Texas red dhpe

Manufactured by Thermo Fisher Scientific

Sourced in United States

Texas Red DHPE is a fluorescent lipid probe used to label and track cell membranes and lipid structures. It has an absorption maximum at 595 nm and an emission maximum at 615 nm, in the red region of the visible spectrum.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Texas Red (171 citations) Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE) (8 citations) Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red DHPE) (6 citations) Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (Texas Red-DHPE) (3 citations) Triethylammonium salt (Texas Red-DHPE) (3 citations) Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt, (TR-DHPE) (2 citations) Texas red-labeled 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (TR-DHPE), (2 citations)

32 protocols using texas red dhpe

Electroformation of Fluorescent GUVs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

GUVs were prepared via electroformation as essentially described^{28 (link),29 (link)}. Lipid-coated ITO slides were dried under vacuum overnight. The following lipid mixtures (in mol%) were spread on two ITO slides [the fluorescent lipid Texas Red-DHPE (Life Technologies) was added for imaging purposes]: DOPC/Texas Red-DHPE: 99.75/0.25, DOPC/TR-DHPE/cholesterol: 74.75/0.25/25, 94.75/0.25/5 DOPC/TR-DHPE/DOPS, 74.75/0.25/5/20 DOPC/TR-DHPE/DOPS/cholesterol, 59.75/0.25/20/20, DOPC/TR-DHPE/DOPS/cholesterol, 94.75/0.25/5 DOPC/TR-DHPE/DOPA, 74.75/0.25/5/20 DOPC/TR-DHPE/DOPA/cholesterol, DOPC/TR-DHPE/DOPS/cholesterol, 94.75/0.25/5 DOPC/TR-DHPE/PI(4,5)P₂, 74.75/0.25/5/20 DOPC/TR-DHPE/PI(4,5)P₂/cholesterol, 69.75/0.25/10/20 DOPC/TR-DHPE/PI(4,5)P₂/cholesterol or 69.75/0.25/10/20 DOPC/TR-DHPE/PI(3)P/cholesterol. The two ITO slides were combined with the space between filled with sucrose (10%, w/v) and incubated at RT for 1.5 h (electroformation). The GUVs were added at room temperature to DyLight488-labeled rCPn0473 (100 µg/ml) and immediately observed on a confocal fluorescence microscope (Nikon Eclipse Ti-E with A1R confocal laser scanner, 60x oil objective, NA = 1.49). Image acquisition and analysis was performed with NIS-Elements (Nikon).

Galle J.N., Fechtner T., Eierhoff T., Römer W, & Hegemann J.H. (2019). A Chlamydia pneumoniae adhesin induces phosphatidylserine exposure on host cells. Nature Communications, 10, 4644.

+ Open protocol

+ Expand

Purification of Vacuolar Trafficking Proteins

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lipids were obtained from Avanti Polar Lipids (Alabaster, AL), except for ergosterol which was from Sigma-Aldrich (St. Louis, MO), PI(3)P was from Echelon Biosciences (Salt Lake City, UT), and the fluorescent lipids (Marina-Blue-DHPE, NBD-DHPE, Rhodamine-DHPE, Oregon-Green-488-DHPE, Texas-Red-DHPE) were from Life Technologies (Carlsbad, CA). Biotinylated R-phycoerythrin was purchased from Life Technologies, Cy5-derivatized streptavidin from KPL (Gaithersburg, MD), and unlabeled streptavidin from Thermo Scientific (Waltham, MA). Sec18p (Haas and Wickner, 1996 (link)), Sec17p (Schwartz and Merz, 2009 (link)), Ypt7p (Zick and Wickner, 2013 (link)), HOPS (Zick and Wickner, 2013 (link)), and vacuolar SNARE proteins (Mima et al., 2008 (link); Schwartz and Merz, 2009 (link); Zucchi and Zick, 2011 (link)) were purified as described. Vti1p and Nyv1p were exchanged into octylglucoside buffer as described (Zucchi and Zick, 2011 (link)). Soluble Nyv1p (sR) was purified as GST-TEVsite-Nyv1(Δtm) as described (Thorngren et al., 2004 (link)), and cleaved with TEV protease prior to use. GST-His6-3Csite-Nyv1p was purified as described (Izawa et al., 2012 (link)).

Zick M, & Wickner W.T. (2014). A distinct tethering step is vital for vacuole membrane fusion. eLife, 3, e03251.

+ Open protocol

+ Expand

Phospholipid-Sterol Monolayer Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC), 1,2-didecanoyl-sn-glycero-3-phosphocholine (DDPC), dihydrocholesterol (DChol), and 27-hydroxycholesterol (27OH) were purchased from Avanti Polar Lipids (Alabaster, AL) and used as received. Texas Red DHPE was purchased from Life Technologies (Carlsbad, CA). Phospholipid/sterol ratio mixtures (70/30) were prepared in chloroform from stock solutions with 0.5 mol % Texas Red DHPE to provide contrast between the coexisting phases and those stored at −22 °C. A spreading solution concentration of 0.5 mg/mL was used for all experiments. The 70/30 phospholipid/sterol ratio is approximately the miscibility critical composition.¹² Finally, to minimize any effects on monolayer oxidation, we replaced cholesterol with dihydro-cholesterol, and all images and isotherms were taken within 1 h of monolayer deposition to reduce any photo-oxidative effects.^{35 (link),36}

Stottrup B.L., TigreLazo J., Bagonza V.B., Kunz J.C, & Zasadzinski J.A. (2019). Comparison of Line Tension Measurement Methods for Lipid Monolayers at Liquid−Liquid Coexistence. Langmuir : the ACS journal of surfaces and colloids, 35(48), 16053-16061.

