B6 cg tg gfap cre ert2 505fmv j

All experimental procedures have followed the guidelines of the National Institutes of Health and approved by the Animal Care and Use Committee at the Institute of Neuroscience, Chinese Academy of Sciences. Brain slice experiments were performed using young mice (only male mice were used, ~3 weeks at the time of virus injection). In vivo experiments were performed on adult mice (including both genders, > 6 weeks at the time of data collection). Mice were housed in a 12/12-h light/dark cycle (light on at 7 AM) with food and water available ad libitum. Mice with implants for EEG/EMG recording, optogenetic manipulation, or fiber photometry recording were housed individually. WT mice (C57BL/6) were purchased from institute-approved vendors (Shanghai Silaike or LingChang Experiment Animal Co., China). VGAT-IRES-Cre (Slc32a1^tm2(cre)Lowl/J, #016962), VGLUT2-IRES-Cre (Slc17a6^tm2(cre)Lowl/J, # 016963) and CD73-KO mice (B6.129S1-Nt5e^tm1Lft/J, #018986), GFAP-Cre/ERT2 mice (B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J, #012849), GFAP-Cre (FVB-Tg(GFAP-cre)25Mes/J, #004600), Aldh1l1-Cre (B6;FVB-Tg(Aldh1l1-Cre)JD1884Htz/J, #023748) were obtained from The Jackson Laboratory. IP₃R₂^flox mice were kindly shared by Kunfu Ouyang from Peking University. GFAP-Cre mice were back-crossed to C57BL/6J mice for at least four generations to reduce the FVB strain contribution before usage.

The GFAP-Cre^ERT2:Rosa26^iDTR mouse line was generated by crossing the B6.Cg-Tg (GFAP-cre/ERT2)505Fmv/J (Jackson Laboratories, Bar Harbor, ME, USA, strain #012849) mouse with the Rosa26:iDTR (Jackson Laboratories, Bar Harbor, ME, USA, strain #007900) mouse (Figure 1). Males and females hemizygous for both alleles were utilized in all experiments. Animals were 6–12 weeks of age at the start of all experiments.