to the manufacturer's instructions.
Tripalmitin
Manufactured by Merck Group
Sourced in United States
Tripalmitin is a type of lipid molecule that is commonly used in the formulation of various laboratory reagents and products. It is a triglyceride composed of three palmitic acid molecules. Tripalmitin serves as a key component in the preparation of buffers, emulsions, and other laboratory materials where a stable lipid source is required.
Automatically generated - may contain errors
Lab products found in correlation
Nonaethylene glycol monododecyl ether, by Merck Group (3 mentions)
Free glycerol reagent, by Merck Group (3 mentions)
Tricaprin, by Merck Group (3 mentions)
Trilaurin, by Merck Group (3 mentions)
Triolein, by Merck Group (3 mentions)
Trimyristin, by Merck Group (3 mentions)
Diolein, by Merck Group (2 mentions)
Hplc grade acetonitrile, by Avantor (2 mentions)
Tag reagent, by Merck Group (2 mentions)
Tristearin, by Merck Group (2 mentions)
Polyvinyl alcohol (pva), by Merck Group (2 mentions)
Triglyceride reagent, by Merck Group (1 mentions)
Triton x 100, by Junsei (1 mentions)
Tributyrin, by Merck Group (1 mentions)
Hitrap q sepharose fast flow, by Cytiva (1 mentions)
Ammonium sulfate, by Junsei (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
P nitrophenyl palmitate p npp, by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
19 protocols using tripalmitin
1
Triglyceride Quantification in Milk Lipids
2
Isolation and Characterization of Chestnut Lipase
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Korean chestnuts (Castanea crenata) were purchased from local markets. They were peeled, sealed in a vacuum plastic bag, and stored at 4 °C for subsequent use. Before use, the chestnuts were covered with a layer of wet cotton and allowed to germinate for 3 days at 18 °C.
HiTrap DEAE Sepharose Fast Flow, HiTrap Q Sepharose Fast Flow, and HiPrep Sephacryl S-100 HR were purchased from Cytiva (Uppsala, Sweden). Trizma® base (≥ 99.9%), diethyl ether (≥ 99.9%), p-nitrophenyl palmitate (p-NPP), sodium dodecyl sulfate (SDS), isooctane (IOT), tributyrin, tricaproin, tricaprin, trilaurin, tripalmitin, and triolein (≥ 99.0%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ammonium sulfate (≥ 99.5%), Triton X-100, sodium chloride (≥ 99.5%), and agar were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Boric acid, potassium chloride, and oleic acid were obtained from Duksan Pure Chemicals Co., Ltd. (Ansan, Gyeonggi-do, Korea). The Costar® 96-well microplates (clear wall, clear bottom) used for the fluorometric assay were purchased from Corning Co. (Corning, NY, USA). All other chemicals were of analytical grade and were used without further purification.
HiTrap DEAE Sepharose Fast Flow, HiTrap Q Sepharose Fast Flow, and HiPrep Sephacryl S-100 HR were purchased from Cytiva (Uppsala, Sweden). Trizma® base (≥ 99.9%), diethyl ether (≥ 99.9%), p-nitrophenyl palmitate (p-NPP), sodium dodecyl sulfate (SDS), isooctane (IOT), tributyrin, tricaproin, tricaprin, trilaurin, tripalmitin, and triolein (≥ 99.0%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ammonium sulfate (≥ 99.5%), Triton X-100, sodium chloride (≥ 99.5%), and agar were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Boric acid, potassium chloride, and oleic acid were obtained from Duksan Pure Chemicals Co., Ltd. (Ansan, Gyeonggi-do, Korea). The Costar® 96-well microplates (clear wall, clear bottom) used for the fluorometric assay were purchased from Corning Co. (Corning, NY, USA). All other chemicals were of analytical grade and were used without further purification.
3
Enzymatic Lipid Modification Protocols
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AOL L03 (Aspergillus oryzae lipase powder) was purchased from Leveking Bioengineering Co.,Ltd. (Shenzhen, China). Lipozyme RM IM (Rhizomucor miehei immobilized on an ion-exchange resin), Lipozyme TL IM (Thermomyces lanuginosa immobilized on silica gel) and porcine pancreatic lipase (PPL) were purchased from Novozymes A/S (Bagsvaerd, Denmark). Macroporous resins AB-8, D3520, NKA were acquired from the Chemical Plant of NanKai University (Tianjing, China). Macroporous resins SD300, SD600, DM11, DM130, anion-exchange macroporous resins D354 FD, D314 FD, D318, and cation-exchange resin C258 FD were purchased from Zhejiang Zhengguang industrial Co., Ltd. (Hangzhou, China). Table 1 comparitively describes the properties of these materials. The standards of tripalmitin, C52 compound (primarily OPO and OPO isomeric compounds), diolein (mixed isomers) and monoolein were from Sigma-Aldrich (St Louis, USA). Palm stearin (tripalmitin 48.69%, C52 13.42% and sn-2 PA 27.18%) and OA (82.5%) were obtained from Xinshili Food Science Co., Ltd. (Nanjing, China) and Yihai Kerry Fine Chemical Co., Ltd (Lianyungang, China), respectively. Other reagents used were either HPLC or analytical reagent grades and obtained from various sources.