+ Open protocol

+ Expand

Fluorescent Labeling of Lipid Rafts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The LSPs and CPPs were fixed with 2% paraformaldehyde (PFA) for 20 min, washed with TSS and incubated with 0.1 µmol/L Texas Red^® DHPE (Life Technologies, Grand Island, NY) for 30 min at RT. Samples were cytospun onto poly-L-lysine-coated glass slide, washed with PBS, mounted with ProLong Gold Antifade (Life Technologies) and imaged using LSM 700 (Carl Zeiss, Oberkochen, Germany) with 63x/1.40 oil objective (27 (link)).

Tegegn T.Z., De Paoli S.H., Orecna M., Elhelu O.K., Woodle S.A., Tarandovskiy I.D., Ovanesov M.V, & Simak J. (2016). Characterization of procoagulant extracellular vesicles and platelet membrane disintegration in DMSO-cryopreserved platelets. Journal of Extracellular Vesicles, 5, 10.3402/jev.v5.30422.

+ Open protocol

+ Expand

Fluorescent Labeling of Pseudoviruses

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For single-virus experiments, pseudoviruses were labeled with the fluorescent membrane label Texas Red-DHPE (Invitrogen) following the protocol we have previously used for influenza and Zika viruses (48 (link), 58 (link)). Texas Red solution (0.74 mg/mL) in ethanol was mixed with HM buffer (20 mM HEPES, 20 mM MES, 130 mM NaCl [pH 7.4]) at a ratio of 1:40. Fifty microliters of 100-fold-concentrated purified pseudovirus particles (total viral protein concentration of ~2 to 2.5 mg/mL for MLV pseudoviruses as measured via a BCA assay) was mixed with 200 μL of Texas Red-DHPE/HB (HEPES 20 mM, 150 mM NaCl [pH 7.2]) buffer suspension and incubated at room temperature in the dark for 2 h on a rocker. After that, 2.75 mL of HB buffer was added to the mixture, which was divided into two aliquots. Each aliquot was pelleted at 20,000 × g for 60 min at 4°C, resuspended in 25 μL of HM buffer at pH 7.4, stored at 4°C, and used within 1 week.

Sengar A., Cervantes M., Bondalapati S.T., Hess T, & Kasson P.M. (2023). Single-Virus Fusion Measurements Reveal Multiple Mechanistically Equivalent Pathways for SARS-CoV-2 Entry. Journal of Virology, 97(5), e01992-22.

+ Open protocol

+ Expand

Preparation of Fluorescent Lipid Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GUV formation procedure was adopted and modified from a previously published method.⁵¹ In a 2 mL vial, 40 μL of 10 mM lipid solution in chloroform was dried under N₂ to form a lipid film. 200 μL of light mineral oil was added to the vial and the mixture was sonicated at 60 °C for 1 h until the lipid was fully dissolved in the oil. In a 0.7 mL Eppendorf tube, 10 μL of the upper buffer (100 mM HEPES, 200 mM sucrose, 1 mM 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) in H₂O, pH = 7.4) was added to 100 μL of lipid solution. The mixture was flicked to form an emulsion. The emulsion was gently layered on top of 100 μL of lower buffer (100 mM HEPES, 200 mM glucose in H₂O, pH = 7.4) in a different tube and the mixture was centrifuged at 10 000 rcf for 5 min. Light mineral oil was removed by vacuum suction and the vesicle solution was obtained. 0.1 μL of 100 μM Texas Red DHPE (Invitrogen, Carlsbad, CA) solution in EtOH was added to 10 μL of vesicle solution prior to microscope observation.

Zhou C.Y., Wu H, & Devaraj N.K. (2015). Rapid access to phospholipid analogs using thiol-yne chemistry †Electronic supplementary information (ESI) available: Details of experimental procedures, lipid characterization data (NMR, HRMS, steady-state fluorescence anisotropy), HPLC analysis, fluorescence microscopy images, liposome shrinkage data in the form of AVI files. See DOI: 10.1039/c5sc00653h Click here for additional data file.. Chemical Science, 6(7), 4365-4372.

