The human CRC cell lines HCT116, RKO, LoVo, DLD‐1, SW480, and SW620 were obtained from the JCRB Cell Bank or ATCC. HCT116 p53(−/−) cells were purchased from Horizon Discovery. All cells were maintained in DMEM (Nacalai Tesque) supplemented with 10% FBS (Biowest, Nuaillé, France) and 1% penicillin–streptomycin (Nacalai Tesque). Cells were cultured at 37°C in a 5% CO2 incubator.
Sw480
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
The SW480 is a cell line derived from a human colorectal adenocarcinoma. It is a commonly used model for cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Dld 1, by Japanese Collection of Research Bioresources Cell Bank (5 mentions)
Hct116, by Japanese Collection of Research Bioresources Cell Bank (4 mentions)
Penicillin streptomycin, by Nacalai Tesque (3 mentions)
Colo201, by Japanese Collection of Research Bioresources Cell Bank (3 mentions)
Sw620, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Hct116 p53 cells, by Horizon Discovery (1 mentions)
Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Kmst 6, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Ccd 112con, by American Type Culture Collection (1 mentions)
Ls174t, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Lenti x 293t cells, by Takara Bio (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Du145, by RIKEN BioResource Center (1 mentions)
Nahco3, by Fujifilm (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
8 protocols using sw480
1
Maintenance of Human CRC Cell Lines
2
Culturing Human Cell Lines for Chemotaxis Assay
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The human cancer cell lines SW480, COLO201, COLO205 and the nontumorigenic immortal fibroblast human cell line KMST-6 were obtained from the Japanese Collection of Research Bioresources Cell Bank (Tokyo, http://cellbank.nibio.go.jp ). The human fibroblast cell line CCD-112CoN, which was derived from normal colon tissue, was acquired from the American Type Culture Collection (Manassas, VA, USA), and the other cell lines described below were from the Cell Resource Centre for Biomedical Research, Institute of Development, Aging and Cancer (Tohoku University, Sendai, Japan). All cancer cell lines were maintained at subconfluency in RPMI 1640, KMST-6 cells were cultured in MEM, and CCD-112CoN cells were cultured in EMEM. All culture media were supplemented with 10% foetal bovine serum, and the cells were maintained at 37°C in a humidified atmosphere containing 5% humidified CO2. The clear layers at the top of the medium were obtained and spotted onto assay plates to determine whether they induced a chemotactic response by C. elegans.
3
Culturing CRC Cell Lines for Research
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The human CRC cell lines HCT116, RKO, LS174T, LoVo, DLD‐1, SW480, and SW620 were obtained from the JCRB Cell Bank or the ATCC. Lenti‐X 293T cells were obtained from Takara for lentivirus production. Cells were cultured in DMEM with 10% FBS, 1% penicillin‐streptomycin (Nacalai Tesque), and 1% l ‐glutamine (Gibco). All cell lines were maintained at 37°C in a 5% CO2 incubator.
4
Culturing Various Cancer Cell Lines
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A549 (purchased from ATCC, Manassas, VA, USA) and PC14 (obtained from Dr. Hayata, Tokyo Medical College) cells were cultured in RPMI-1640 medium (Gibco Life Technologies Corp., Carlsbad, CA, USA). HeLa (obtained from National Cancer Center, Tokyo, Japan) and SBC-5 cells (obtained from Okayama University, Okayama, Japan) were cultured in Minimum Essential Medium (Gibco). A375 (purchased from ATCC), DU145 (purchased from RIKEN BRC, Tsukuba, Japan), MDA-MB-231 (purchased from ATCC), MIAPaCa2 (obtained from National Cancer Center), SAS (purchased from JCRB Cell Bank, Osaka, Japan), SW480 (purchased from JCRB Cell Bank), and U2OS (purchased from ATCC) cells were cultured in Dulbecco’s Modified Eagle’s Medium (Gibco). All media contained 0.2% NaHCO3 (Wako, Osaka, Japan), 10% FBS (Hyclone, Cytiva, Tokyo, Japan) and 1% penicillin–streptomycin (Invitrogen, Waltham, MA, USA). All cells were cultured at 37 °C in a humidified atmosphere maintained at 5% CO2.
5
Cell Culture Protocol for CRC, CACO2, and RKO
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The human CRC cell line SW480 was obtained from the JCRB cell bank; CACO2 cells were obtained from the RIKEN BioResource Research Center; and RKO cells were obtained from the ATCC. All cell lines were cultured in appropriate medium supplemented with 10% FBS at 37°C in an atmosphere containing 5% CO2.
6
Culturing Human Colorectal Cancer Cell Lines
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Human CRC cell lines HCT116, HCT116 p53(−/−), RKO, DLD‐1, and SW480 were obtained from the JCRB Cell Bank, ATCC, and Horizon Discovery. Cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin (Nacalai Tesque). All cell lines were maintained at 37°C in a 5% CO2 incubator.
7
Culturing CRC Cell Lines
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Human CRC cell lines CaR‐1, Colo320, Colo201, LoVo, SW480, and DLD‐1 were obtained from JCRB cell bank; Colo205 and HCT116 were obtained from RIKEN BioResource Research Center; and RKO, and SW620 were obtained from the ATCC. All cell lines were cultured in appropriate medium supplemented with 10% FBS at 37°C in an atmosphere containing 5% CO2.
8
Human Colorectal Cancer Cell Lines
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Ten cell lines derived from human CRC (DLD‐1, Colo‐201, RKO, WiDr, LoVo, HCT‐116, HT‐29, SW‐480, SW‐48 and SW‐837) were purchased from the Japanese Collection of Research Bioresources Cell Bank. All cell lines were maintained as described previously in a humidified atmosphere of 5% CO2 and 95% air at 37°C.8
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