Victor x4 multilabel plate reader

Manufactured by PerkinElmer

Sourced in United States, Germany, Canada, Finland

The Victor X4 Multilabel Plate Reader is a versatile laboratory instrument designed for high-throughput detection and analysis of various assays. It is capable of performing multiple detection modes, including absorbance, fluorescence, and luminescence measurements, to support a wide range of applications in life science research and drug discovery.

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Lab products found in correlation

Human instant elisa kit, by Thermo Fisher Scientific (5 mentions) Sample diluent, by Thermo Fisher Scientific (4 mentions) Europium solution, by PerkinElmer (4 mentions) Plus reagent, by Thermo Fisher Scientific (3 mentions) Dual luciferase reporter assay system, by Promega (3 mentions) Sulfinpyrazone, by Merck Group (3 mentions) Opteia human elisa kit, by BD (3 mentions) Delfia assay buffer, by PerkinElmer (3 mentions) Delfia enhancement solution, by PerkinElmer (3 mentions) In vitro toxicology assay kit, by Merck Group (3 mentions) 1 step ultra tmb substrate solution, by Thermo Fisher Scientific (3 mentions) 2450 microbeta2 scintillation counter, by PerkinElmer (3 mentions) Batda reagent, by PerkinElmer (3 mentions) Human elisa kit, by Thermo Fisher Scientific (2 mentions) Lipofectamine, by Thermo Fisher Scientific (2 mentions) Lance camp 384 kit, by PerkinElmer (2 mentions) Europium labelled streptavidin, by PerkinElmer (2 mentions) Cyquant cell proliferation assay kit, by Thermo Fisher Scientific (2 mentions) Delfia cell proliferation kit, by PerkinElmer (2 mentions) Delfia dna fragmentation assay, by PerkinElmer (2 mentions)

104 protocols using victor x4 multilabel plate reader

Cellular Proliferation and Viability Assays

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Proliferation rate was evaluated using 5-bromo-2′-deoxyuridine (BrdU). This assay was performed on fixed cells, incubated (one hour, 37 °C, 5% CO₂) with europium labelled anti-BrdU antibody and visualized at wavelength OD 340 nm using VICTOR™ X4 Multilabel Plate Reader (Perkin Elmer). The mitochondrial redox activity was estimated with Presto reagent (Invitrogen). Cell viability was measured spectrophotometrically at a wavelength of 570 nm every 10 min on a VICTOR™ X4 Multilabel Plate Reader (Perkin Elmer).

Kałuzińska Ż., Kołat D., Kośla K., Orzechowska M., Bednarek A.K, & Płuciennik E. (2021). In vitro and in silico assessment of the effect of WWOX expression on invasiveness pathways associated with AP-2 transcription factors in bladder cancer. BMC Urology, 21, 36.

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Nanodiamonds Impact on Cell Viability

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Cell viability was measured using CCK-8 assay (Dojindo Molecular Technologies Inc., Japan) which corresponds to WST-8 tetrazolium salt to assess mitochondrial dehydrogenase activity. Fao cells were seeded at a density of 2000 cells/well on 96-well plates and were allowed to attach overnight. Media was aspirated and replaced with ND-conditioned media containing 10, 25, 50 and 100 µg•mL -1 of NDs. At each predetermined time point (days 2, 4 and 7), cells were washed with PBS once and 100 μL of fresh media containing 10% CCK-8 reagent was added to each well. After three hours of incubation in the dark, the media was transferred to a new 96 well plate, and the optical density (OD) of each well was measured using a microplate reader at 450 nm (Victor x4 multilabel plate reader, Perkin Elmer, USA).
For DNA quantification, media was removed at day 2, 4 and 7 and each well was washed with PBS once followed by addition of 75 μL of CyQUANT NF® assay dye reaction mix (ThermoFisher Scientific, Australia). Plates were incubated in the dark for 45 min before measuring fluorescence at excitation and emission wavelengths of 485 and 535 nm respectively using microplate reader (Victor X4, multilabel plate reader, Perkin Elmer, USA).

Khanal D., Zhang F., Song Y., Hau H., Gautam A., Yamaguchi S., Uertz J., Mills S., Kondyurin A., Knowles J.C., Knowles J.C., Georgiou G., Ramzan I., Cai W., Ng K.W, & Chrzanowski W. (2019). Biological impact of nanodiamond particles - label free, high-resolution methods for nanotoxicity assessment. Nanotoxicology, 13(9).

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Plasma MPO Quantification by ELISA

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Quantitative determination of plasma MPO concentration was performed by Human Instant ELISA kit (eBioscience, San Diego, CA, USA). Preliminary plasma dilution 1:100 was performed for each sample with specific Sample Diluent (eBioscience, San Diego, CA, USA). MPO determination was performed according to the manufacturer’s protocol and instructions. Optical density was read by using a VICTORX4 Multilabel Plate Reader (PerkinElmer Life Sciences, Waltham, MA, USA) at 450 nm. The levels of this molecule were calculatedfrom the standard curve based on the manufacturer’s protocol. Standard samples ranged from 0.16–10.0 ng/mL. Human MPO Instant ELISA Kit sensitivity is 0.03 pg/mL. All tests were performed in triplicate.

