For immunofluorescence microscopy, the following antibodies were used: chicken anti-myc (1:300, A-21281; Molecular Probes, Eugene, OR), rabbit anti-SSX2IP (1:150, HPA027306; Sigma-Aldrich, Gillingham, United Kingdom), rabbit anti-PCM1 (1:300, sc67204; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti–γ-tubulin (1:250, T5192; Sigma-Aldrich), mouse anti–α-tubulin (1:250, T9026; Sigma-Aldrich), rabbit anti–centrin-2 (1:400, sc27793R; Santa Cruz Biotechnology), mouse anti-Plk4 (1:200, H00010733-B01; Abnova, Taipei, Taiwan), rabbit anti-Cep152 (1:400, A302-480A; Bethyl Laboratories, Montgomery, TX), rabbit anti-hSAS-6 (1:100, sc98506; Santa Cruz Biotechnology), mouse anti-centrobin (1:100, H00116840-B01P; Abnova), rabbit anti-Cep164 (1:200, 45330002; Novus Biologicals, Littleton, CO), mouse anti–C-Nap1 (1:100, 611374; BD Biosciences, San Jose, CA), and rabbit anti-CP110 (1:100, ab99337; Abcam, Cambridge, MA). Secondary antibodies were Alexa Fluor 488–coupled anti-rabbit, Alexa Fluor 594–coupled anti-rabbit, Alexa Fluor 594–coupled anti-mouse, Alexa Fluor 488–coupled anti-mouse, Alexa Fluor 488–coupled anti-chicken, and Cy3-coupled anti-mouse antibodies (all used at 1:1500). For immunoblotting, rabbit anti-SSX2IP (1:1000) and mouse anti–α-tubulin (1:5000) antibodies were used.
Hpa027306
Manufactured by Merck Group
Sourced in United Kingdom
HPA027306 is a piece of laboratory equipment manufactured by Merck Group. It is designed to perform a core function within the laboratory setting. Further details on the specific capabilities or intended use of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
T9026, by Merck Group (2 mentions)
Sc 27793 r, by Santa Cruz Biotechnology (1 mentions)
T5192, by Merck Group (1 mentions)
T5326, by Merck Group (1 mentions)
Ab4448, by Abcam (1 mentions)
Sc 50164, by Santa Cruz Biotechnology (1 mentions)
G7781, by Merck Group (1 mentions)
Glutathione sepharose bead, by GE Healthcare (1 mentions)
2 protocols using hpa027306
1
Immunofluorescence Microscopy Protocol
2
GST Pull-Down Assay with Detailed Antibodies
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GST pull-down was performed with Glutathione Sepharose beads (GE healthcare, Chalffint, UK) and carried out in low-salt EBC lysis buffer (150 mM NaCl; 50 mM Tris, pH 7.5; 1 mM EDTA; 0.5% NP40). Antibodies included in this study: CEP131 (A301-425A, Bethyl, WB 1:1000; ab84864, Abcam, Cambridge, UK, IF 1:300), PCM1 (sc-50164, Santa Cruz, IF 1:200), SSX2IP (HPA027306, Sigma-Aldrich, WB 1:1000), MK2 (3042S, Cell Signalling, Danvers, MA, USA, WB 1:1000), HSP27 pS82 (9709, Cell Signalling, WB 1:5000), GST (G7781, Sigma-Aldrich, WB 1:1000), Pericentrin (ab4448, Abcam, IF 1:1000), p38 MAPK (9212S, Cell Signalling, WB 1:1000), p38 MAPK phospho-Thr180/Tyr182 (9216S, Cell Signalling, WB 1:1000), γ-tubulin (T5326, Sigma-Aldrich, IF 1:250), p150 (610473, BD Biosciences, WB 1:10,000), NOGO-B (previously described by Rousseau et al. [66 (link)] WB 1:1000), SRF phospho-Ser103 (4261, Cell Signalling, WB 1:1000), and α-tubulin (T9026, Sigma-Aldrich, IF 1:500).
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