Mouse monoclonal antibody

A western blot analysis was performed as described previously [18 (link)]. Cell lysates were resolved by SDS-PAGE and transferred to a low-fluorescence PVDF membrane (Azure Biosystems, Dublin, CA, USA). Individual proteins were labelled with specific antibodies and visualized by chemiluminescence and SuperSignal^® West Femto Maximum Sensitivity Substrate (Thermo Scientific, Waltham, MA, USA) or by near infrared fluorescence using Azure Biosystems c600. IRF-3 was detected with a mouse monoclonal antibody (BioLegend, San Diego, CA, USA, dilution 1:1,000) and a peroxidase-conjugated goat anti-mouse antibody (Sigma Co., St. Louis, MO, USA, dilution 1:10,000); phosphorylated IRF-3 was detected with a rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, USA) and a IR 700-conjugated goat anti-rabbit antibody (Azure Biosystems, Dublin, CA, USA, dilution 1:10,000); β-actin was detected with a rabbit polyclonal antibody (Abcam, Cambridge, UK, dilution 1:2,500) and peroxidase-conjugated goat anti-rabbit antibody (MP Biomedicals-Cappel, Solon, OH, USA, dilution 1:10,000); PARP-1 was detected with a rabbit polyclonal antibody (Santa Cruz Biotechnology, Dallas, TX, USA, dilution 1:500) and peroxidase-conjugated goat anti-rabbit antibody (MP Biomedicals-Cappel, Solon, OH, USA, dilution 1:10,000).