+ Open protocol

+ Expand

Lipid Monolayer Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ultrapure water (resistivity ≥18.2 megohm·cm) from a Milli-Q Direct-Q 3 UV-R system (MilliPore) was used as the subphase for all experiments. 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (r-DPPC, R-enantiomer) (Avanti Polar Lipids, Alabaster, AL) or 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (rac-DPPC) (MilliporeSigma, Germany) was mixed with PA (MilliporeSigma, Germany) or 1-HD (MilliporeSigma, Germany) in various molar ratios with small mole fractions of dihydrocholesterol (DChol) (MilliporeSigma, Germany) in chloroform solution. Dihydrocholesterol was used instead of cholesterol to minimize oxidation but has little impact on monolayer morphology or phase behavior ((5 )). Fluorescence contrast was achieved via the addition of 0.75 mol % Texas Red DHPE [N-(Texas Red sulfonyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine] (Invitrogen, Grand Island, NY). The Texas Red-DHPE is excluded from L_C domains and segregates to L_E domains, providing image contrast. Replacing the insoluble Texas Red DHPE dye with soluble Rhodamine 123 in the subphase does not change the fingering instabilities or the circle-to-stripe transition (fig. S12) (59 (link)).

Valtierrez-Gaytan C., Barakat J.M., Kohler M., Kieu K., Stottrup B.L, & Zasadzinski J.A. (2022). Spontaneous evolution of equilibrium morphology in phospholipid-cholesterol monolayers. Science Advances, 8(14), eabl9152.

+ Open protocol

+ Expand

Fluorescent ATP Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

2Na-ATP was purchased from AppliChem (Darmstadt, Germany). Neomycin and spermine were from Sigma (St Louis, MO). Alexa Fluor 647-labeled ATP and BODIPY TR-labeled ATP were from Invitrogen (Carlsbad, CA). All other chemicals were reagent grade and obtained from standard suppliers. All lipids are from Avanti Polar lipids (Alabaster, AL) except of Oregon Green-DHPE and Texas Red-DHPE (Invitrogen).

Park Y., Seo J.B., Fraind A., Pérez-Lara A., Yavuz H., Han K., Jung S.R., Kattan I., Walla P.J., Choi M., Cafiso D.S., Koh D.S, & Jahn R. (2015). Synaptotagmin-1 binds to PIP2-containing membrane but not to SNAREs at physiological ionic strength. Nature structural & molecular biology, 22(10), 815-823.

+ Open protocol

+ Expand

Lipid Vesicle Preparation with Fluorescent Probes

Check if the same lab product or an alternative is used in the 5 most similar protocols

The composition of lipid vesicles is egg-PC (Avanti) mixed with 0.5 mol % of Texas Red-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (Texas Red-DHPE) (Invitrogen) and 1 mol % of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(biotinyl) (Biotin-DOPE) (Avanti). Lipids were dissolved in chloroform and mixed in a fixed molar ratio as described above. The lipid solution was then blown dried in clean glass tubes with pure nitrogen followed by vacuum drying overnight to remove the remaining chloroform. The dried lipid was stored in −20 C° to prevent oxidization. When preparing lipid vesicles, 1 mg of the lipid mixture was suspended in 400 μL of PBS buffer and extruded through a 100 nm Nucelpore membrane filter with the use of an Avanti polar lipid extruder and Hamilton syringes as the extruder device. The lipid vesicle solution is stored in 4 °C for up to 1 month.

Lou H.Y., Zhao W., Hanson L., Zeng C., Cui Y, & Cui B. (2017). Dual-Functional Lipid Coating for the Nanopillar-Based Capture of Circulating Tumor Cells with High Purity and Efficiency. Langmuir : the ACS journal of surfaces and colloids, 33(4), 1097-1104.

+ Open protocol

+ Expand

Lipid Vesicle Preparation and Characterization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lipids DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine), DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), and DSPE-Bio-PEG2000 (distearoylphosphatidylethanolamine – N - (biotinyl (polyethylene glycol) 2000)) were obtained from Avanti Polar Lipids (Alabaster, AL). Texas Red® DHPE (Texas Red® 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt) was obtained from Invitrogen/Life Technologies (Grand Island, NY).
For preparation of large unilamellar vesicles (LUVs)⁴⁵, a lipid mixture containing 45% DOPS, 30% DOPE and 25% DOPC was first dried with compressed air and then desiccated at least 2 hours before rehydration. The protein storage buffer was used to rehydrate lipids at a concentration of 1 mg/ml. Rehydrated lipids were vortexed occasionally at room temperature for 1 hour. Next, the dispersions were extruded 13 times through a single polycarbonate membrane of 400 nm pore size (Whatman/GE Healthcare). The resulting LUVs were stored at 4°C and used within two days.
Giant unilamellar vesicles (GUVs) containing 1) 45%DOPS, 30%DOPE, 24.5%DOPC and 0.5% TexasRed DHPE and 2) 64%DOPC, 25%DOPG, 10% PI(4,5)P2, 0.3% TexasRed DHPE and 0.7% DSPE-Bio-PEG2000 were electroformed in 300 mM sucrose as described in refs^{31 (link),32 (link),45 ,46 (link)}.

Chen Z., Zhu C., Kuo C.J., Robustelli J, & Baumgart T. (2016). The N-terminal amphipathic helix of Endophilin does not contribute to its molecular curvature generation capacity. Journal of the American Chemical Society, 138(44), 14616-14622.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!