Hanks balanced salt solution hbbs

The Remdesivir for formulation was purchased from Medkoo Biosciences (Research Triangle Park, NC, USA). Remdesivir, GS-441524, and its heavy isotope internal standards were purchased from Alsachim (Illkirch-Graffenstaden, France). Lactose monohydrate, leucine, polysorbate 20, acetonitrile (HPLC grade), and trifluoracetic acid were purchased from Fisher Scientific (Pittsburgh, PA, USA). D-Mannitol was bought from Acros Organic (Fair lawn, NJ, USA). Dipalmitoylphosphotidylcholine (DPPC) was purchased from Avanti Polar Lipid, Inc. (Alabaster, AL, USA). Cholesterol, albumin, transferrin, ascorbic acid and Hanks’ Balanced Salt solution (HBBS) were purchased from Sigma-Aldrich (St.Louis, MO, USA). Sulfobutylether-beta-cyclodextrin (SBECD, Captisol^®) was kindly provided by CyDex Pharmaceuticals, Inc. (Lawrence, KS, USA) High-resistance Monodose RS00 dry powder inhalers were kindly provided by Plastiape S.p.A. (Osnago, Italy).

OLEA (≥90% purity) was purchased from Toronto Research Chemicals (North York, ON, Canada). Naproxen and phenol red were purchased from Sigma-Aldrich (Madrid, Spain). Heparin sodium salt from porcine intestinal mucosa, Hanks’ balanced salt solution (HBBS), and HEPES 1M solution were also obtained from Sigma-Aldrich (Madrid, Spain). Pentobarbital sodium 200 mg/mL (Dolethal) was supplied by Vetoquinol (Madrid, Spain) and isoflurane by Laboratorios Esteve (Barcelona, Spain). The solvents acetonitrile and methanol, and the chemical formic acid were acquired from PanReac AppliChem (Panreac Quimica SLU, Barcelona, Spain). Finally, a Milli-Q purification system was used to obtain ultrapure water (Millipore, Bedford, MA, USA).