All flow cytometric analyses and sorting were performed on a BD FACS Aria II cell sorter. Antibodies used were CD4 (RM4-5, BD Biosciences), CD8a (53-6.7, eBioscience), B220 (RA3-6B2, Biolegend), CD11b (M1/70, BD Biosciences), Ly6G/C (RB6-8C5, Biolegend), Ter119 (TER-119, Biolegend), Sca1 (D7, Biolegend), MPL (AMM2, Immuno-Biological Laboratories), CD41 (MWReg30, BD Biosciences), CD150 (TC15-12F12.2, Biolegend), cKit (2B8, Biolegend), CD34 (RAM34, eBioscience), IL7R (A7R34, eBioscience), Flt3 (A2F10, eBioscience), CD16/32 (93, eBioscience) and CD105 (MJ7/18, Biolegend). Streptavidin (eBioscience) was used to resolve biotinylated antibodies and propidium iodide was added prior to analysis to identify live cells.
Cd105 mj7 18
Manufactured by BioLegend
Sourced in United States
CD105 (MJ7/18) is a laboratory reagent used for the detection and analysis of CD105, also known as endoglin, in research applications. CD105 is a transmembrane glycoprotein that plays a role in transforming growth factor beta (TGF-β) signaling and is expressed on various cell types, including endothelial cells, smooth muscle cells, and some tumor cells. This reagent can be used in techniques such as flow cytometry to identify and characterize cells expressing CD105.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 rm4 5, by BD (1 mentions)
B220 ra3 6b2, by BioLegend (1 mentions)
Cd11b m1 70, by BD (1 mentions)
Cd150 tc15 12f12.2, by BioLegend (1 mentions)
Cd41 mwreg30, by BD (1 mentions)
Il 7r a7r34, by Thermo Fisher Scientific (1 mentions)
C kit 2b8, by BioLegend (1 mentions)
Sca1 d7, by BioLegend (1 mentions)
Ter119 ter 119, by BioLegend (1 mentions)
Cd8a 53 6, by Thermo Fisher Scientific (1 mentions)
Cd34 ram34, by Thermo Fisher Scientific (1 mentions)
Ly 6g c rb6 8c5, by BioLegend (1 mentions)
Cd16 32 93, by Thermo Fisher Scientific (1 mentions)
Facsaria 2 cell sorter, by BD (1 mentions)
Elementsadvanced research image analysis software, by Nikon (1 mentions)
Vegfr2 d5b1, by Cell Signaling Technology (1 mentions)
2 protocols using cd105 mj7 18
1
Multiparametric Flow Cytometry Analysis
2
Quantifying Angiogenesis and Immune Infiltrates
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For immunofluorescence, frozen tissue sections 5-um thick were stained with anti-mouse CD31 (Clone 390; BD Pharmingen, Franklin lakes, NJ, USA), CD105 (MJ7/18; Biolegend), VEGFR2 (D5B1; Cell Signaling Technology, Danvers, MA, USA). Texas Red and AF488 conjugated antibodies were used as secondary reagents. Staining in at least 10 fields per section was quantified with Nikon Elements Advanced Research Image Analysis software. Data are expressed as the area occupied by CD31 or VEGFR2 positive cells. For safety studies, 5um thick paraffin-embedded kidney and lung sections were analyzed for tumor-infiltrating lymphocytes by H&E staining as previously described (32 (link)).
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