4
Rat Ghrelin Quantification Protocol
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Solvents and chemicals were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France) or Thermo Fisher Scientific (Elancourt, France). Tricaprylin was from TCI Europe (Zwijndrecht, Belgium). Tripalmitin was from Sigma. Rat plasma acylated and unacylated ghrelin enzyme immunoassays kits (EIA) were purchased from SPI-Bio (Montigny Le Bretonneux, France). Kits for plasma glucose, cholesterol and triglycerides were purchased from Bio-mérieux (Lyon, France). Rabbit anti-ghrelin polyclonal antibodies (bs-0467R) used for immunohistochemistry were purchased from Bioss (Massachusetts, USA).
5
Topical Formulations Characterization
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Icaridin 97% (ICA) was obtained from Chemidin (China). Geraniol 98% (GRL) was obtained from Quinarí (Ponta Grossa, Paraná, Brazil). Tripalmitin, polyvinyl alcohol (PVA), and hydroxypropylmethylcellulose (HPMC) were obtained from Sigma-Aldrich (St. Louis, United State). Myrytol was obtained from BASF. The ethanol used was from Labsynth (Diadema, São Paulo, Brazil). Acetonitrile (HPLC grade) was from J.T. Baker (United State). Other reagents were obtained from local suppliers in Sorocaba (São Paulo, Brazil).
6
Formulation and Characterization of Insect Repellent
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Ethyl butyl acetylamine propionate butylacetylaminopropionate 98% (IR3535) and icaridin 97% (ICA) was obtained from Chemidin (China). Geraniol 98% (GRL) was obtained from Quinarí (Ponta Grossa, Paraná, Brazil). Tripalmitin, polyvinyl alcohol (PVA), and poloxamer 407 (PL407) were obtained from Sigma-Aldrich (St. Louis, USA). Mirytol was obtained from BASF. Ethanol was purchased from Labsynth (Diadema, São Paulo, Brazil). Acetonitrile (HPLC grade) was obtained from J.T. Baker (USA). All other reagents were obtained from local suppliers in Sorocaba (São Paulo, Brazil).
7
Reagents and Solvents for Analytical Procedures
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Methanol (MeOH) and chloroform (CHCl3) were ultra-residue solvents of analytical grade purchased from Merck (Darmstadt, Germany). Sulfuric acid (95.5%), CaCl2, Na2CO3, CuSO4·5H2O, Na and K tartrate and PBS pH 7.4 (Na2HPO4 and NaH2PO4) were reagent grade (Sigma Aldrich Chemie, Munich, Germany). D-glucose, BSA, tripalmitin and Zarrouk components [21 ] were of reagent grade and purchased from Sigma Aldrich.
Double-deionized water with a conductivity of less than 18.2 MΩ was obtained with a Milli-Q system (Millipore, Bedford, MA, USA). The Folin–Ciocalteu reagent was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Double-deionized water with a conductivity of less than 18.2 MΩ was obtained with a Milli-Q system (Millipore, Bedford, MA, USA). The Folin–Ciocalteu reagent was purchased from Sigma-Aldrich (St. Louis, MO, USA).
8
Quantification of Milk Lipid Triglycerides
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Twenty-five µL of isooctane-suspended total milk lipid was evaporated to dryness, samples were resuspended in 200 µL dichloromethane containing 15 µL of a 10% nonaethylene glycol monododecyl ether (Sigma-Aldrich, St. Louis, MO) dissolved in dichloromethane (wt/vol). Samples were incubated for 5 min at 25 °C and taken to dryness at 40 °C for 25 min ensuring organic solvent was completely evaporated. Pellets contained triglyceride (TAG)/nonionic surfactant complexes, to which 200 µL of ultrapure water was carefully added without mixing and incubated at 40 °C for 10 min, followed by a gentle vortex. A standard regression curve 20, 10, 5, 2.5, 1.25, 0.625, and 0.3125 nmol was prepared from 80 nmol tripalmitin (Sigma-Aldrich) incubated with 25 µL of 10% nonaethylene glycol monododecyl ether, incubated, dried and resuspended in 100 µL of ultrapure water as above. Total TAG from the organic fraction was quantified using a modified colorimetric assay34 (link) and expressed as mM concentrations. TAG Reagent and Free Glycerol Reagent (Sigma-Aldrich) were diluted according to the manufacturer’s instructions.
9
Quantification of Milk Lipid Triglycerides
10
Triglyceride Substrate Specificity Assay
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Substrate specificity was studied by using triglyceride substrates; tributyrin (C4), tricaproin (C6), tricaprylin (C8), tricaprin (C10), trilaurin (C12), trimyristin (C14), tripalmitin (C16) and olive oil (C18) (Sigma) at 60 °C and pH 8.0.
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