Log In Sign up
The largest database of trusted experimental protocols

Expression vectors

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

Expression vectors are plasmid-based DNA constructs used to express genes of interest in host cells, such as bacteria, yeast, or mammalian cells. They typically contain essential elements for gene transcription and translation, including a promoter, a multiple cloning site for inserting the gene of interest, and a selection marker for identifying successfully transformed cells.

Automatically generated - may contain errors

Lab products found in correlation

T4 dna ligase, by Thermo Fisher Scientific (1 mentions) Qiaprep spin miniprep kit, by Qiagen (1 mentions) Quikchange 2, by Agilent Technologies (1 mentions) Ecor 1 and xho 1, by Takara Bio (1 mentions)

Close spelling variants of this product

Gateway expression vectors ProQuest expression vectors PRSET-A bacterial expression vectors PSPORT6-CMV expression vectors PET303 expression vectors Expression vectors encoding the PPICZαB expression vector Bac-to-Bac Baculovirus Expression Vector System PInducer20 inducible lentiviral expression vector Promoter CMV (Cytomegalovirus) expression vector

2 protocols using expression vectors

1

Recombinant Protein Expression and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Except where otherwise indicated, all chemicals were obtained from Sigma-Aldrich. Primers, T4 DNA ligase, restriction enzymes, and expression vectors were purchased from Invitrogen. QuikChange II for site-directed mutagenesis was purchased from Agilent. The QIAprep Spin Miniprep Kit was from Qiagen. Host cells, DH5α and SoluBL21, were purchased from NEB and Genlantis, respectively. The BioMol Green™ phosphate assay kit was purchased from Enzo Life Sciences. The α-PMM1 and α-PMM2 were subcloned from commercially available cDNA.
Leonetti J.P., Mechti N., Degols G., Gagnor C, & Lebleu B. (1991). Intracellular distribution of microinjected antisense oligonucleotides.. Proceedings of the National Academy of Sciences of the United States of America, 88(7), 2702-2706.
+ Open protocol
+ Expand
2

Cloning of Expression Vectors

Check if the same lab product or an alternative is used in the 5 most similar protocols
The PCR products of the target sequences and the expression vectors (Invitrogen, Waltham, MA, USA) were ligated with Solution I ligation Enzyme (Takara, Tokyo, Japan) after digestived with the same type of restriction enzymes (EcoR I and Xho I for PIZ-BmCNN-OE plasmid; BamHI and EcoR I for PIZ-BmCDK5-OE plasmid; Takara, Tokyo, Japan). The expression efficiencies or knock out efficiencies of the cloning vectors are shown in Figure S1. All primers used in this study are listed in Table S1.
Wei Y., Zhou X., Chen P., Jiang X., Jiang Z., Dong Z., Pan M, & Lu C. (2022). BmCDK5 Affects Cell Proliferation and Cytoskeleton Morphology by Interacting with BmCNN in Bombyx mori. Insects, 13(7), 609.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!