Clementi A., Virzì G.M., Milan Manani S., Battaglia G.G., Ronco C, & Zanella M. (2022). Eryptosis in Patients with Chronic Kidney Disease: A Possible Relationship with Oxidative Stress and Inflammatory Markers. Journal of Clinical Medicine, 11(23), 7167.

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Quantitative Determination of Cu/ZnSOD in Plasma

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Quantitative determination of Cu/ZnSOD concentration in plasma samples was performed by Human ELISA kit (eBioscience, San Diego, CA, USA). Preliminary plasma dilution 1:20 was performed for each sample with specific Sample Diluent (eBioscience, San Diego, CA, USA). Cu/ZnSOD determination was performed based on manufacturer’s protocol and instructions. Optical density was read by using a VICTORX4 Multilabel Plate Reader (PerkinElmer Life Sciences, Waltham, MA, USA) at 450 nm. The levels of these molecules were calculated from the standard curve according to the manufacturer’s protocol. Standard samples ranged from 0.08–5.0 ng/mL. Human Cu/ZnSODInstant ELISA Kit sensitivity is 0.04 ng/mL. All the tests were performed in triplicate.

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Quantitative IL-6 Determination in Plasma

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Quantitative determination of IL-6 in plasma samples was performed by Human Instant ELISA kit (eBioscience, San Diego, CA, USA). Cytokine determination was performed according to manufacturer’s protocol and instructions. Optical density was read by using a VICTORX4 Multilabel Plate Reader (PerkinElmer Life Sciences, Waltham, MA, USA) at 450 nm. The levels of this molecule were calculated from standard curves, according to the manufacturer’s protocol. All the tests were performed in triplicate. Standard samples for IL-6 ranged from 3.1 to 200 ng/mL and the sensitivity of this test was 0.92 ng/mL.

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Anthrax Immune Globulin Preparation

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Purified recombinant native protective antigen [83 kDa (1 μM=83μg/ml); PA83] was obtained from BEI Resources (Manassas, VA). Activated protective antigen [63 kDa (1 μM=63μg/ml); PA63] and recombinant lethal factor [90 kDa (1 μM=90 μg/ml); LF] were obtained from List Biological Laboratories (Campbell, CA). Dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA) buffer, wash concentrate, enhancement solution, Streptavidin Microtitration 96-well plates, Platewash, Plateshake, and Victor™ X4 Multilabel Plate Reader were from Perkin-Elmer Life Sciences (Shelton, CT). Lethal toxin (LTx) was prepared by combining PA63 and LF in a 7:4 ratio. Anthrax Immune Globulin (AIG), an investigational product for anthrax treatment consisting primarily of anti-PA antibody, was previously acquired from Cangene (Winnipeg, MB, Canada).

Stoddard R.A., Quinn C.P., Schiffer J.M., Boyer A.E., Goldstein J., Bagarozzi D.A., Soroka S.D., Dauphin L.A, & Hoffmaster A.R. (2014). Detection of anthrax protective antigen (PA) using europium labeled anti-PA monoclonal antibody and time-resolved fluorescence. Journal of immunological methods, 408, 78-88.

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Assessing IDH2 Mutant Effects on Cell Proliferation and Migration

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MCF10A^P and MCF10^H1047R cells were transfected with pCMV6-TagRFP empty vector, pCMV6-TagRFP-IDH2^WT or pCMV6-TagRFP-IDH2^R172S using Lipofectamine 3000 (Invitrogen) as per manufacturer’s instructions. After 48 hours, cells were trypsinized, collected and RFP-, RFP-IDH2^WT, and RFP-IDH2^R172S, expressing cells selected by flow cytometry sorting. For proliferation, the sorted cells were seeded in 96, well plates (1,000 cells/well) in triplicates. Cell growth was assessed every 24 h by CellTiter-Blue assay (Promega) and fluorometric detection performed with 560 nm excitation and 590 nm emission using a VICTOR X4 Multilabel Plate Reader (PerkinElmer). For migration, cells were starved for 16 h in 2% horse serum DMEM/F12 medium without EGF. Cells were seeded (0.5 × 10⁵) in the upper compartment of a 24-well transwell insert (8 μm pore membranes; Corning) and allowed to adhere and migrate at 37°C towards 10% horse serum and EGF containing DMEM/F12 medium for 16 h. Cells adherent to the upper side of the transwell membrane were removed, and cells on the inferior side of were fixed in 4% paraformaldehyde (PFA), permeabilized (0.5% Triton X-100 in PBS), and stained with crystal violet (0.3%). Transwell membranes were imaged (EVOS XL Imaging System, Life Technologies) and analyzed by ImageJ.

