Cd44 antibody

Manufactured by Proteintech

The CD44 antibody is a laboratory tool used to detect and study the CD44 protein. CD44 is a cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. The antibody can be used in various applications, such as flow cytometry, immunohistochemistry, and Western blotting, to identify and characterize cells expressing the CD44 protein.

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Lab products found in correlation

Cd44 pe, by Thermo Fisher Scientific (1 mentions) Snai1, by Cell Signaling Technology (1 mentions) Cd105, by BD (1 mentions) Cd146, by BD (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Cd104, by Thermo Fisher Scientific (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-CD44 antibody (3 citations) Anti-CD44 polyclonal antibody (2 citations)

2 protocols using cd44 antibody

Expression of Stemness and EMT Markers

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Antibodies against Vimentin (Cat#5741), SNAI1 (Cat #3879), ZEB1 (Cat #3396), SOX2 (Cat #3579), E-Cadherin (Cat #14472), TWIST1(Cat #46702), Ki67(Cat #9129) were purchased from Cell Signal Technology. Antibody against EBV LMP1 was purchased from Abcam (Cat #ab78113). Antibody against EBV LMP2A was purchased from Santa Cruz Biotechnology (Cat #sc-101314). CD44 PE (Cat #12-0441-81), CD104 (Cat #PA5-79541), CD104 eFluor 660 (Cat #50-1049-82) antibodies were purchased from ThermoFisher Scientific. CD44 antibody was purchased from Proteintech (Cat #15675-1-AP). Antibodies against CD90 (Cat #555596), CD146 (Cat #550315), CD105 (Cat #560839), CD166 Antibody (Cat #560903) were purchased from BD Bioscience.

Zhu N., Xu X., Wang Y., Zeng M.S, & Yuan Y. (2021). EBV latent membrane proteins promote hybrid epithelial-mesenchymal and extreme mesenchymal states of nasopharyngeal carcinoma cells for tumorigenicity. PLoS Pathogens, 17(8), e1009873.

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Flow cytometric analysis of SMSC markers

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The surface markers of the SMSCs were analyzed by utilizing flow cytometric analysis. SMSCs passaged for 2 times were harvested and washed in PBS. SMSCs (1.0 x 10⁶) were then incubated with CD73 antibody (cat.12231-1-AP; 1:500 dilution, Proteintech), CD45 antibody (cat. 60287-1-Ig; 1:500 dilution, Proteintech), CD44 antibody (cat.12231-1-AP; 1:500 dilution, Proteintech), CD90 antibody (cat. 65088-1-Ig; 1:500 dilution, Proteintech), and CD105 antibody (cat. 10862-1-AP; 1:500 dilution, Proteintech) for at last 90 min at 37 °C and analyzed using flow cytometry.

Hu B., Zou X., Yu Y., Jiang Y, & Xu H. (2023). METTL3 promotes SMSCs chondrogenic differentiation by targeting the MMP3, MMP13, and GATA3. Regenerative Therapy, 22, 148-159.

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