The antibodies anti-RAB18 (11308-1-AP), anti-PLIN2 (15294-1-AP), anti-HSPA8 (10654-1-AP), and anti-NPC1 (13926-1-AP) were purchased from Proteintech; anti-LAMP1 (#9091), anti-GM130 (#12480), and anti-RELA (#3033) were purchased from Cell Signaling Technology; anti-LAMP2A (ab18528) was purchased from Abcam; anti-HA (A01244) and anti-His6 (C157) were purchased from Genscript; anti-FLAG (F1804) and anti-ATCB (A1978) were purchased from Sigma-Aldrich; anti-PRRSV-2 nucleocapsid (N) (SDOW17) was purchased from Rural Technologies; horseradish peroxidase (HRP)-conjugated donkey anti-mouse IgG (715-035-150) and anti-rabbit IgG (711-035-152) antibodies were purchased from Jackson ImmunoResearch Laboratories; anti-rabbit IgG antibodies labeled with Alexa Fluor 488 (A11034) and Alexa Fluor 568 (A11011) and anti-mouse IgG antibodies labeled with Alexa Fluor 488 (A11001), Alexa Fluor 568 (A11004), Alexa Fluor 405 (A31553), and Alexa Fluor Plus 647 (A32728) were purchased from Thermo Fisher Scientific. The antibodies described above were used at dilutions of 1:500 for immunofluorescence and 1:1,000 for immunoblot analysis.
Oil Red O (O0625) was purchased from Sigma-Aldrich. Chloroquine (HY-17589A) was purchased from MedChemExpress. BODIPY 493/503 (D3922) and DAPI (D1306) were purchased from Thermo Fisher Scientific.
Oil Red O (O0625) was purchased from Sigma-Aldrich. Chloroquine (HY-17589A) was purchased from MedChemExpress. BODIPY 493/503 (D3922) and DAPI (D1306) were purchased from Thermo Fisher Scientific.