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2 protocols using phloroglucinol

1

Analytical Standards for Phytochemical Analysis

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Analytical grade chemicals, including sodium hydroxide, methanol, hydrochloric acid, sodium acetate, acetone and phloroglucinol, were purchased from the Beijing chemical plant (Beijing, China). Chromatographic-grade solvents, including methanol and acetonitrile, were acquired from Honeywell (Marris Township, NJ, USA), and formic acid (≥99%) was purchased from ROE Scientific (Newark, NJ, USA). The authentic standards of malvidin-3-O-glucoside, quercetin-3-O-glucoside, catechin, (−)-epicatechin, (−)-epigallocatechin, and (−)-epicatechin-3-O-gallate were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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2

Comprehensive Analysis of Polyphenols

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NaOH, HCl, methanol (analytical grade), phloroglucinol, and NaAc were supplied from Beijing Chemical Works. Acetonitrile, formic acid, and methanol (HPLC grade) were supplied from Fisher (Fairlawn, NJ, USA). C, EC, EGC, ECG, quercetin-3-O-glucoside, and malvidin-3-O-glucoside were purchased from Sigma-Aldrich (St. Louis MO, USA). Volatile standards listed in Supplementary Table S1, PVPP, and n-alkanes were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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