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Multivision polymer detection system multivision anti rabbit ap anti mouse hrp polymers

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The Multivision Polymer Detection system is a laboratory equipment product from Thermo Fisher Scientific. It is designed to detect the presence of specific target proteins in biological samples using a polymer-based detection method. The system utilizes anti-rabbit/AP and anti-mouse/HRP polymer conjugates to enable simultaneous visualization of multiple target proteins in a single experiment.

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Lab products found in correlation

Antibody diluent, by Agilent Technologies (3 mentions) Kaiser s glycerol gelatin, by Merck Group (1 mentions) Ultravision lp detection system, by Thermo Fisher Scientific (1 mentions) Desmin, by Agilent Technologies (1 mentions)

Spelling variants of this product

HRP-anti-mouse (6 citations) MultiVision polymer detection system (anti-rabbit/HRP and anti-mouse/AP polymers; (2 citations)

4 protocols using multivision polymer detection system multivision anti rabbit ap anti mouse hrp polymers

1

Multivision Immunohistochemical Double Labeling

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Immunohistochemical double labeling was performed using the Multivision Polymer Detection system (MultiVision anti-rabbit/AP + anti-mouse/HRP polymers; Thermo scientific, Fremont, USA) according to the manufacturer’s instructions. For cryosections, Ultra V Block was supplemented with 10% human AB-serum.
Moser G., Weiss G., Gauster M., Sundl M, & Huppertz B. (2015). Evidence from the very beginning: endoglandular trophoblasts penetrate and replace uterine glands in situ and in vitro. Human reproduction (Oxford, England), 30(12), 2747-2757.
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2

Multivision Immunohistochemical Double Staining

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Immunohistochemical double staining was performed using the Multivision Polymer Detection system (MultiVision anti-rabbit/AP + anti-mouse/HRP polymers; Thermo scientific, Fremont, USA) according to the manufacturer’s instructions. Primary antibodies were diluted and mixed in antibody diluent (Dako, Carpinteria, USA), and this primary antibody cocktail was applied for 30 min at room temperature to the tissue sections. Table 1 lists details of all antibodies used and their respective dilutions. For this protocol, a hematoxylin counterstain is not recommended.
Moser G., Weiss G., Sundl M., Gauster M., Siwetz M., Lang-Olip I, & Huppertz B. (2016). Extravillous trophoblasts invade more than uterine arteries: evidence for the invasion of uterine veins. Histochemistry and Cell Biology, 147(3), 353-366.
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3

Multimarker Immunohistochemical Labeling

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Immunohistochemistry was performed using the Ultravision LP detection system (Thermo Scientific, Fremont, USA) according to the manufacturer's instructions. Interleucin-6 (mouse IgG1, 5 μg/ml, Peprotech, USA) and Desmin (mouse IgG1, 111 μg/ml, Dako, Vienna, Austria) were diluted in antibody diluent (Dako). Sections were counterstained with Mayer's hemalaun and mounted with Kaiser's glycerol gelatin (Merck, Vienna Austria). Negative controls were incubated with the appropriate IgG fractions as isotype controls.
vWF (rabbit IgG, 7.1 μg/ml, Sigma–Aldrich, USA) and HLAG (mouse IgG1, 1 μg/ml, Exbio, Czech Republic) were applied for immunohistochemical double labeling (Multivision Polymer Detection system (MultiVision anti-rabbit/AP + anti-mouse/HRP polymers; Thermo scientific, Fremont, USA) according to the manufacturer's instructions.
Gregor W., Berthold H., Siwetz M., Lang I, & Moser G. (2015). Arterial endothelial cytokines guide extravillous trophoblast invasion towards spiral arteries; an in-vitro study with the trophoblast cell line ACH-3P and female non-uterine endothelial cells. Placenta, 38, 49-56.
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4

Double Immunolabeling Reveals Fetal Plugs

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Immunohistochemical double labelling was performed using the MultiVision Polymer Detection system (MultiVision anti-rabbit/AP + anti-mouse/HRP polymers; Thermo scientific, Fremont, USA) according to the manufacturer’s instructions. Primary antibodies were diluted in antibody diluent (Dako). Binding of primary antibodies was visualized with the chromogens of the MultiVision kit, termed “LVBlue” and “LVRed”.
One specimen (week 11 of gestation) showed outstanding plug structures. This specimen was examined in detail.
Weiss G., Sundl M., Glasner A., Huppertz B, & Moser G. (2016). The trophoblast plug during early pregnancy: a deeper insight. Histochemistry and Cell Biology, 146(6), 749-756.
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