Calf thymus dna

Manufactured by Rockland Immunochemicals

Calf thymus DNA is a purified preparation of deoxyribonucleic acid (DNA) extracted from the thymus gland of calves. It is a natural source of double-stranded DNA that can be used as a reference material or in various research applications.

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Lab products found in correlation

Multiskan fc elisa plate reader, by Thermo Fisher Scientific (2 mentions) α mouse igm or igg antibody, by Southern Biotech (1 mentions) α mouse igm or igg, by Southern Biotech (1 mentions) P nitrophenyl phosphate, by Thermo Fisher Scientific (1 mentions) Lps from escherichia coli, by Merck Group (1 mentions)

2 protocols using calf thymus dna

Quantifying Mouse Immunoglobulins and Autoantibodies

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100 µl of mouse blood samples were collected from living mice by a transverse incision through the major tail vein, and incubated in an Eppendorf tube containing heparin, the samples were centrifuged for 15min at 1400 rpm at 4°C. For the anti-IgM and anti-IgG ELISAs, 96-well plates (NUNC, maxisorp) were coated with polyclonal anti-mouse IgM or IgG antibody (SouthernBiotech) and blocked with buffer containing 1% BSA. Dilutions of anti-mouse IgM/IgG (SouthernBiotech) were used as standard. The concentration of IgM and IgG in the supernatants was determined by detection with alkaline-phosphatase-labeled anti-mouse IgM or IgG (Southern Biotech), respectively. P-nitrophenylphosphate (Genaxxon) in diethylamine buffer was added and data were acquired at 405 nm using a Multiskan FC ELISA plate reader (Thermo Scientific). To determine the content of autoreactive anti-dsDNA specific antibodies, concentration-adjusted cell culture supernatants were applied to plates coated with calf thymus DNA (Rockland Immunochemicals).

Vadakumchery A., Faraidun H., Ayoubi O.E., Outaleb I., Schmid V., Abdelrasoul H., Amendt T., Khadour A., Setz C., Göhring K., Lodd K., Hitzing C., Alkhatib A., Bilal M., Benckendorff J., Al Shugri A.K., Brakebusch C.H., Engels N., Datta M., Hobeika E., Alsadeq A, & Jumaa H. (2022). The Small GTPase RHOA Links SLP65 Activation to PTEN Function in Pre B Cells and Is Essential for the Generation and Survival of Normal and Malignant B Cells. Frontiers in Immunology, 13, 842340.

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Analysis of Blimp-1 Impact on Splenic B Cell IgM and Autoantibody Production

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Mature splenic B cells from the indicated genotypes were cultured in the presence of 2.5 μg/mL LPS from Escherichia coli (0111:B4; Sigma-Aldrich) for 1.5 days and subjected to retroviral transduction either with pMIG (EV) or pMIG-Blimp-1, respectively. The supernatants were collected 5 days after transduction and used for α-IgM and α-dsDNA-IgM-ELISA.
For the α-IgM and α-IgG ELISAs 96-well plates (NUNC, maxisorp) were coated with polyclonal α-mouse IgM or IgG antibody (SouthernBiotech) and blocked with buffer containing 1% BSA. Dilutions of α-mouse IgM/IgG (SouthernBiotech) were used as standard. The concentration of IgM and IgG in the supernatants was determined by detection with alkaline-phosphatase-labeled α-mouse IgM or IgG (Southern Biotech), respectively. P-nitrophenylphosphate (Genaxxon) in diethylamine buffer was added and data were acquired at 405 nm using a Multiskan FC ELISA plate reader (Thermo Scientific). To determine the content of autoreactive IgM antibodies, IgM-concentration-adjusted cell culture supernatants were applied to plates coated with calf thymus DNA (Rockland Immunochemicals). The detection and development was performed as described for the α-IgM ELISA.

Setz C.S., Hug E., Khadour A., Abdelrasoul H., Bilal M., Hobeika E, & Jumaa H. (2018). PI3K-Mediated Blimp-1 Activation Controls B Cell Selection and Homeostasis. Cell Reports, 24(2), 391-405.

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