As described in ref. 64 (link), for the implantation of a head-post and optical chamber, the animals were anesthetized with gas anesthesia (Isoflurane 1.5–2.5%; Pfizer) and injected with an antibiotic (Baytril, 0.5 ml, 2%; Bayer Yakuhin), a steroidal anti-inflammatory drug (Dexamethasone; Kyoritsu Seiyaku), an anti-edema agent (Glyceol, 100 μl; Chugai Pharmaceutical) to reduce brain swelling, and a painkiller (Lepetan, Otsuka Pharmaceutical). The scalp and periosteum were retracted, exposing the skull, and then a 5.5 mm diameter trephination was made with a micro drill (Meisinger LLC). Two 5 mm coverslips (120-170 μm thickness) were positioned in the center of the craniotomy in direct contact with the meninges, topped by a 6 mm diameter coverslip with the same thickness. When needed, Gelform (Pfizer) was applied around the 5 mm coverslip to stop any bleeding. The 6-mm coverslip was fixed to the bone with cyanoacrylic glue (Aron Alpha, Toagosei). A round metal chamber (7.1 mm diameter) combined with a head-post was centered on the craniotomy and cemented to the bone with dental adhesive (Super-Bond C&B, Sun Medical), which was mixed with a black dye for improved light absorbance during imaging.
Gelform
Manufactured by Pfizer
Sourced in United States
Gelform is a laboratory equipment product designed for use in various research and testing applications. It serves as a versatile gel-forming material, allowing for the creation of stable, three-dimensional structures within controlled environments.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using gelform
1
Cranial window implantation
2
Cranial Implant for Optical Imaging
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Implantation of a head-post and optical chamber. Animals were anesthetized with gas anesthesia (Isoflurane 1.5–2.5%; Pfizer) and injected with an antibiotic (Baytrile, 0.5 ml, 2%; Bayer Yakuhin), a steroidal anti-inflammatory drug (Dexamethasone; Kyoritsu Seiyaku), an anti-edema agent (Glyceol, 100 µl, Chugai Pharmaceutical) to reduce swelling of the brain, and a painkiller (Lepetan, Otsuka Pharmaceutical). The scalp and periosteum were retracted, exposing the skull, then a 4 mm diameter trephination was made with a micro drill (Meisinger LLC). A 4 mm coverslip (120–170 µm thickness) was positioned in the center of the craniotomy in direct contact with the brain, topped by a 6 mm diameter coverslip with the same thickness. When needed, Gelform (Pfizer) was applied around the 4 mm coverslip to stop any bleeding. The 6 mm coverslip was fixed to the bone with cyanoacrylic glue (Aron Alpha, Toagosei). A round metal chamber (6.1 mm diameter) combined with a head-post was centered on the craniotomy and cemented to the bone with dental adhesive (Super-Bond C&B, Sun Medical), mixed to a black dye for improved light absorbance during imaging.
3
Retrograde Labeling of Retinal Ganglion Cells
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To evaluate whether E-LPs affected RGC survival, retrograde labeling of RGCs with a retrograde fluorescent tracer Fluoro-gold (FG) was performed in a manner similar to that described previously.23 (link),25 Briefly, rats were anesthetized with 5% isoflurane, then maintained in 2.5% isoflurane. Skin of the head was incised in the midline to expose the skull and sutured (sagittal, coronal, and lambdoid sutures). The incisions were subjected to bilateral 2 mm diameter craniotomies at 0.5 mm posterolateral to the sagittal and lambdoid sutures. Superior colliculi were carefully exposed by removal of the cerebral content. A small piece of sterile sponge (gelform; Pfizer, Pearl River, NY, USA), presoaked in 10 µl of 4% FG solution, was left on the surface of the superior colliculus. After the surgery, the rats were kept warm and allowed to recover. Seven days after the surgery, the rats were euthanized and the eyes were enucleated and fixed with SuperFix (Kurabo, Osaka, Japan) for 2 hours at 4°C. Retinae were removed from the sclerae and divided into four quadrants (superior, inferior, nasal, and temporal) and mounted on slides. Fluorescence images of each quadrant at 2.0 mm from the optic nerve head were acquired and analyzed by counting the number of FG-labeled RGCs using the Multi Wavelength Cell Scoring Module of MetaMorph software (Molecular Devices) to avoid measurement bias.
4
Nanaomycin K Anti-Tumor Effects in Mice
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Animal experiments using a mouse bladder cancer model were done to investigate the anti-tumor effects of nanaomycin K. Male 6–8 week-old BALB/c nu/nu mice were purchased from CLEA Japan, Inc (Tokyo, Japan). 1 × 106 cells were inoculated at day 0 (n = 4, respectively) with Matrigel (Corning, Corning, NY). Mice were randomly assigned to 2 treatment groups (0.5 mg/body and 1.0 mg/body of nanaomycin K) and control groups (DMSO). Each dose of nanaomycin K was intratumorally administered with Gelform (Pfizer, New York, NY). Tumor volume was expressed by the following formula: (longest diameter) × (shortest diameter)2 × 0.5. Nine days after treatment, mice were sacrificed and tumors were collected10 (link).
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