Chiang S., Weigelt B., Wen H.C., Pareja F., Raghavendra A., Martelotto L.G., Burke K.A., Basili T., Li A., Geyer F.C., Piscuoglio S., Ng C.K., Jungbluth A.A., Balss J., Pusch S., Baker G.M., Cole K.S., von Deimling A., Batten J.M., Marotti J.D., Soh H.C., McCalip B.L., Serrano J., Lim R.S., Siziopikou K.P., Lu S., Liu X., Hammour T., Brogi E., Snuderl M., Iafrate A.J., Reis-Filho J.S, & Schnitt S.J. (2016). IDH2 Mutations Define a Unique Subtype of Breast Cancer with Altered Nuclear Polarity. Cancer research, 76(24), 7118-7129.

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Luciferase Assay for PPAR-γ Activation

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HG5LN-zfLxr and HG5LN-zfPparγ cells were seeded at 40,000 cells per well in 96-well white opaque tissue culture plates, and maintained in DMEM supplemented with 5% FCS. Twenty-four hours later, culture medium was replaced with phenol-red and serum-free DMEM containing T0 or GW (GW3965, Tocris, Bristol, UK), and cells were incubated with the ligands for 16 hours. At the end of the incubation, culture medium was replaced with fresh medium containing 0.3 mM luciferin, and luciferase activity was measured for 2 seconds using the Victor X4 Multilabel Plate Reader (Perkin Elmer, Waltham, MA). Nonlinear regression curve fit was used to plot the dose response curves using GraphPad Prism version 5 (GraphPad Software, La Jolla, CA). Experiments were performed in quadruplicate and repeated three times.

Pinto C.L., Kalasekar S.M., McCollum C.W., Riu A., Jonsson P., Lopez J., Swindell E., Bouhlatouf A., Balaguer P., Bondesson M, & Gustafsson J.Å. (2015). Lxr regulates lipid metabolic and visual perception pathways during zebrafish development. Molecular and cellular endocrinology, 419, 29-43.

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Quantifying Osteoblast Gene Expression

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MC3T3-E1 cultures at >90% confluency were co-transfected with Firefly Luciferase reporter constructs and SV40-Renilla using Lipofectamine (Invitrogen) and Plus Reagent (Invitrogen) according to manufacturer’s instructions. The SV40-Renilla construct serves to control for slight variability in transfection efficiency between plates. A total of 2.5 μg of plasmid DNA (2 μg:0.5 μg, test: reference) was transfected per 60 mm plate. After 4 days post-differentiation, cultures were harvested and reporter activities were measured using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI) on a VICTOR X4 Multilabel Plate Reader (Perkin Elmer, Waltham, MA), according to manufacturers’ instructions. Each test condition described is represented by three biological replicates, each assayed in duplicate. Activities of test promoter constructs between pre-osteoblasts and differentiating osteoblasts were further normalized to the activity of the TK promoter construct that was transfected in parallel cultures. Statistical significance values assessed by Student’s t-test are reported where applicable.

Tai P.W., Wu H., Gordon J.A., Whitfield T.W., Barutcu A.R., van Wijnen A.J., Lian J.B., Stein G.S, & Stein J.L. (2014). Epigenetic landscape during osteoblastogenesis defines a differentiation-dependent Runx2 promoter region. Gene, 550(1), 1-9.

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Cell Proliferation Assay for AQ1, AN6, and AQ7

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MCF7 and HCG27 cells were seeded at concentrations comprised between 0.3×10⁴ and 0.5×10⁴ cells/well in a 96-well flat bottom plate (Sarstedt Italia, Verona, Italy). After 24 hours, AQ1 or AN6 were added at concentrations from 0.01 μM up to 10 μM for 72 hours. Additional wells exposed either to the vehicle (DMSO, 0.1% final concentration) or to medium alone were prepared, too. At the end of the experiment, 20 μL of CellTiter-Blue^® Cell Viability Assay (Alamar Blue, Promega, Madison, USA) were added to each well and the fluorescence was measured at 560 nm as excitation wavelength and 590 nm as emission wavelength, by using a VICTOR^™X4 Multilabel Plate Reader (Perkin Elmer, Waltham, USA). Three separate experiments were executed and each concentration was tested in sestuplicate. In line with preliminary comparative in vitro studies (data not shown), the sulforhodamine B (Sigma-Aldrich Co., St. Louis, USA) assay was used to measure the effect of AQ7 on cell proliferation. Both cell lines were exposed to a range of concentrations up to 10 μM for 0, 24, 48, 72, and 96 hours. Three separate experiments were executed, and each concentration was tested in sestuplicate.

Zorzan E., Ros S.D., Musetti C., Shahidian L.Z., Ramos Coelho N.F., Bonsembiante F., Létard S., Gelain M.E., Palumbo M., Dubreuil P., Giantin M., Sissi C, & Dacasto M. (2016). Screening of candidate G-quadruplex ligands for the human c-KIT promotorial region and their effects in multiple in-vitro models. Oncotarget, 7(16), 21658-21675.

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