Nih3t3 e1 fibroblasts

Manufactured by American Type Culture Collection

Sourced in United States

NIH3T3-E1 fibroblasts are a cell line derived from mouse embryonic fibroblasts. They are a commonly used model for studying cellular processes and functions.

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Lab products found in correlation

H2030, by American Type Culture Collection (2 mentions) Hpi 1, by Abcam (2 mentions) Sb431542, by Cayman Chemical (2 mentions) Gant61, by Cayman Chemical (2 mentions) Hepg2, by American Type Culture Collection (2 mentions) Capan 1, by American Type Culture Collection (2 mentions) Carboplatin, by Cayman Chemical (1 mentions) Tgf β1, by R&D Systems (1 mentions) L3.6pl, by American Type Culture Collection (1 mentions) Cyclopamine, by R&D Systems (1 mentions) Panc 1, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions)

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NIH3T3 (543 citations)

3 protocols using nih3t3 e1 fibroblasts

Cell Culture Protocols for Cancer Research

Cited 1 time

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NIH3T3-E1 fibroblasts were obtained from ATCC (Manassas, VA, USA) and cultured as previously described [25 (link),26 (link)]. The human lung cancer cell lines A549 and H2030 were obtained from ATCC and cultured in RPMI-1640 medium containing 10% FBS and antibiotics as previously described [23 (link)]. CAPAN-1 human pancreatic adenocarcinoma cell line was purchased from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS and antibiotics as previously described [23 (link)]. Sufu-/- mouse embryonic fibroblasts were a gift from Dr. Matthew Scott of Stanford University. TGFβRI-deficient mink lung epithelial cells, R1BL17, were maintained in MEM containing 10% FBS and non-essential amino acids as described [27 (link)]. Gant61, and SB431542 were obtained from Cayman Chemical Company (Ann Arbor, MI, USA), HPI-1 was obtained from Abcam (Cambridge, UK), TGFβ1 was obtained from R&D Systems (Minneapolis, MN, USA). Carboplatin was purchased from Cayman Chemical Company (Ann Arbor, MI, USA).

Stappenbeck F., Wang F., Tang L.Y., Zhang Y.E, & Parhami F. (2019). Inhibition of Non-Small Cell Lung Cancer Cells by Oxy210, an Oxysterol-Derivative that Antagonizes TGFβ and Hedgehog Signaling. Cells, 8(10), 1297.

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Cell Culture Protocols for Various Cell Lines

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C3H10T1/2 mouse embryonic fibroblasts and NIH3T3-E1 fibroblasts were obtained from ATCC (Manassas, VA) and cultured as previously described [Dwyer et al., 2007 (link); Richardson et al., 2007 (link)]. CAPAN-1, L3.6pl, and E3LZ10.7 human pancreatic cancer cells were obtained from ATCC and cultured in DMEM containing 10% heat-inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT). The Human hepatoma cell line HepG2 was obtained from ATCC and cultured in DMEM containing 10% FBS. Mouse SUFU null (−/−) MEFs were provided by Dr. Philip Beachy at Stanford University and cultured in DMEM containing 10 FBS as previously described. LXRαβ null (−/−) MEFs were provided by Dr. Peter Tontonoz at UCLA and cultured in DMEM containing 10% FBS. GH3 cells were obtained from ATCC and cultured in Hamʹs F10 containing 2 mM Glutamine, 15% Horse Serum and 2.5 FBS. TO901317 LXR agonist was purchased from Cayman Chemicals (Ann Arbor, MI). Recombinant human Shh N-terminal peptide and cyclopamine were obtained from R&D Systems, Inc. (Minneapolis, MN), and Shh-neutralizing antibody 5E1 was obtained from Developmental Studies Hybridoma Bank (Iowa City, IA).

Wang F., Stappenbeck F., Matsui W, & Parhami F. (2016). Inhibition of Pancreatic Cancer Cell-Induced Paracrine Hedgehog Signaling by Liver X Receptor Agonists and Oxy16, a Naturally Occurring Oxysterol. Journal of cellular biochemistry, 118(3), 499-509.

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Cell Culture and Treatment Protocols

Cited 1 time

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NIH3T3-E1 fibroblasts were obtained from ATCC (Manassas, VA) and cultured, as previously described [35 (link),36 (link)]. CAPAN-1 and PANC-1 human pancreatic cancer cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% heat-inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT, USA) and antibiotics, as previously described [37 (link)]. The human lung cancer cell lines A549 and H2030 were obtained from ATCC and cultured in RPMI-1640 containing 10% FBS. The human hepatoma cell line HepG2 was obtained from ATCC and cultured in DMEM containing 10% FBS. Mouse embryonic fibroblasts (MEFs) from suppressor of fused (Sufu) null mice (Sufu^−/−) were provided by Dr. Philip Beachy of Stanford University and cultured in DMEM containing 10% FBS. Gant61, SB431542 and vismodegib (GDC0449) were obtained from Cayman Chemical, HPI-1 was obtained from Abcam. For experiments, cells were treated in a medium containing 5% FBS.

Wang F., Stappenbeck F, & Parhami F. (2019). Inhibition of Hedgehog Signaling in Fibroblasts, Pancreatic, and Lung Tumor Cells by Oxy186, an Oxysterol Analogue with Drug-Like Properties. Cells, 8(5), 509.

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