Mouse mcpt 1 uncoated elisa kit

Mast cell activities were determined by the amounts of circulating MCPT-1 as described previously (40 (link)). The serum samples collected at the time of sacrifice were diluted 1:10 with the assay buffer, and 100 μL of the diluted samples were used to quantify MCPT-1 using the Mouse MCPT-1 Uncoated ELISA Kit (Thermo Fisher Scientific) according to the manufacturer's instructions.

The serum samples collected at the time of sacrifice were used at a 1:10 dilution to quantify histamine and MCPT-1 as indicators of systemic and brain MC activities. For histamine detection, a competitive histamine ELISA kit was used (Enzo Life Sciences, Inc., Farmingdale, NY, USA). The diluted terminal plasma samples (1:10) and 50 µg/mL of the brain lysates were used to determine histamine levels according to the manufacturer’s instructions. MCPT-1 was detected using a Mouse MCPT-1 Uncoated ELISA Kit according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA).

mMCPT-1 was detected using the MCPT-1 mouse uncoated ELISA kit (Thermo Fisher) following the manufacturer’s protocol. Histamine was assayed using Histamine EIA kit (Oxford Biomedical Research) according to the manufacturer’s protocol. For the peanut-specific IgE ELISA, sera from individual mice were added to peanut-coated Maxisorp immunoplates (Nalge Nunc). Peanut-specific IgE antibodies were detected with goat anti-mouse IgE-unlabelled (Southern Biotechnology) and rabbit anti-goat IgG-alkaline phosphatase (Invitrogen) and developed with p-nitrophenyl phosphate (SeraCare Life Sciences). For peanut-specific IgG1 ELISA, sera from individual mice were added to peanut-coated Maxisorp immunoplates (Nalge Nunc). Peanut-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugated (Southern Biotechnology Associates) and TMB liquid substrate system for ELISA (Sigma Aldrich). The plates were read in an ELISA plate reader at 405 nM (IgE) or 450 nm (IgG1). Optical density values were converted to nanograms per millilitre of IgE or IgG1 by comparison with standard curves of purified IgE or IgG1 using linear regression analysis and are expressed as the mean concentration for each group of mice ± s.e.m.