Ctx elisa kit

Aliquots of 1 µL randomly selected from the different media were used for total protein evaluations (n=4). Total protein concentrations were measured using the Protein A280 Nanodrop™ 1/Onec Microvolume UV-Vis Spectrophometer (Thermo Scientific, Wilmington, DE, USA) at 280 nm. Matrix degradation by MMPs was measured by the quantity of solubilized type I collagen C-terminal cross-linked telopeptides (ICTP), using 2 aliquots of 100 µL from different media and groups (n=4) and the ICTP ELISA kit (MyBiosource Inc, San Diego, USA). To determine matrix degradation by cysteine cathepsins, the amount of solubilized C-terminal peptide (CTX) was quantified using 3 aliquots of 100 µL from different media and groups (n=4) and the CTX ELISA kit (MyBiosource Inc, San Diego, USA). The average ratios of ICTP and CTX in relation to total protein concentration (ICTPtp and CTXtp) were calculated.^{16 (link)} Mean values for ICTPtp and CTXtp were analyzed by 1-way ANOVA followed by Tukey’s post-hoc test. For all tests, the level of significance was set at 0.05.