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Ply3211 system

Manufactured by Buxco Electronics
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Sourced in United States

The PLY3211 system is a laboratory equipment designed for precise measurements and analysis. It features advanced data acquisition capabilities and comprehensive software support. The core function of this system is to provide accurate and reliable data collection and processing for scientific and industrial applications.

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5 protocols using ply3211 system

1

Measuring Lung Function in Mice

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Lung function was measured by a small animal spirometer (PLY3211 system; Buxco Electronics, Sharon, CT, USA), following the protocol described previously by our group.13 (link) In brief, each mouse was weighed first and anesthetized by intraperitoneal injection of 10% chloral hydrate (3 mL/kg) before being tracheostomized. The trachea was cannulated, and the cannula was connected to a computer-controlled small animal spirometer. Airway resistance (Raw), lung dynamic compliance (Cdyn), peak expiratory flow (PEF) and inspiratory time/expiratory time (Ti/Te) were measured and noted.
He S., Xie L., Lu J, & Sun S. (2017). Characteristics and potential role of M2 macrophages in COPD. International Journal of Chronic Obstructive Pulmonary Disease, 12, 3029-3039.
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2

Small Animal Lung Function Measurement

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Lung function including air resistance (Raw), lung dynamic compliance (Cdyn), and peak expiratory flow (PEF) was detected using small animal spirometer (PLY3211 system, Buxco Electronics, USA) as previously described [44 (link)].
Zhang Z., Zhou J., Song D., Sun Y., Liao C, & Jiang X. (2017). Gastrodin protects against LPS-induced acute lung injury by activating Nrf2 signaling pathway. Oncotarget, 8(19), 32147-32156.
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3

Comprehensive Lung Function Evaluation in Mice

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Lung function was measured using small animal spirometer (PLY3211 system, Buxco Electronics, USA) as previously described with a minor modification [10 (link)]. Briefly, the mouse was weighed, anesthetized by intraperitoneal injection of 10% chloral hydrate (3 ml/kg BW) and tracheostomized. The trachea was cannulated, and the cannula was connected to a computer-controlled small animal spirometer. Airway resistance (Raw), lung dynamic compliance (Cdyn), peak expiratory flow (PEF) and inspiratory time/expiratory time (Ti/Te) were measured.
He Z.H., Chen P., Chen Y., He S.D., Ye J.R., Zhang H.L, & Cao J. (2015). Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema. Tobacco Induced Diseases, 13(1), 6.
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4

Small-Animal Spirometry for Lung Function

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Lung-function measurement was performed using small-animal spirometry (PLY3211 system; Buxco Electronics, Wilmington, NC, USA) as previously described with a minor modification.20 (link) Briefly, the mouse was anesthetized by intraperitoneal injection of 10% chloral hydrate (3 mL/kg body weight) and tracheostomized. The trachea was cannulated and the cannula connected to the computer-controlled small-animal spirometer. Airway resistance (Raw), lung dynamic compliance (Cdyn), peak expiratory flow (PEF), and inspiratory time/expiratory time (Ti/Te) were measured according to the manufacturer’s instructions.
He Z.H., Chen Y., Chen P., He S.D., Zeng H.H., Ye J.R., Liu D, & Cao J. (2017). 5-Aza-2′-deoxycytidine protects against emphysema in mice via suppressing p16Ink4a expression in lung tissue. International Journal of Chronic Obstructive Pulmonary Disease, 12, 3149-3158.
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5

Measuring Pulmonary Function in Mice

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Pulmonary function was measured using a small animal spirometer (PLY3211 system; Buxco Electronics, Sharon, CT, USA), simply by weighing each mouse, followed by anesthesia with 1% sodium pentobarbital (0.2 mL/20 g BW) for intraperitoneal anesthesia, followed by tracheotomy, tracheal intubation, and intubation connected to a computer-controlled small animal spirometer. Airway resistance (Raw), pulmonary kinetic compliance (Cdyn), peak expiratory flow (PEF), and inspiratory time/expiratory time (Ti/Te) were measured.
Zhang L., Cheng T., Liu C., He S, & Lu J. (2024). The role and mechanism of macrophage autophagy in the experimental model of chronic obstructive pulmonary disease. Tobacco Induced Diseases, 22, 10.18332/tid/186403.
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